Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 50 for “"isothermal titration calorimetry (ITC)"”.
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Expression and purification of HGXPRT from Plasmodium falciparum and characterisation of its interaction with an effector chalcone
… namely gel filtration HPLC, photolabelling, and Isothermal Titration Calorimetry (ITC) for ultimately characterising the interaction of the effector chalcones with the protein.
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Towards an understanding of peptide-inorganic interactions
… with dissipation monitoring (QCM-D) and isothermal titration calorimetry (ITC).
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Herbicide Mediated Selection of Sulfonamide Resistant E. coli
… can select for antibiotic resistant bacteria, isothermal titration calorimetry (ITC) was used to assess the binding affinity of asulam with the target protein of sulfas, dihydropteroate synthase (DHPS). ITC confirmed asulam interacts with DHPS, and in vitro directed evolution experiments showed …
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Developing Materials for Rare-Earth–Element Chelation: Synthesis, Solution Thermodynamics, and Applications
… elucidated the thermodynamics of binding using isothermal titration calorimetry (ITC) to gain insight into the specific relationship between polymer structure and metal binding. ITC enables the direct measurement of the binding affinity (Ka), enthalpy changes (ΔH), and stoichiometry of the …
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Mutational Analysis of HIV-1 Nucleocapsid Protein and Methods Development for Aptamer Discovery
… RNA, were measured using tryptophan fluorescence titration assay and/or isothermal titration calorimetry (ITC) assay. Most mutants show tolerance of the mutation but some mutants, including F16A, I24A and K14E-E21K, suffered moderate to significant loss of affinity for SL3 RNA, indicating the …
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Synthesis of [beta]-heteroaryl propionates via trapping of carbocations with [pi]-nucleophiles, efforts towards the total synthesis of acutumine, and the design, synthesis, and thermodynamics of protein-ligand interactions at the Src SH2 domain
… appropriate flexible controls via the use of isothermal titration calorimetry (ITC) to determine the thermodynamics differences of binding between the two.
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Interactions of functionalized vesicles in the presence of Europium (III) Chloride
… interactions. Measurements performed with isothermal titration calorimetry (ITC) revealed that interaction between EuCl3 and ß-diketone receptors was characterized by a molar enthalpy 126 ± 5 kcal/mole and an equilibrium binding constant 26 ± 4 mM-1. The results indicate a molecular complex …
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Isothermal Titration Calorimetry Uncovers Substrate Promiscuity of Bicupin Oxalate Oxidase
… assays often confound data interpretation. Isothermal titration calorimetry (ITC) measures heat changes that occur during a reaction. Therefore, ITC can measure reaction rates where both the substrates and products are spectrophotometrically silent and when the reaction products are unknown. …
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Structural Studies on Calcium/calmodulin-dependent Activation of Eukaryotic Elongation Factor 2 Kinase
… magnetic resonance (NMR) spectroscopy and isothermal titration calorimetry (ITC), coupled with mutational strategies to characterize structural and thermodynamic nature of the binding of Ca<sup>2+</sup>-CaM onto eEF-2K. Our data clarify the roles of Ca<sup>2+</sup> and each lobe of CaM that …
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Investigating antimicrobial resistance mechanisms in Neisseria gonorrhoeae using peptide probes
… pump are also ligands for the regulator MtrR. Isothermal titration calorimetry (ITC) was used to show that MtrR binds commercial antibiotics and antimicrobial peptides. -lactam antibiotics not only bind MtrR but are hydrolysed by the multidrug protein. Evidence for this novel enzymatic …
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Calorimetric studies of small-molecule adsorption to carbon nanotubes
Isothermal titration calorimetry (ITC) was developed as a technique for qualitatively comparing the heat of absorption of small molecules to single-walled carbon nanotubes (SWCNTs). In agreement with other studies, it was shown that polyaromatic hydrocarbons (PAHs) that can achieve a greater degree …
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Structural Characterization of the interaction between divalent cations and the novel Schistosoma mansoni Universal Stress Protein G4LZI3
… protein with 3 metallic divalent cations using Isothermal Titration Calorimetry (ITC). Additionally, bioinformatics was employed to predict the secondary structure of the protein, generate a 3-dimensional model of the protein and use it for docking the divalent cations. Moreover, the interacting …
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A thermodynamic study of the effects of membrane composition on the binding affinity of annexin a5.
… phosphatidylserine, and calcium ion. We find by Isothermal Titration Calorimetry (ITC) that annexin a5 binds calcium ions with much higher affinity in the presence of non-cholesterol containing neutral and acidic phospholipid mixtures ((60:40) (POPC:POPS)) relative to solution state (without …
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rRNA transcription as potential therapeutic target in triple negative breast cancers
… PMR-116 by using biochemical methods including Isothermal Titration Calorimetry (ITC).<br/><br/><br/>
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Elucidation of Battery Electrolyte Coordination Sphere Thermodynamics via Calorimetric and Potentiometric Titrations
… a finer picture of the Li+ coordination sphere, isothermal titration calorimetry (ITC) and potentiometric titrations (PT) were employed with a set of exemplar electrolytes to probe the thermodynamic evolution of the Li+ coordination complex as weak solvent is displaced by a stronger solvent in …
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Directing Polymer-Metal Binding Interactions by Modifying Polymer and Solvation Structure
… changes affected metal-chelation we turned to isothermal titration calorimetry (ITC) to measure the solution thermodynamics of these interactions directly. We studied the effect of the broader solution environment by changing the solution composition and solvent to find that both impacted the …
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The Cocaine-Binding Aptamer: A Thermodynamic and Structural Overview of Specific and Non-Specific Binding Interactions
Isothermal Titration Calorimetry (ITC) studies were conducted wherein variances in the heat capacity of binding (Cp) among variants of the cocaine-binding aptamer were suggestive of two distinct binding mechanisms. Aptamer variants containing 6 base pairs in stem 1 are pre-folded and show little …
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Thermodynamic and Spectroscopic Investigations of Novel Antimicrobial Peptides containing Unnatural Amino Acids with Model Membrane Systems
… onto these peptides. Circular Dichroism (CD), isothermal titration calorimetry (ITC) and calcein leakage assays were conducted and it was determined that there are two distinctly different mechanisms of binding occurring between this series of peptides and zwitterionic and anionic membrane …
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Improving Photocatalytic Hydrogen Production of Ru,Rh,Ru Supramolecular Complexes in Aerobic Aqueous Solutions
… and polyelectrolytes was probed using isothermal titration calorimetry (ITC). ITC studies reveal the driving forces of aggregate formation, providing new insight into the intermolecular forces that lead to increased photocatalytic efficiency and stability in the presence of water …
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De Novo Design and Stabilization of β-Sheet Peptides for Biomimicry of ORR Enzymes
… of circular dichroism (CD), UV-Vis spectroscopy, isothermal titration calorimetry (ITC), electron paramagnetic resonance (EPR), diffusion-ordered spectroscopy (DOSY), and single-crystal X-ray diffraction (SC-XRD). The peptides are analyzed both in their apo and metalated forms. This work features …
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