Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"isotope labelling"”.
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Orientation and chemistry of alkoxides on copper(111)
… as aldehydes. The influence of deuterium isotope labelling, selective fluorination, and chain length on this mechanism has been studied. Temperature Programmed Reaction (TPR) spectra for the reactions of ethoxides and 1-propoxides and their selectively deuterated counterparts reveal both …
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Liverwort-Fungal Associations: Novel Symbioses In Polar Regions?
… their liverwort host, a dual 1 5N and 1 4C isotope labelling experiment was established, with NanoSIMS being utilized to visualise the flow of N into liverwort tissue. I found that fungal colonisation was frequent in the liverworts surveyed, with species from the Serendipitaceae being among …
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Experimental and Theoretical Study of Carbon Dynamics in an Algal-Bacterial Co-Culture
… were studied experimentally using stable isotope labelling and Secondary Ion Mass Spectrometry (SIMS), providing temporal measurements at the single cell level of the carbon assimilation by the algae and its transfer to bacteria. In the mathematical ecology of interacting species, …
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Nitrogen uptake patterns by snow addition in a sub-Arctic dry heath tundra ecosystem
… Through snow fence experiments and 15N isotope labelling and simulations of carbon fluxes with the CoupModel, I found that a longer growing season positively impacted plant productivity, carbon accumulation, and nutrient assimilation. Both evergreen and deciduous plants benefited from an …
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A quantitative characterisation of phospholipid composition and biosynthesis in HeLa cells and nuclei - a mass spectrometry study
… Using a combination of ESI-MS and stable isotope labelling, the biosynthesis of PC was monitored in both whole cells and nuclei. The molecular species composition of newly synthesised PC closely resembled that of endogenous PC. Biosynthesis of PC was found to continue in nuclei that had …
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Multi-omic Approaches to Investigate the Inflammatory Response in Activated Macrophage Cells
… metabolism.<br/>Furthermore, a novel stable isotope labelling methodology was developed using<br/>deuterium oxide (2H2O) in conjunction with high resolution mass spectrometry for the simultaneous measurement of the rates of synthesis of individual lipids and proteins on a cellular-wide scale. …
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Investigating the molecular mechanisms of the interactions between Lactobacillus reuteri strains and intestinal mucus
… a quantitative proteomic approach, stable isotope labelling with amino acids in cell culture (SILAC), was used to identify novel mucus binding protein candidates. This was achieved through detection of increased cell surface protein expression when L. reuteri was grown in presence of …
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A screen for phosphatidylinositol 3,4-bisphosphate and other 3-phosphoinositide effector proteins
… In addition, this screen utilises stable isotope labelling with amino acids in cell culture (SILAC) to differentially label cells stimulated in the absence and presence of the PI 3-kinase inhibitor wortmannin. The integration of these techniques provides a ratio-metric readout, allowing …
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The role of latent membrane protein 2 in the pathogenesis of Epstein-Barr virus associated malignancy
… these cells was confirmed. A new approach stable isotope labelling amino acid in cell culture (SILAC) to identify interacting proteins which combined with LC-MS/MS analysis was performed and 10 novel LMP2A interactors and 20 novel LMP2B interactors were identified. All these potential interactions …
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Development of a Software Package for the Quantitative Analysis of Proteomic Mass Spectrometry Datasets Labelled with Nitrogen-15
Elemental metabolic labelling using 15N stable isotopes is a technique used in peptide-centric proteomics that allows samples to be mixed before preparation and analysis (minimising technical variance) without introducing sample ambiguity to the results. Labelling with 15N induces a mass shift in …
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Extending the Boundaries of the Usage of NMR Chemical Shifts in Deciphering Biomolecular Structure and Dynamics
… the advent of recent techniques for specific isotope labelling of protein side-chain atoms, which make large biomolecules accessible to NMR techniques. Through their incorporation as restraints in molecular dynamics simulations, the chemical shifts predicted by the approach described in this …
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Monitoring and regulating cyanobacterial metabolites (microcystins and geosmin) in aquatic systems.
… effect was observed applying a novel stable isotope labelling (15N) method. Microcystin- LR has been shown to stimulate culture growth, limit microcystin synthesis, and affect the distribution of microcystin-LR between the intra- and extracellular matrices. Furthermore the control of …
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Borenium Cations as Catalysts for the Reduction of Organic Molecules and Mechanistic Investigations into their Mode of Operation
… mechanisms. Stoichiometric experiments and isotope labelling using deuterated pinacol borane demonstrated that the nucleophilic counterion was not a kinetically relevant reducing agent under the reaction conditions. Furthermore, these reactions and the use of an isolable iminium ion as a …
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The role of JAK1 and JAK3 in CD8<sup>+</sup> effector T cells
… mass spectrometry analysis of SILAC (stable isotope labelling with amino acids in cell culture) labelled CTL. Tofacitinib regulated a selective number of phosphorylation sites, with less than 1.2% of the CTL phosphoproteome significantly regulated by tofacitinib treatment following 4hrs …
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The cue induced axonal nascent proteome and its translational control mechanisms in neural wiring
… of pure material. By combining pulsed Stable Isotope Labelling by Amino acids in Cell culture (pSILAC) with Single-Pot Solid-Phase-enhanced Sample Preparation (SP3), I have established an approach to characterize the nascent proteome from quantity-limited somaless retinal axons (~2μg) on an …
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Catalytic Light Hydrocarbon Upgrading under Methane Environment
… using Ag-Ga/HZSM-5 catalyst and verified using isotope labelling and GC-MS analysis as well as monitoring the reaction heat effect. The co-aromatization reaction between olefins and methane is further investigated by isotopic labelling method and evidenced by the escalated exothermic feature. …
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Development and Application of Mass Spectrometry Based Methodologies to Study Ubiquitin-Like Modifiers
… identifications has been achieved through UBL isotope labelling. In vitro substrate modification with isotopically light and heavy UBL generates a characteristic isotopic doublet enabling isopeptides to be distinguished at the MS1 level. Candidate peptide identity can be assigned using …
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Metabolomic Insights into the Pharmacological and Genetic Inhibition of Cyclooxygenase-2
… and untargeted metabolomic techniques, stable isotope labelling and quantitative reverse transcription polymerase chain reaction (RT-qPCR) to i) profile the metabolic changes induced by celecoxib and rofecoxib, in the mouse; ii) specifically interrogate the effect of celecoxib, rofecoxib and …
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Quantitative proteomic analysis of cellular senescence: from systems biology to targeted signaling pathways
… proteomic study applying SILAC (stable isotope labelling of amino acids in cell culture) indicated that a large number of proteins were up-regulated (179 of 2206 proteins identified) in senescent cells. These proteins were categorized into several functional groups, functioning in RNA …
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Studies of viral and cellular proteins involved in herpes simplex virus type-1 egress
… by mass-spectrometric analysis utilising stable isotope labelling with amino acids in cell culture (SILAC) medium. Among the cellular interactome obtained, Nipsnap1 was validated by co-precipitation assays from both infected and transfected cells, and furthermore using cell free systems, …
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