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Showing 1 to 16 of 16 for “"ion mobility mass spectrometry"”.
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Utility of Ion Mobility – Mass Spectrometry in Peptidoglycan Structural Determination
… time commitment. Potential isomers, modifications, and cross-linking patterns within whole cell digests lead to complex samples that typically require manual analysis. The introduction of a method for streamlining the identification of peptidoglycan components (muropeptides) is needed to …
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The application of ion mobility mass spectrometry to molecules of pharmaceutical significance
Ion mobility-mass spectrometry experiments have been conducted to measure the drift-time and calculate collision cross-sections (CCSs) using travelling wave ion mobility spectrometry, and determine the CCS using drift-tube ion mobility spectrometry systems of analytes. The aim of the study was to …
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Applications of Ion Mobility Mass Spectrometry - Screening for SUMOylation and Other Post-Translational Modifications
<p>Post-translational modifications (PTMs) of proteins are fundamental processes that trigger, regulate and terminate most of the cellular mechanisms by covalent attachment of chemical moieties to substrate proteins. Conjugation to the Small Ubiquitin-like MOdifier (SUMO) is a highly conserved and …
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Development of ion mobility mass spectrometry deconvolution strategies for use in data-independent acquisition proteomics.
Modern mass spectrometers are among the most sensitive and suitable analytical tools for high-throughput protein characterization. However, limitations in ion mobility (IM) and mass spectrometry (MS) resolving powers can limit the success of MS-based proteomics approaches. This work highlights the …
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Development of Ion Mobility Mass Spectrometry Instrumentation to Investigate the Gas-Phase Structures of Protein and Protein Complex Ions
This dissertation reports the development of new ion mobility mass spectrometry (IM-MS) instrumentation to analyze protein and protein complex ions. IM-MS is a gas-phase analytical technique that separate ions based on their collision cross section (a description of ion shape) and mass-to-charge …
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Investigating structural and stability characteristics of native-like proteins using ion mobility-mass spectrometry (IM-MS) in positive- and negative-ion mode.
Native ion mobility-mass spectrometry (IMS-MS) is widely used to investigate the structure and stability of biologically relevant proteins and protein complexes. The key goal, and arguably biggest attraction, of native IMS-MS is maintaining the noncovalent interactions responsible for stabilizing …
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Synthesis and Physical Properties of Helical Nanosized Quinoline-based Foldamers: Structure, dynamics and photoinduced electron transport
Herein, synthesis, characterization and application (photoinduced electron transport) of nanosized quinoline-based foldamers have been explored. With double segment strategy, a variety of helical nanosized foldamers (up to 96 quinoline units) were successfully prepared based on …
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BINDING ENERGIES AND SOLVATION OF ORGANIC MOLECULAR IONS, REACTIONS OF TRANSITION METAL IONS WITH, AND PLASMA DISCHARGE IONIZATION OF MOLECULAR CLUSTERS
In this dissertation, different approaches have been employed to address the quest of understanding the formation and growth mechanisms of carbon-containing molecular ions with relevance to astrochemistry. Ion mobility mass spectrometry and DFT computations were used to investigate how a second …
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Ion Mobility and Gas-Phase Covalent Labeling Study of the Structure and Reactivity of Gaseous Ubiquitin Ions Electrosprayed from Aqueous and Denaturing Solutions
Gas-phase ion/ion covalent modification was coupled to ion mobility/mass spectrometry analysis to directly correlate the structure of gaseous ubiquitin to its solution structures with selective covalent structural probes. Collision cross-section (CCS) distributions were measured prior to ion/ion …
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Investigation of mass spectrometry approaches for aiding fragment-based drug discovery
The use of mass spectrometry (MS)-based techniques to study protein structure dynamics is a relatively new field, with seminal developments such as nanoelectrospray ionization (nano-ESI) and traveling-wave ion mobility-mass spectrometry (TWIM-MS) MS being introduced only around twenty-five and …
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Characterizing Conformational Dynamics and Catalytic Activity of Enzymes by Hydrogen-Deuterium Exchange Mass Spectrometry
Determining the conformational dynamics of enzymes as they undergo catalysis has been challenging for decades due to the short timescale and limitations of traditional analysis, including techniques such as CPMG 2D NMR and X-ray crystallography. Herein is the study of two proteins: TEM-1 …
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Native surface mass spectrometry via static liquid extraction and laser ablation sampling for structural characterization of macromolecules in gas phase.
Native Surface Mass Spectrometry is an emerging field in biochemical sciences that aims to combine non-denaturing macromolecular proteomics with structural biology and biophysics to elucidate protein functions. By sampling endogenous proteins from their intact biological matrix, proteins' …
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Proteomic Analysis of the Blood of <i>Alligator mississippiensis</i>
… focus was to investigate alligator blood using mass spectrometry-based proteomics methods to understand their innate immune systems. The first goal was to sequence peptides and proteins from the blood serum and leukocytes using tandem mass spectrometry and <i>de novo</i> sequencing. The second …
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GROWTH MECHANISMS OF COMPLEX ORGANICS IN THE GAS PHASE AND ON METAL NANOPARTICLES GENERATED BY A LASER VAPORIZATION PROCESS
In this dissertation, the ion mobility mass spectrometry technique is used to study the possible mechanisms of formation of polyaromatic hydrocarbons (PAHs) and polyaromatic nitrogen-containing hydrocarbons (PANHs) via the ion-molecule reactions of acetylene neutrals with different aromatic …
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Progettazione, sintesi e caratterizzazione di sistemi biomimetici e molecole ligandi di ioni metallici come agenti teranostici.
L'inibizione del processo di auto-aggregazione dei peptidi monomerici di amiloide-β (Aβ) è un approccio chiave nello sviluppo di farmaci per la malattia di Alzheimer (AD), in cui oligomeri a basso peso molecolare (LMW), solubili e altamente neurotossici, sono prodotti e aggregano nel cervello nel …
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Gas Phase Studies of Molecular Clusters Containing Metal Cations, and the Ion Mobility of Styrene Oligomers
… the mechanism of the self-initiated polymerization (or thermal polymerization) of styrene in the gas phase. In this work, we present the first direct evidence for the thermally self-initiated polymerization of styrene in the gas phase. Our approach is based on on-line analysis of the gas phase …