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Showing 1 to 20 of 69 for “"isoelectric point"”.
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ΜΕΛΕΤΗ ΤΗΣ ΒΙΟΣΥΝΘΕΣΗΣ ΤΩΝ ΠΟΛΥΑΜΙΝΩΝ ΣΤΑ ΒΛΑΣΤΗΜΕΝΑ ΣΠΕΡΜΑΤΑ ΚΡΙΘΑΡΙΟΥ
… WEIGHT OF CHROMATIN-BOUND ODC IS 55,000 WITH ISOELECTRIC POINT 5.2 AND KM TO L-ORNITHINE 0.36 MM. CYTOSOLIC ODC HAS BEEN PURIFIED 4,000-FOLD AND ITS MOLECULAR WEIGHT IS ONE OF THE PROTEIN WITH MOLECULAR WEIGHT OF 55,000 OR 37,000. THE ISOELECTRIC POINT OF CYTOSOLIC ODC IS 5,5 AND KM TO L …
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Sequence-specific recognition of DNA by RSRI endonuclease of Rhodobacter sphaeroides, an isoschizomer of EcoRI
… to EcoRI, no tetrameric form was observed. The isoelectric point of the endonuclease was determined to be 7.0-different than the value of 6.3 reported for EcoRI.
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Studies on the cone pigment, iodopsin
… for the other cone pigments too) has a high isoelectric point value.
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Mathematical theory of isoelectric focusing.
… model describing transient processes in isoelectric focusing (IEF) of M biprotic ampholytes is proposed. The problem consists of nonlinear partial differential equations and algebraic equations under nonlinear boundary conditions. Different models of IEF have been studied. For each model …
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Mechanisms of Immunosuppression in Murine Malaria
… glycoprotein (M.W. 27,000 daltons), possessing isoelectric point of 7.00. The suppressor activity could be abrogated by proteolytic enzyme digestion but was not abolished by periodate oxidation. The purified suppressor substance did not reduce the PFC response to DNP-Ficoll, a T-independent …
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Arginase from Bacillus caldovelox
… a hexameric subunit structure. It has an isoelectric point of 5.4, a pH optimum for activity of 9 (at 60°C in 0.1M CAPS/NaOH buffer) and requires a divalent cation for activity. The purified enzyme contains 1.2 Mn²⁺ ions and 0.17 Fe²⁺ ions per subunit and is activated ~30% by Mn²⁺. The …
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RNAi-related genes in Toxoplasma gondii.
… molecular weight of 58.5 kDa and an estimated isoelectric point (pI) of 9.4. Using bioinformatic methods, we found significant homology between the Piwi domains of TgAgo and previously reported Argonaute proteins, but were unable to locate a PAZ domain. Polyclonal antibodies raised against …
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Cloning and characterization of neuraminidase from Salmonella typhimurium LT-2 and its relationship to neuraminidase from other bacteria
… purified. The purified NANase is 41.3 kD with an isoelectric point of approximately 9.0. The enzyme does not require Ca$\sp{++}$ for maximal activity and is not affected by EDTA or EGTA. S. typhimurium NANase is insensitive to N-acetylneuraminic acid (NeuNAc) and dithiothreitol. Compared to many …
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Anisotropic and tunable characteristics of the colloidal behavior of metal oxide surfaces
… with particular interest in changes of the isoelectric point (iep). Differences in iep as a function of orientation are explained in terms of differences in both the coordination number and density of (Lewis) acidic and basic sites on the surface. The results were supported by angle-resolved …
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Electrically evoked motions of the isolated mouse tectorial membrane
… A 2-D lateral mapping of motion at several points on the TM surface shows that the TM expands near the negative electrode and contracts near the positive electrode with a stationary pivot point between the two electrodes. Lowering the pH in the bath surrounding the TM from 7.3 to 4.07 …
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The colloid and surface chemistry of allophane
… was determined by potentiometric titration; its point of zero charge was found to be below pH 5. However, determination of the surface charge below pH 6 was complicated by the dissolution of aluminium. The isoelectric point of the same material, as determined by electrophoresis, was near pH 6. …
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Proteomics approach to investigate separation behavior of proteins in ion-exchange chromatography
<p>In the present study, protein isoelectric point (pI), molecular weight (MW) and partitioning coefficient (K) were characterized for a mixture of proteins using a three-dimensional (3D) method which combined hydrophobic partitioning with two-dimensional (2D) electrophoresis. Protein pI and MW …
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Comparative enzymology of wheat germ aspartate transcarbamoylase and related studies
… have been reviewed. The amino acid composition, isoelectric point and peptide maps of purified wheat germ aspartate transcarbamoylase have been elucidated and compared to the E.coli enzyme. Three types of essential amino acid residues have been demonstrated by chemical modification reagents. …
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Hydration and activity studies of bovine skeletal muscle proteins and wheat gliadins by nuclear magnetic resonance
… at low pH, with hydration being minimal at the isoelectric point (5.5). The molecular dynamics of hydration water and ions bound to myosin occurred on a time scale of tens of picoseconds. The analysis of $\sp{23}$Na NMR studies of Na$\sp+$ binding to myofibrillar proteins reveals the presence of …
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An evaluation of continuous ultrasonic coagulation employing a ball-clay hydrosol
… will adhere, provided they are at or near their isoelectric point. It has been demonstrated previously that ultrasonic energy will increase the intensity as well as the number of collisions of colloidal and near colloidal particles and consequently hasten their rate of coagulation. However, since …
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Interfacial behavior of RNA-free MS2 bacteriophage particles
… were studied for these two nanoparticles. The isoelectric point of MS2 and RNA-free MS2 were found to be 3.4 and 3.2 respectively, found by varying pH at a constant ionic strength. The electrophoretic softness, found by varying ionic strength and constant pH, found that MS2 was 2.1 nm and that …
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Changes in proteins during the cell cycle of Physarum polycephalum
… on the gels had a 32,000 molecular weight and isoelectric point of 4.9. This protein increased in concentration during early prophase, peaked in metaphase and decreased by a factor of five by one hour after metaphase. The identity of this protein is not known. Four forms of actin were purified …
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Dispersed and deposited polyelectrolyte complexes and their interactions to chiral compounds and proteins
… weight, charge density) and proteins (e.g. isoelectric point, size, hydrophobicity), PEC showed different uptake characteristics towards different proteins. Electrostatic and hydrophobic interaction as well as counterion release force were considered as possible driving forces for protein …
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