Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 304 for “"intron"”.
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Intron Retention Induced Neoantigen as Biomarkers in Diseases
… aberrant peptides derived from unspliced introns can be presented by the major histocompatibility complex (MHC) class I molecules on the cell surface and elicit immunogenicity. In this dissertation, we first developed an integrated computational pipeline for identifying IR-induced …
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Artificial Intron Technology to Generate Conditional Knock-Out Mice
… of targeted genes that uses a small standardized intron that should be easily introduced using CRISPR/Cas9 methodology. I tested the DECAI (<strong>DE</strong>gradation based on <strong>C</strong>re-regulated- <strong>A</strong>rtificial <strong>I</strong>ntron) artificial intron technology, …
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Sequence-Dependent & -Independent Effects of Intron-Mediated Enhancement (IME)
Introns are ubiquitous features of eukaryotic genes, and their precise removal from pre-mRNA transcripts by the spliceosome is an essential step in gene expression. Genomic deletion of an intron from a gene tends to reduce its expression, and addition of an intron tends to increase it. This …
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Mechanisms of leucyl-tRNA synthetase dependent group I intron splicing
… with a maturase to excise the bI4 and aI4α introns from the cob and cox1α genes respectively. The LeuRS-based suppressor mutations have been isolated within the amino acid editing CP1 domain and restore native RNA splicing activity in the presence of an inactive maturase. Mutational analysis …
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DNA target site recognition by the Ll.LtrB group II intron RNP
Mobile group II introns are retroelements that site-specifically insert into DNA target sequences. The group II intron mobility pathway is mediated by a ribonucleoprotein particle (RNP) composed of excised intron RNA and an intron-encoded protein (IEP). The intron lariat inserts at a specific DNA …
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Elucidation of the kinetic folding pathway of a Group I intron
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1996.
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Characterization and Functional Analysis of the Arabidopsis Intron Splicing Factor ASF/SF2
ASF/SF2 protein is first time shown to be a potential substrate for the mitotic cdc2/cyclin kinase. UV crosslinkage assay demonstrate RNA binding ability of SF2a and RRM domain, but not SF2b protein. Immunostaining of TN-5 cells expressing recombinant proteins and western blot analyzing cytoplasmic …
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Role of the Intron 13 Polypyrimidine Tract in Soluble Flt-1 Expression
… activity. This project focuses on characterizing intronic elements that regulate Flt-1 mRNA splicing. A "wild-type" construct (pFIN13), containing the first 13 exons, intron 13 and exons 14-30 of mouse Flt-1, was shown to produce both forms of Flt-1 mRNA after transfection into HEK293 cells. To …
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Rethinking phylogenetics using Caryophyllales (angiosperms), matK gene and trnK intron as experimental platform
… We also investigate the utility of trnK intron in phylogeny reconstruction at relativley deep evolutionary history (the caryophyllid order) by comparing it with rapidly evolving matK. We show that trnK intron is comparable to matK in terms of the proportion of variable sites, parsimony …
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Interaction of the Neurospora crassa mitochondrial tyrosyl-tRNA synthetase with group I intron RNAs
… or CYT-18 protein, functions in splicing group I introns by promoting the formation of the catalytically active structure of the intron RNA. To investigate how CYT-18 stabilizes the active RNA structure, I used an Escherichia coli genetic assay with the phage T4 td intron to systematically test …
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Insights into the rice and Arabidopsis genomes: intron fates, paralogs, and lineage-specific genes
… we examined</p> <p>segmental duplication, intron turnover, and paralogous protein family</p> <p>composition in rice. These data suggest a large percentage of the rice genome</p> <p>was involved in segmental duplication creating a large number of paralogous</p> <p>families. We found that …
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Prevalence and frequency spectra of single nucleotide polymorphisms at exon-intron junctions of human genes
In humans and other higher eukaryotes the observation of multiple splice isoforms for a given gene is common. However it is not clear whether all of these alternatively spliced isoforms are a product of true alternative splicing or some are due to DNA sequence variations in human populations. …
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Microbiology of periodontal disease in Singapore and the discovery of a bacterial intron in Actinobacillus actinomycetemcomitans
Ethnicity is a risk factor for periodontal disease. The occurrence of major periodontal pathogens in a total of 410 periodontally healthy and diseased subjects in the ethnic Chinese in Singapore were determined. Actinobacillus actinomycetemcomitans cdt; Porphyromonas gingivalis prtC and Tannerella …
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Characterization of the Group II Intron Gs. Int1 from the Thermophilic Bacterium <em>Geobacillus stearothermophilus</em>.
<p>Group II Introns are small segments of DNA that reside in the chromosome of bacteria or the organelles of primitive eukaryotes. These elements have some very interesting properties. First, they are retrotransposons that can move from one location to a new location in DNA via a reverse …
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Fluorescence Investigations of RNA Folding: Group I Intron P4-P6 Domain and Xpt G -Riboswitch Aptamer Domain
… P4-P6 domain of the Tetrahymena group I intron RNA, xpt G-riboswitch aptamer domain, FRET.
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In Vivo Studies of Yeast Mitochondrial Intron Splicing : Ectopic Branching and a Screen for Nuclear Encoded Splicing Factors
The splicing mechanism of group II introns is analogous to that of nuclear introns and it is generally thought that both share a common ancestor. This work contains two studies of group II intron splicing in yeast mitochondria. Previous studies done in collaboration with Dr. Anna Pyle at Yale …
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NOVEL THERAPEUTIC STRATEGIES IN LUNG CANCERSTRONG BIOLOGICAL BIAS FOR ALK INTRON 19 BREAKPOINTS IN NON-SMALL LUNG CANCER
… in the EML4 gene, most frequently located in intron 6 or 13, ALK breakpoint is almost invariably in intron 19. Rare reports describe EML4-ALK translocations with breakpoints in intron 17 or intron 18 of the ALK gene. Despite all these ALK breakpoints potentially generate oncogenic fusions, the …
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Interaction between positive- and negative- regulatory elements in the first intron controls transcription of the glutamine synthetase gene during adipocyte differentiation
… regions of the mouse GS gene in the first intron and the distal 5'-flanking sequence. These two elements function cooperatively to regulate GS transcription during adipocyte differentiation. In my studies, I identified a putative 32 bp enhancer as well as a closely associated silencer in …
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An investigation of genetic variation within northwest Atlantic Porphyra (Bangiales, Rhodophyta) with specific phylogeographic analysis of the common, rocky intertidal species, Porphyra umbilicalis
… presence and variability of group-I introns of the ribosomal small subunit (SSU) were screened in North Atlantic species of Porphyra in order to assess whether they could be biogeographically informative. In an initial screening for the helix 50 intron, using flanking primers with the …
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