Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"irreversible inactivation"”.
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Halo enol lactone and protio enol lactone inhibitors of alpha-chymotrypsin: Mechanism and stereospecific behavior of inhibition
A kinetic study of inactivation of $\alpha$-chymotrypsin by enol lactones was performed. Halo enol lactones, 3-(1-naphthyl)-6(E)-(iodomethylene)tetrahydro-2-pyranone ($\alpha$Np6I) and 3-phenyl-6(E)-(bromomethylene)tetrahydro-2-pyranone ($\alpha$Ph6Br), showed burst kinetics in the inactivation of …
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Understanding the Dynamics of Embryonic Stem Cell Differentiation
… study the dynamics and the factors affecting the irreversible exit from pluripotency. Expression of the reporter Rex1-GFPd2, which is inactivated upon exit from naive pluripotency, was analyzed by quantitative long-term single-cell imaging over many generations. This technique allowed chemical, …
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Evidence for a third thermal parameter of enzymes
… the catalytic reaction defined by ΔG‡cat, and irreversible inactivation defined by ΔG‡inact. To account for the anomalies found in the variation of enzyme activity with temperature, a new model (the 'Equilibrium Model') has been formulated to describe the effect of temperature on enzyme …
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Blockade of Acetylcholine Receptors by Cobra Toxin Electrophysiology and Pharmacology
… with first-order kinetics. The rate constant for inactivation increased linearly with the toxin concentration near the receptors. Micro-ionophoretic application of two reversible agonists, ACh and carbamylcholine (Carb), focally protected receptors against bath-applied toxin. Bath-applied Carb and …
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Stability of microbial transglutaminase and its reactions with individual caseins under atmospheric and high pressure
Kinetic inactivation of factor XIIIa and MTG were performed in a pressure range from 0.1 to 400 MPa at 40°C within a time from 0 to 60 min in a TRIS-acetate buffer at pH 6.0. The inactivation of both enzymes at these conditions followed a first order reaction model. The high inactivation rate …