Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"ion conductance"”.
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Two-Electrode Voltage Clamp (TEVC) Based Scanning Ion Conductance Microscopy: New Methodology and Validation
Scanning ion conductance microscopy (SICM) is a scanning probe technique that utilizes an electrolyte filled glass-pipette to recover the topography of a non-conductive sample in an electrolyte solution. This technique monitors the change in current that is caused by a change of resistance through …
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Localised Dosing and Nanodetection Using a Novel Scanning Ion Conductance Microscope and Its Application to Alzheimer's Disease
Scanning ion conductance microscopy (SICM) is a technique for non-contact topographic imaging. In this thesis, a biophysical investigation into Alzheimer's Disease (AD) was carried, with toxic oligomers dosed locally and quantitatively on to single astrocytes using SICM and simultaneously …
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Pharmacological Modulation of Cancer Migration and Invasion Through Targeting AQP1 Ion and Water Channel Activity
Cancer invasion and metastasis are the leading causes of cancer-related deaths. Aquaporin-1 is a dual water and ion channel that is upregulated in many aggressive cancers including colon, breast, and brain cancer; aquaporin-1 enhances cell migration, invasion and metastasis in these cancer types. …
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Molecular Separation and Power Generation by Carbon Nanomembranes
Mass transport in two-dimensional (2D) membranes has been at the forefront of materials research for nearly a decade. It is believed that 2D membranes can achieve high permeation and selectivity due to their nanometer-scale thickness and narrow pore size distribution. Herein, we focused on the …
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Anwendungen der Ionenleitfähigkeitsmikroskopie in der Biophysik
In dieser Arbeit wird ein kombiniertes Rasterionenleitfähigkeits-/Weitfeldfluoreszenzmikroskop entwickelt. Die Zusammenführung der verschiedenen Techniken erlaubt das Erforschen neuer, unkonventioneller Methoden in der Biophysik. Die Weitfeldfluoreszenzmikroskopie bietet in Kombination mit …
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Novel Fabrication and Characterization Methods for Conducting Polymer Nanostructures and Microstructures
… novel experimental techniques for the fabrication and characterization of poly(3,4-ethylenedioxythiophene) (PEDOT) and polyaniline (PANI), two prominent conducting polymers. Many of the strategies presented herein are based on miniaturized pipettes driven by scanning ion conductance microscopy …
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Nano-electro-mechanical systems fabricated by tip-based nanofabrication
This dissertation explores the use of a heated AFM tip for fabrication of NEMS devices. Two critical challenges hindering TBN from NEMS fabrication are addressed in this thesis. First, we experimentally found out that polystyrene nanopatterns deposited by a heated AFM tip can serve directly as etch …
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Less toxic yet still resistance evasive amphotericins and atomistic probing of the amphotericin B ion channel
… macrolide natural product with two major functions: antifungal activity and ion channel formation. For over 50 years AmB has remained the last line of defense against systemic fungal infections. Remarkably, AmB has evaded the development of drug resistance during this extensive clinical …
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Investigations into the Lipid Transport Mechanisms and Energetics of Bacterial ATP-Binding Cassette (ABC) Transporters MsbA and LmrA
… is its ability to harness electrochemical ion gradients. When measured in an electrophysiological setting, LmrA exhibits ion conductance upon the binding of ATP, which is based on a 2Na<sup>+</sup>/(1H<sup>+</sup>-1HEPES<sup>+</sup>-1Cl−)<sup>+</sup> exchange reaction. It also shows a …
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Small molecules that imperfectly replicate protein function of missing proteins restore physiology in yeast and human epithelia
… diseases caused by an excess of protein function can often be treated with small molecules that bind and inhibit the corresponding proteins. In contrast, there are many currently incurable human diseases that are caused by missing protein ion transporters, including cystic fibrosis and …
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Characterization of the Central Cavity of a Potassium Channel: Helix Dipoles, Conformational Plasticity and Inhibition
… important for regulating the flow of potassium ions across semi-permeable cell membranes in an efficient and selective manner. Potassium channels form a conduction pore comprised of a selectivity filter responsible for the strong preference for potassium, and a water-filled central cavity that …
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Computational modeling of peptide-mediated charge transport and membrane-associated phenomena
… by shuttling electrons through a series of reactions to fuel the synthesis of adenosine triphosphate (ATP). In mitochondria, membranes provide an ideal structural framework to efficiently carry out the electron transport that forms the basis of biological energy production processes. By relying …