Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 149 for “"introns"”.
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Discovery and characterization of stable introns in yeast
Spliceosomal introns are a defining feature of eukaryotes; they are present in all known eukaryotic genomes, absent from all known non-eukaryotic genomes, and their accurate removal is essential for mRNA maturation. Although smaller ncRNAs can be processed from introns, the introns themselves are …
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The evolution and specificity of RNA splicing
… rather punctuated by long interruptions known as introns. These introns are copied from generation to generation, and even from cell to cell as a person grows from an embryo into an adult. Each time a gene is activated, the cell must first accurately excise all the introns in a process known as …
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Sequence-Dependent & -Independent Effects of Intron-Mediated Enhancement (IME)
Introns are ubiquitous features of eukaryotic genes, and their precise removal from pre-mRNA transcripts by the spliceosome is an essential step in gene expression. Genomic deletion of an intron from a gene tends to reduce its expression, and addition of an intron tends to increase it. This …
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Demarcation of coding and non-coding regions of DNA using linear transforms
… or the regions that code for amino acid, and the introns, or the regions that do not code for amino acid. The main aim of the thesis is to study signal processing techniques that help distinguish between the regions of the exons and the introns. Previous research has shown the fact that the exons …
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Enzymatic and Splicing Activity of Class I Leucyl -Trna Synthetases From Escherichia Coli and Saccharomyces Cerevisiae
… for splicing of the mitochondrial bI4 and al4a introns. Splicing of these introns is dependent on coordination between ymLeuRS and bI4 maturase. An in vitro splicing assay has been established that is based on assembly of proteins into a competent ternary splicing complex. RNA deletion mutants …
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An investigation of genetic variation within northwest Atlantic Porphyra (Bangiales, Rhodophyta) with specific phylogeographic analysis of the common, rocky intertidal species, Porphyra umbilicalis
… presence and variability of group-I introns of the ribosomal small subunit (SSU) were screened in North Atlantic species of Porphyra in order to assess whether they could be biogeographically informative. In an initial screening for the helix 50 intron, using flanking primers with the …
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Transcript Diversity In The Protozoan Parasite Toxoplasma Gondii
… ~8300 protein-coding genes, fragmented by ~40K introns. Untranslated regions are incompletely defined, few alternatively-spliced transcripts are described, and non-coding transcripts remain largely unexplored. mRNA-seq datasets presented in this dissertation define a total of 2.7M introns, most …
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Mobile genetic elements in Coxiella burnetii: Friends, foes or just indifferent?
… of mobile genetic elements including two group I introns and an intervening sequence (IVS) that interrupt the 23S rRNA gene; an intein within dnaB (encoding replicative DNA helicase) and a homing endonuclease. The introns are self-splicing ribozymes and able to inhibit ribosome function and retard …
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Mobile genetic elements in Coxiella burnetii: Friends, foes or just indifferent?
… of mobile genetic elements including two group I introns and an intervening sequence (IVS) that interrupt the 23S rRNA gene; an intein within dnaB (encoding replicative DNA helicase) and a homing endonuclease. The introns are self-splicing ribozymes and able to inhibit ribosome function and retard …
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Taxonomy and Symbiosis in Associations of Physciaceae and Trebouxia
… nrSSU-Gene der Physciaceen enthielten mehr Introns als die aller anderen bekannter Organismengruppen. Der einzigartige Datensatz konnte genutzt werden, um konservierte Regionen innerhalb dieser Introns zu identifizieren. Auf diese konservierten Regionen konnten Primer konstruiert werden, die …
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Characterization of splicing mechanisms by single-molecule fluorescence
<p>Group II introns rank amongst the largest self-splicing ribozymes found in bacteria and organellar genomes of various eukaryotes. Despite the diversity in primary sequences, group II introns posses highly conserved secondary structures consisting of six domains (D1-D6). To perform its function, …
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Modulation de l'épissage alternatif de hnRNP A1 : éléments de contrôle et mécanismes d'action
… propriété de moduler l'épissage alternatif. Les introns flanquant l'exon alternatif contiennent plusieurs régions conservées entre l'homme et la souris. Leur identification a permis de mettre en évidence des éléments impliqués dans le contôle [i.e. contrôle] de l'épissage de l'exon 7B et a mené à …
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Identification of novel branch points reveals insights into RNA processing
… number of novel BPs both in annotated introns and other genomic regions. We attempted to verify these novel introns using RNA-seq and Lariat-seq and surprisingly observed considerable amounts of alternative splicing (AS) in S. cerevisiae beyond the previously known stress-regulated …
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Methyl Chloride Transferase: Isolation, characterization,cDNA Cloning, and Expression
… characterized and is composed of 8 exons and 7 introns.
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Computational methods for splice site prediction
… genes consist predominantly of exons and introns; while the introns are non-coding regions that are removed from the primary transcript during RNA splicing, the exons are the coding regions that are spliced together during the mRNA maturation process. The borders between exons and introns …
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Interaction of the Neurospora crassa mitochondrial tyrosyl-tRNA synthetase with group I intron RNAs
… or CYT-18 protein, functions in splicing group I introns by promoting the formation of the catalytically active structure of the intron RNA. To investigate how CYT-18 stabilizes the active RNA structure, I used an Escherichia coli genetic assay with the phage T4 td intron to systematically test …
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Diversity, Distribution and Evolution of the Planktonic Diatom Family Chaetocerotaceae
… Several rDNA sequences contained spliceosomal introns (ca. 100bp) and/or group-I introns (ca. 400bp). Phylogenies inferred from the introns did not corroborate rDNA phylogenies, suggesting horizontal gene transfer. Presence/absence of introns in conspecific strains sampled in different seasons …
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Molecular analysis of wheat snRNAs
Pre-mRNA splicing is a complex event in which introns are removed and exons spliced together. This process is mediated by small ribonucleoprotein particles (snRNPs), formed from small nuclear RNAs (snRNAs) and their associated nuclear proteins.
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Structure and expression of a U1 snRNA gene from Arabidopsis thaliana
… snRNAs that function in the removal of introns from pre-mRNA. U1 snRNA is encoded by a small multigene family in Arabidopsis, consisting of between five and eight U1 genes. U1a snRNA is unique in having an extra U residue at position +2, which occurs in the region of U1 RNA which base …
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