Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 743 for “"isoform"”.
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Quantitative isoforrm profiling & isoform convergence
… of quantitative exon profiling and quantitative isoform profiling which take advantage of a single-molecule based technology [Mit99]. Furthermore, we extend this paradigm to include a novel unified platform-called Isoform Convergence-to qualify particular isoforms as candidate diagnostic markers, …
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Alternative isoform regulation in myotonic dystrophy
… widespread deleterious changes in transcriptome isoform usage. We developed a method for studying transcriptomes, RNAseq, which provides a high resolution, digital inventory of gene and isoform expression. By applying RNAseq to human tissues and cell lines, we discovered that essentially 92-94% …
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Characterization of KPAP, a Novel Pokeweed Antiviral Protein Isoform
… levels did not behave the same way as other isoforms under phytohormone stimulated biotic stress, however, they showed a slight decrease in drought. In this study, I provide preliminary groundwork on the KPAP gene model, expression in abiotic stresses, protein structure, depurination activity …
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Investigation of ARNT isoform-specific regulation of AHR signaling
… is alternatively spliced into two distinct isoforms, isoform 1 and 3. Although ARNT is found to be critical in immunity, xenobiotic, and hypoxic response, ARNT isoform-specific function has yet to be investigated. We previously demonstrated that primary lymphocytes express both of these …
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Gating Mechanisms of The Canonical Trp Channel Isoform Trpc4
<p>Non-selective cation channels formed by Transient Receptor Potential Canonical (TRPC) proteins play important roles in regulatory and pathophysiological processes. These channels are known to be activated downstream from phospholipase C (PLC) signaling. However, the mechanism by which the PLC …
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PP2A-B56 isoform specificity at the centromere and kinetochore
… are split between distinct subsets of B56 isoforms. PP2A-B56γ and PP2A-B56δ localize to the outer kinetochore (via BUBR1), whereas PP2A-B56α and PP2A-B56ε localize to the centromere (via Sgo2). The differential localization observed is due to a difference in affinity for the receptors: …
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Isoform-Specific Roles of Extracellular Signal-Regulated Kinases in Pain
ABSTRACT OF THE DISSERTATION Isoform-specific roles of Extracellular Signal-Regulated Kinases in pain by Benedict Joseph Alter Doctor of Philosophy in Biology and Biomedical Sciences Neurosciences Washington University in St. Louis, 2012 Professor Robert Gereau, Chairperson The extracellular …
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Investigating the isoform-specific role of phosphoinositide 3- kinase p110delta
… The PI3K family is comprised of multiple isoforms that are divided into three main classes; class I, II and III. All class I PI3Ks are heterodimers consisting of a ~110 kDa catalytic subunit associated with a regulatory subunit. Class I PI3Ks are further subdivided into class IA and class …
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Function and inhibition of the mitochondrial O-GlcNAc transferase isoform
… suggested the presence of a mitochondrial OGT isoform (mOGT) in human cell lines in addition to the well-characterised nucleocytoplasmic one (ncOGT). Since this report more than one decade ago, this putative mOGT has not been studied further. Similarly, hundreds of O-GlcNAcylated …
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Isoform-Selective HDAC Inhibition for the Treatment of Lupus Nephritis
… the expression and function of different HDAC isoforms in immune cells. Due to the ability of HDAC inhibition to decrease markers of SLE disease as well as alter B and T cell development and differentiation, we sought to determine if specific HDAC isoforms are altered in lupus vs non lupus mice …
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Bayesian Modeling for Isoform Identification and Phenotype-specific Transcript Assembly
… on two research topics in this dissertation: isoform identification and phenotype-specific transcript assembly. For isoform identification, we develop a computational approach, SparseIso, to jointly model the existence and abundance of isoforms in a Bayesian framework. A spike-and-slab prior …
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Structure Activity Relationship Studies on Isoform Selective Sphingosine Kinase Inhibitors
… of sphingosine (Sph) and is catalyzed by the two isoforms of sphingosine kinase (SphK1 and SphK2). Therefore, SphKs are a potential therapeutic target; however, the physiological role of SphK2 is still emerging. In order to determine the role of SphK2 in vivo, more potent and selective small …
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Isoform-Specific Expression During Embryo Development in Arabidopsis and Soybean
… on the domain composition of resulting protein isoforms. The majority of splice variants pairs encode proteins with identical domains or similar domains with truncation and in less than 10% of the cases alternative splicing results in gain or loss of a conserved domain. I constructed several …
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Software for Estimation of Human Transcriptome Isoform Expression Using RNA-Seq Data
… capable of handling multireads and quantifying isoforms on a more global level. Current software available for these purposes uses various forms of parameter alteration in order to work with multireads. Many still analyze isoforms per gene or per researcher determined clusters as well. By doing …
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Identifizierung und Charakterisierung einer tumorentitätspezifisch überexprimierten Protein-Isoform eines bisher unbekannten Gens
… zu drei verschiedenen Carboxy-terminalen Protein-Isoformen führen. Zu LUMA orthologe, bisher unbekannte Nukleotid- bzw. Aminosäuresequenzen wurden in verschiedenen Organismen identifiziert und weisen auf eine evolutionäre Konservierung und funktionelle Bedeutung des bisher unbekannten Gens hin. Da …
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Mena 11a-isoform specific regulation of actin cytoskeleton organization and cell behavior
… is alternatively spliced, producing protein isoforms with distinct functions. Here, we characterize the expression, function, and regulation of the Mena11a splice isoform at the molecular and cellular level. We find that Menal 1 a is enriched in epithelia and muscle in both embryonic and …
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Isoform-Specific Variant and Expression Analysis of Epigenetic Genes in Neurodevelopment and Disease
Isoform-Specific Genetic Analysis of Epigenetic Machinery Genes in Neurodevelopment and Disease Úlfur Benedikt Fjölnisson¹, Katrín Möller¹, Hans Tómas Björnsson¹,²,³ ¹ Department of Medicine, University of Iceland, Reykjavík, Iceland ² Johns Hopkins University, Baltimore, USA ³ University Hospital, …
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Role of Exchange Protein Directly Activated by cAMP Isoform 1 in Energy Homeostasis
… directly activated by cAMP (Epac). Epac has two isoforms, the ubiquitously expressed Epac1 and the more tissue restricted Epac2. The biological functions of Epac1 have been revealed, including cardiac stress, chronic pain, cancer and infections. However, the roles of Epac1 in energy balance are …
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Elucidating Isoform Specific Roles of Calpain-1 and Calpain-2 in Breast Cancer
… us to analyze the functions of these two calpain isoforms in the MDA-MB-231 triple negative breast cancer model cell line and to determine which isoform is responsible for the CAPNS1-knockdown related cancer cell phenotypes seen previously. We used the panel of knockout and rescue cell lines for …
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