Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"intrinsically disordered regions (IDRs)"”.
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Characterization of the Roles of Intrinsically Disordered Regions from RNA-Binding Proteins in Phase Separation
… enrich RNA and RNA-binding proteins containing intrinsically disordered regions (IDRs). I demonstrate that IDRs, coupled with RNA binding domain and RNA, can phase separate in vitro, producing dynamic liquid droplets. Over time, these droplets mature into more stable states, as assessed by …
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Biophysical mechanisms of intrinsic protein disorder: lessons from a protein backbone model
Intrinsically disordered regions (IDRs) in proteins populate a dynamic, heterogenous ensemble of structures. Proteins involved in the regulation of critical cellular functions rely on IDRs to carry out their functions. IDRs have been implicated in a number of diseases for which there are currently …
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Molecular modelling of Androglobin
… algorithms with the aims of identifying intrinsically disordered regions (IDRs), that may not only add noise when subjecting the sequence to fold recognition techniques. These IDRs may also be in close proximity to key molecular recognition features (MoRFs). Secondary structure prediction …
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Sequence-Based Properties that Identify Intrinsically Disordered Phase-Separating Protein Regions
… drive liquid-liquid phase separation (LLPS) of intrinsically disordered proteins (IDPs). This phenomenon is critical in many cellular processes (including RNA metabolism, chromatin rearrangement, and signal transduction), and known to be driven primarily, but not exclusively, by IDPs. To fully …
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Primary Structure and Solution Conditions Determine Conformational Ensemble Properties of Intrinsically Disordered Proteins
<p>Intrinsically disordered proteins (IDPs) are a class of proteins that do not exhibit well-defined three-dimensional structures. The absence of structure is intrinsic to their amino acid sequences, which are characterized by low hydrophobicity and high net charge per residue compared to folded …
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Biomolecular condensation of intrinsically disordered regions in P. falciparum and H. sapiens transcription factors
… control. Biomolecular condensation, driven by intrinsically disordered regions (IDRs) in proteins, has emerged as a previously underappreciated mechanism of cellular compartmentalisation and regulation. The study of eukaryotic transcription has been revolutionised by the understanding that …
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Investigating the regulation of processing bodies in Drosophila melanogaster oocytes
… assembly. At the molecular level, although the intrinsically disordered regions (IDRs) of a core P body protein are dispensable for condensate assembly, they are critical for maintaining P body integrity in the mature oocytes. In addition, RNA is also found to be essential for sustaining P body …
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Mechanical Manipulation of Eukaryotic Chromatin by DNA-Binding Proteins
… to other reported cellular forces, 2) the intrinsically disordered regions (IDRs) are dispensable for condensate formation but necessary for high force generation, and lastly, 3) chromatin components, such as nucleosomes and linker histone H1, attenuate the force generating capacity of Sox2 …
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New experimental and theoretical tools for studying protein systems with elements of structural disorder
<p>Disordered proteins are one class of proteins which do not possess well-folded three-dimensional structures as their native conformations. Many eukaryotic proteins have been found to be fully disordered or contain certain disordered regions. Disordered proteins usually display several …
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Paraspeckle protein NONO regulates active chromatin by allosterically stimulating NSD1
… regulation. Due to its size and extensive intrinsically disordered regions (IDRs), NSD1 has been difficult to purify and characterize. In the first study, I developed a baculovirus-insect cell system for expressing full-length (FL) NSD1 and SETD2. By isolating monoclonal baculovirus clones …
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Uncovering the Roles and Evolved Sequence Grammar of Hypervariable Intrinsically Disordered Proteins in Bacterial Cell Division
… core. FtsZ has a bristled architecture, where a disordered tail, called the C-terminal tail (CTT), flanks the folded domain. The essentiality of the CTT was established through deletion experiments; however, the exact role that the CTT plays within the context of FtsZ function remained unclear. …