Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 109 for “"isothermal titration calorimetry"”.
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Isothermal Titration Calorimetry Uncovers Substrate Promiscuity of Bicupin Oxalate Oxidase
… assays often confound data interpretation. Isothermal titration calorimetry (ITC) measures heat changes that occur during a reaction. Therefore, ITC can measure reaction rates where both the substrates and products are spectrophotometrically silent and when the reaction products are unknown. …
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An isoperibol calorimeter for the investigation of biochemical kinetics and isothermal titration calorimetry
Isothermal titration calorimetry is a technique used to measure the enthalpy change associated with a molecular binding interaction. From these data, the binding constant for the reaction can be determined. In the scope of a larger project to design a high sensitivity instrument for collecting such …
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Isothermal Titration Calorimetry Studies Of Protein-Mediated Interactions And Preliminary Structural Studies Of Tandem Pdz1-2 Domain Of Psd-95 Protein
… analysis of PDZ domain binding using calorimetry; (2) X-ray crystallographic structural studies of a PDZ dual domain; and (3) thermodynamic analysis of frataxin binding to iron. The specific goal of the research conducted with the PDZ domains was to understand the mechanism of action …
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Expression and purification of HGXPRT from Plasmodium falciparum and characterisation of its interaction with an effector chalcone
… namely gel filtration HPLC, photolabelling, and Isothermal Titration Calorimetry (ITC) for ultimately characterising the interaction of the effector chalcones with the protein.
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Protein Dynamics and its Correlation to Protein Activity and Stability
… scattering, circular dichroism, UV absorption, isothermal titration calorimetry and X-ray crystallography was undertaken to examine the current paradigm using the enzyme: dihydrofolate reductase as a model. As inferred by neutron spectroscopy, the binding of MTX influences the dynamical behavior …
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Structure and Function of Colicin E5 Ribonuclease Domain
… in a 50% increase of fluorescence emissions. The isothermal titration calorimetry studies showed a -10 kcal/mol enthalpy change upon the formation of ColE5•ImmE5 complex. The mutational studies of ImmE5 indicated that ImmE5 K4 and D95 contributes a larger inhibitory effect toward ColE5. The …
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Characterization of the Chemoreceptors in the Bacillus Subtilis Chemotaxis System
… identification of a zinc ion binding area, using isothermal titration calorimetry. In addition, in vivo chemotactic assays showed that altering the charges of the methylation sites on the chemoreceptors can have a significant effect on the activity of the receptor. Furthermore, individual …
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Thermodynamic Investigation into the Binding Properties of Cardiac Troponin (Human and Bovine)
… C subunit of the cardiac muscle protein using isothermal titration calorimetry. We have been able to successfully observe Ca[superscript]2[superscript]+ binding to the apo form of Bovine Cardiac TnC (BVCTnC) as well as the apo form of Human Cardiac TnC (HCTnC). Familial Hypertrophic …
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Saccharide recognition : boronic acids as receptors in polymeric networks
… of saccharide binding by means of isothermal titration calorimetry and displacement assay (ARS) to enhance the association constant to saccharides at pH 7.4 • Enhancement of selectivity in polymeric systems by means of molecular imprinting using fructose as template and a …
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The Utility of 7-Deazaguanine: Part I. High Affinity, High Fidelity Modules for Supramolecular Chemistry, And, Part II. Molecular Recognition of Uracil and Thymine
… binds T by intercalation and hydrogen-bonding. Isothermal titration calorimetry experiments gave a K d of 5.4 +/- 0.6 muM to the T bulge.
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Investigation of mosA, a protein implicated in rhizopine biosynthesis
… compounds interacting with MosA as observed by isothermal titration calorimetry. Initial investigation into MosA’s methyltransferase activity began with 2-OB’s interaction with the enzyme. Inhibition experiments determined 2-OB is a competitive inhibitor with respect to pyruvate of the DHDPS …
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Design, synthesis, and thermodynamic evaluation of peptidomimetic ligands binding to the Src SH2 domain
… amino acid analog as well as a flexible analog. Isothermal titration calorimetry studies were performed to assess the effects of ligand structure on protein-ligand binding energetics.
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Towards an understanding of peptide-inorganic interactions
… with dissipation monitoring (QCM-D) and isothermal titration calorimetry (ITC).
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Structural Studies of Enzymes Involved in Drug Resistance and Antibiotic Biosynthesis
… bioassay and enzyme-substrate affinity based on isothermal titration calorimetry. Our structural and biochemical data helps to characterize EreB and elucidate its catalytic mechanism, providing a valuable framework to develop efficient inhibitors of EreB for pharmaceutical application.
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Herbicide Mediated Selection of Sulfonamide Resistant E. coli
… can select for antibiotic resistant bacteria, isothermal titration calorimetry (ITC) was used to assess the binding affinity of asulam with the target protein of sulfas, dihydropteroate synthase (DHPS). ITC confirmed asulam interacts with DHPS, and in vitro directed evolution experiments showed …
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Developing Materials for Rare-Earth–Element Chelation: Synthesis, Solution Thermodynamics, and Applications
… elucidated the thermodynamics of binding using isothermal titration calorimetry (ITC) to gain insight into the specific relationship between polymer structure and metal binding. ITC enables the direct measurement of the binding affinity (Ka), enthalpy changes (ΔH), and stoichiometry of the …
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Mutational Analysis of HIV-1 Nucleocapsid Protein and Methods Development for Aptamer Discovery
… RNA, were measured using tryptophan fluorescence titration assay and/or isothermal titration calorimetry (ITC) assay. Most mutants show tolerance of the mutation but some mutants, including F16A, I24A and K14E-E21K, suffered moderate to significant loss of affinity for SL3 RNA, indicating the …
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PHYSICAL CHEMISTRY OF COLLOIDAL-NANOPARTICLE INTERACTIONS
… contributions through circular dichroism and isothermal titration calorimetry. The dominant entropic pathway of complexation, showed that the assembly of supra-colloid microstructures by using nano-particles and biopolymers as building blocks is not limited by unfavorable enthalpic …
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Dipeptides as potential anti-flammatory drugs for rheumatoid arthritis
… studied using glass electrode potentiometry and isothermal titration calorimetry at 25 °C and an ionic strength 0.15 M (NaCl). The terminal amine of the sarcosine peptides is more basic than the glycine analogues. The methyl group on the terminal amine (for sarcosine peptides) does not affect the …
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Discovering LC3-interacting region (LIR) motifs in hemorrhagic fever viruses: Implications for host autophagy and viral replication.
… region, which are hot spots for LIR motifs. Isothermal titration calorimetry, X-ray crystallography, co-immunoprecipitation, and co-localization experiments confirmed that the C-terminal LIR motif (NSs4) interacts with all six human LC3 proteins. We identified phenylalanine 261 (F261) in NSs4 …
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