Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"intronless"”.
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Epigenetic repression of intronless mobile elements by the HUSH complex
… repress a broad range of sequence-diverse, intronless invading DNAs, despite no prior exposure to such DNAs. Sequence length and high adenine (A) content in the sense strand are important additional determinants of susceptibility to HUSH-targeting. My further work demonstrated that …
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Characterization in cochlea of KCTD12/PFET1, an intronless gene with predominant fetal expression
… KCTD12/Kctd]2 is an evolutionarily conserved intronless gene encoding a 6 kb transcript in human and three transcripts of approximately 4, 4.5 and 6 kb in mouse. The protein, pfetin, is predicted to contain a voltage-gated potassium channel tetramerization (T1) domain. This thesis reports …
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Investigating Mechanisms of Nuclear mRNA Export
… it remains unclear whether RNAs generated from intronless genes use specific machinery for their export. We show that TPR is required for promoting the efficient export of intronless or intron-poor mRNAs and lncRNAs. Furthermore, we observe a strong correlation between transcripts generated from …
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Molecular cloning and characterization of two Arabidopsis thaliana sulfotransferases
… database using AtST1 as a query sequence. The intronless AtST4a was cloned via PCR from Arabidopsis thaliana ecotype Col-0 genomic DNA. A cDNA clone of AtST6 was obtained and fully sequenced. AtST4a and AtST6 were both cloned into the bacterial expression vector pQE30. The recombinant proteins …
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Co-transcriptional recruitment of the U1 snRNP
… introns but not within promoter regions or along intronless genes. More specifically, accumulation correlated with the presence and position of the intron, indicating that the intron is necessary for co-transcriptional U1 snRNP recruitment and/or retention (Kotovic et al., 2003). In contrast to …
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Characterization of cDNA and Genomic Clones for a Palmitoyl-acyl Carrier Protein Thioesterase and an Osmotin-Like PR5 Protein in Gossypium Hirsutum.
… seen in thaumatin, zeamatin, and PR5-d. The intronless cognate cotton genomic clone has two putative ethylene response elements (GCC boxes) found in other PR5 gene promoter regions, as well as several tentative promoter/enhancer elements possibly involved in spatial/temporal gene expression.
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Rapid Expansion and Specialization of the Bitter Taste Receptor Family in Amphibians
… bitterness is detected with a family of intronless G protein-coupled receptors called TAS2Rs. TAS2Rs are well studied in the human and mouse mouth, but recent studies have explored additional species and reported extra-oral expression of a subset of receptors. Here, we systematically …
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Sequence-Dependent & -Independent Effects of Intron-Mediated Enhancement (IME)
… increase in both mRNA and protein output over intronless controls, suggesting that the enhancement is largely at the level of mRNA accumulation. We analyze the sequence features associated with highly-enhancing introns and demonstrate that the poly-uridine (polyU) content of an intron is …
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Graužikų naujų poliomos virusų koduojamų baltymų sintezės bei jų funkcijos tyrimas bakterijų ir mielių ląstelėse /
… Application of molecular tools reveals that the intronless LTAg, LTAg and STAg genes are successfully transcribed in yeast cells and inefficient transcript splicing of the LTAg gene occurs. Further characterisation of unknown function viral proteins requires the identification of protein domains …
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Characterization of the Interactions Between Kaposi’s Sarcoma-Associated Herpesvirus ORF57 and Its RNA Partners
… were also inserted into the 3' UTR of an intronless β-globin reporter to test ORF57 responsiveness in vivo. Our analyses revealed an ORF57 responsive element (ORE) at the 5' end of PAN RNA that we hypothesize functions as a high-affinity binding site to recruit ORF57. Next, we optimized …
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Characterization of Chitinase Activity and Gene Expression in Muskmelon Seeds
… two introns in the coding region while Cmchi1 is intronless. Cmchi1 cDNA encodes a class III chitinase while Cmchi2 cDNA encodes a class II chitinase. Cmchi1 and Cmchi2 proteins might be targeted to secretory pathways because they possess signal peptides. Southern blotting suggested that there is …
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Analysis and application of the En transposable element and genetic study of resistance to Bipolaris maydis in maize
… region, in the coding sequence of the intronless A2 gene. In the second project, at least one En-mutable allele has been relocated to each of the 20 maize chromosome arms. Because En has been shown to transpose more frequently to closely linked sites, the relocation of En elements to …
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<i>Plasmodium</i>-Induced Nitrosative Stress in <i>Anopheles stephensi</i>: The Cost of Host Defense
… epithelium and is encoded by a single-copy, intronless gene. Quantitative RT-PCR analyses confirmed that induction of <i>AsPrx</i> expression in the midgut was correlated with malaria parasite infection and nitrosative stress. To determine whether AsPrx could protect against RNI- and …
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The role of human and Drosophila NXF proteins in nuclear mRNA export
… shock mRNAs, including intron-containing and intronless mRNAs, was inhibited. Depleting endogenous NXF2 or NXF3 had no apparent phenotype. These results suggested that NXF1 (but not NXF2-NXF4) mediates the export of bulk mRNA in Drosophila cells. We and others have shown that human NXF …