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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 57 for “"isoelectric focusing"”.
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Mathematical theory of isoelectric focusing.
… model describing transient processes in isoelectric focusing (IEF) of M biprotic ampholytes is proposed. The problem consists of nonlinear partial differential equations and algebraic equations under nonlinear boundary conditions. Different models of IEF have been studied. For each model …
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Micro free-flow isoelectric focusing
… and increase assay sensitivity. Rapid free flow isoelectric focusing (FF-IEF) is achieved in a microfluidic device by isolating the electrodes from the focusing region with porous buffer regions. Moving the electrodes enables the use of large electric fields without the detrimental effects of …
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Applications and Advancements of Dynamic Isoelectric Focusing
… is to explore the capabilities of DIEF. Dynamic isoelectric focusing (DIEF) is a separation technique invented by Dr. Luke Tolley. It is similar to capillary isoelectric focusing except it uses four high voltage electrodes to form a pH gradient instead of only two. The additional two electrodes …
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MICROFLUIDIC DYNAMIC ISOELECTRIC FOCUSING COUPLED TO MATRIX ASSISTED LASER DESORPTION/IONIZATION MASS SPECTROMETRY
… device has been designed to perform dynamic isoelectric focusing (DIEF) based protein separation with digital sample deposition directly on a MALDI target for offline analysis. DIEF is related to capillary isoelectric focusing which and can facilitate the interface without the loss of the …
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Genetic polymorphism of the human apolipoproteins A and E
The technique of horizontal, thin layer isoelectric focusing was used to demonstrate the polymorphism of the apolipoproteins E, A-I, A-II and A-IV. Polymorphic forms of apo E were detected after ultracentrifugation of plasma, delipidation of VLDL and isoelectric focusing of the remaining protein. …
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Green Pea Peroxidases: Their Catalytic Properties and Thermal Stabilities
… was developed using analytical thin-layer isoelectric focusing (IEF) on polyacrylamide gel (PAG) plates. A total of 18 (pI's 3.5-9.1) peroxidase isoenzymes were identified from crude extracts of pea seeds; all
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Cytological and Biochemical Studies of Male Sterility in Two Species of Drosophila
… examined by two-dimensional gel electrophoresis, isoelectric focusing and Western blotting to determine the biochemical basis for sterility. One protein, KRP60, was consistently reduced in sterile males. This protein may play a role in the normal function of the sperm in early development.
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Two-dimensional analysis and characterization of milk proteins
… in which the first dimension is preparative isoelectric focusing (IEF) and the second dimension is analytical gel electrophoresis was developed. The analysis of caseins and skim milk showed good separation between $\alpha$s1- and $\alpha$s2-caseins, and two separate bands in $kappa$-casein …
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Some properties and function of ribosomal proteins from the seeds of Pinus lambertiana
… were compared with normal ribosomes; the isoelectric focusing patterns of proteins removed (supernatant fraction) during washing procedures and those of proteins remaining (ribosomal pellet fraction) were investigated; characteristics of extracted r-proteins were examined. Ribosomal …
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Multidimensional Protein Separations in a Plastic Microfluidic Network
… reduce sample consumption and dead volume, an isoelectric focusing separation and dynamic sample introduction are demonstrated in a microfluidic microchannel. The dynamic sample introduction in plastic microfluidic devices can be directly controlled by various electrokinetic conditions, …
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Investigation of purification procedures to isolate rat mitochondrial ẟ - aminolaevulinic acid synthetase
… of affinity chromatography were unsuccessful. 4. Isoelectric focusing gave no clearly useful or preparative separations of ALV-Synthetase in pH gradients.
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DEVELOPMENT OF A NEW SCREENING AND DETECTION METHOD FOR IDENTIFYING PROTEIN-SMALL MOLECULE INTERACTIONS
… our laboratory, a new technique termed Dynamic Isoelectric focusing Anisotropy Binding Ligand Assay (DIABLA) is being in collaboration with the Tolley Laboratory (SIU) developed to fulfill this task. In this technique, a protein mixture is separated within the capillary using dynamic isoelectric …
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APPLICATIONS OF DYNAMIC ISOELECTRIC/ANISOTROPY BINDING LIGAND ASSAY FOR PROTEOMIC RESEARCH
… protein separation techniques. Dynamic isoelectric focusing (DIEF) is new technique similar to capillary isoelectric focusing (CIEF) invented by Dr. Luke Tolley at Southern Illinois University Carbondale. Using DIEF, the electric field inside the separation capillary can be modified …
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Studies on bovine γ-glutamylamine cyclotransferase.
… on Mono-Q under reducing conditions, isoelectric focusing of the alkylated sample, electroelution, electrophoresis, ultrafiltration, and lyophilization. The enzyme was purified more than 2,000 fold to a specific activity of more than 1,300U/mg of enzyme. A monomeric enzyme of molecular …
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ΑΠΟΜΟΝΩΣΗ ΚΑΙ ΧΑΡΑΚΤΗΡΙΣΜΟΣ ΤΗΣ ΚΙΝΑΣΗΣ ΚΑΖΕΙΝΗΣ ΙΙ ΑΠΟ ΣΠΛΗΝΑ ΠΑΙΔΙΩΝ ΜΕ ΜΕΣΟΓΕΙΑΚΗ ΑΝΑΙΜΙΑ
… SDS POLYACRYLAMIDE GEL ELECTROPHORESIS AND ISOELECTRIC FOCUSING HAVE PROVED THAT THE ENZYME WAS HIGHLY PURIFIED. THE PURIFICATION STEPS INCLUDED ION EXCHANGE CHROMATOGRAPHY, AMMONIUM SULFATE FRACTIONATION AND MOLECULAR SIEVING. THE PURIFIED ENZYME PHOSPHORYLATES IN PREFERENCE ACIDIC …
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ΣΥΜΒΟΛΗ ΣΤΗ ΜΕΛΕΤΗ ΤΗΣ ΑΝΤΟΧΗΣ ΤΩΝ ΨΕΥΔΟΜΟΝΑΔΩΝ ΣΤΑ ΑΝΤΙΒΙΟΤΙΚΑ
… TYPE OF ENZYME PRODUCED WAS DETERMINED BY THE ISOELECTRIC FOCUSING METHOD. (SHORTENED)
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On-chip multi-dimensional biomolecule separation using multi-layer microfabricated valves
… a microfluidic channel. The new methods couple isoelectric focusing (IEF) with high ionic strength capillary electrophoresis (CE) by active micro valve control in a microchip. Several experiments demonstrating independent 2D separation were performed, and critical parameters for better chip …
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Proteolysis of mammary secretion proteins during involution of bovine mammary gland
… the dry period, were analyzed by preparative isoelectric focusing, followed by SDS-PAGE. Protein profiles changed throughout the dry period. Intact casein bands were present throughout the dry period, but in reduced proportions from d 7 after drying-off through d 7 prepartum. Breakdown …
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Detection of Unique Tumor-Associated Proteins in the Urine of Patients with Transitional Cell Carcinoma by High Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis
… - dimensional electrophoretic technique using isoelectric focusing in the first dimension and sodium dodecylsulfate - polyacrylamide gel electrophoresis in the second dimension. Ten transitional cell carcinoma patients, ten renal cell carcinoma patients, two benign bladder patients, two benign …
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Genetic Studies on Lipoxygenase and Some Other Factors Possibly Associated With Soybean Oil Stability
… measurement of oxygen uptake, electrophoresis, isoelectric focusing and immunochemical studies all confirm the lack of lipoxygenase-1 (L-1) in these two genotypes. The lack of L-1 was found to hold throughout seed development and the first seven days of germination. No soybean genotypes were …
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