Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"intrinsically disordered protein"”.
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Intrinsically Disordered Protein Polymer Libraries as Tools to Understand Protein Hydrophobicity
<p>Intrinsically disordered protein polymers (IDPPs) are repetitive biopolymers that, when enriched with prolines, glycines, and aliphatic amino acids, have observable lower critical solution temperature (LCST) phase transition behavior at physiologically relevant temperature and concentration …
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Mechanisms of Adsorption and Surface-Mediated Aggregation of Intrinsically Disordered Protein Tau at Model Surfaces
The adsorption and aggregation of an intrinsically disordered soluble protein, tau, into insoluble filaments is a defining hallmark of many neurodegenerative diseases, commonly referred to as tauopathies. In its native state, the protein tau’s function is to promote the assembly, and aid in the …
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Elucidating the Structural and Dynamical Properties of the Intrinsically Disordered Protein Nrf2 Using Molecular Dynamics Simulations
… factor erythroid 2-related factor 2 (Nrf2) protein is a critical transcription factor for activating the antioxidant response pathway, a primary defense mechanism against disproportionate levels of oxidants in the cell, via the upregulation of cytoprotective genes. Notably, aberrant …
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Direct Computations of Spatially Resolved Viscoelastic Moduli of Biomolecular Condensates
… models and simulations of A1-LCD, a type of intrinsically disordered protein, we examine how network topology influences storage modulus, loss modulus, crossover frequency, and relaxation time spectra. Our results reveal a strong correlation between topological features and mechanical …
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Insights into p53-Dependent Apoptotic Signaling and Cell Fate vis-a-vis Functional Cooperation among BCL-xL, Cytoplasmic p53, and PUMA
… upregulated modulator of apoptosis), a BH3‑only protein that binds and inhibits the anti‑apoptotic BCL‑2 repertoire, including BCL‑xL. Structural investigations of PUMA and the BCL‑xL×PUMA BH3 domain complex by X‑ray crystallography and nuclear magnetic resonance (NMR) spectroscopy reveal a …
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Modeling intrinsically disordered proteins ; a comprehensive study of [alpha]-synuclein
… challenging to study because it is an Intrinsically Disordered Protein (IDP); i.e., it lacks a well-defined structure in aqueous solution. Unlike folded proteins, IDPs typically interconvert between many different conformations during their biological lifetime. In this thesis we apply …
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The ultrastructural morphology of Eimeria tenella exhibits striking natural variation and undergoes significant changes during the first few hours of infection
… merger shares striking similarity with that of intrinsically disordered protein-containing droplet organelles; this comparison may help to direct future research into the character and function of these mysterious organelles. In an adjacent project, the organelle numbers and volumes for the E. …
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Developing Fast and Accurate Water Models for Atomistic Molecular Dynamics Simulations
… including those of biomolecules such as proteins and DNA. Despite water's simple structure and countless studies carried out over the decades, the best water models are still far from perfect. Water models are normally divided into two types--explicit model and implicit model. Here my …
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Exploring induced secondary structure and unmethylated DNA binding domains of methyl CpG binding protein 2 (MeCP2)
Our understanding of Methyl CpG binding protein 2 (MeCP2) structure and function has changed and expanded considerably over the last two decades. Mutations along the entirety of the human MeCP2 gene product lead to a disease state - Rett syndrome. The clinical connection of this protein has …
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Molecular Mechanisms of DNA Protection by Tardigrade Damage Suppressor Protein (Dsup): Structural Adaptation and Functional Interactions
… largely attributed to the DNA damage suppressor protein (Dsup). This study investigates the molecular mechanisms underlying Dsup’s DNA-protective function, focusing on its structural adaptability and interaction with nucleic acids. </p> <p>Using a combination of bioinformatics, biophysical …
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Structural and functional studies of intrinsically disordered fibronectin-binding proteins
Bacterial fibronectin-binding proteins (FnBPs) mediate adhesion of bacteria to host tissues through binding to the human protein fibronectin (Fn). FnBPs are predicted to contain a series of intrinsically disordered Fn-binding repeats (FnBRs), which undergo a disorder-to-order transition on binding …
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Yeast Ataxin-2 (Pbp1) Condensates Regulate TORC1 Activity and Autophagy in Response to Cellular Redox State
… ataxin-2, also known as Pbp1 (Poly(A) binding protein-binding protein 1), is an intrinsically disordered protein that has earlier been implicated in stress granule formation, RNA biology, and neurodegenerative disease. However, the normal endogenous function of Pbp1 and ataxin-2 remains poorly …
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Structural Biology in Cellular Environments Using Sensitivity Enhanced NMR
… state NMR to mammalian cells. DNP NMR can detect proteins at their physiological concentrations within cellular environment in tractable times. However, these experiments were limited to cellular lysates, which did not recapitulate the intracellular environment completely. Studying proteins within …
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Metabolism of Alpha-Synuclein by the 20S Proteasome
… by the intracellular aggregation of the neuronal protein alpha-synuclein (alphaSyn) that normally associates with synaptic vesicles. Within the aggregates, a fraction of alphaSyn is truncated at the C-termini, and these truncations are hypothesized to participate in disease pathogenesis. The …
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Understanding the membrane biophysics of alpha-Synuclein and its role in membrane curvature induction and structural remodeling
Alpha-Synuclein (aSyn) is a 140 amino acid, intrinsically disordered protein that adopts an extended amphipathic alpha-helical structure upon binding the membrane. aSyn is the major proteinaceous component of insoluble fibrillar Lewy bodies, a hallmark of Parkinson's disease (PD). The precise roles …
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Examining Post-Translational Modifications In Alpha-Synuclein Through Semi-Synthesis And Single Molecule Spectroscopy
Alpha-synuclein (αS) is an intrinsically disordered protein believed to mediate synaptic vesicle trafficking through interactions with lipid surfaces. The pathological, fibrillar aggregates of αS are implicated in a class of neurodegenerative disorders including Parkinson’s disease. A variety of …
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Programmable Assembly of Elastin-like Polypeptides Within Droplet Microenvironments
<p>Disordered proteins lack a defined secondary and higher order structure, possess a large number of biological functions, and are prevalent throughout nature. One important property of certain disordered proteins in biology is an ability to phase separate within the interior of a cell and form …
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Computer Simulation of Biological Systems
… surface; (b) the dynamical modelling of protein tau, an intrinsically disordered protein. We briefly describe here the two problems; for their detailed introduction we refer respectively to chapter DNA and chapter TAU. The interest in the study of the adsorption of DNA on functionalized …
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DEVELOPMENT OF MOLECULAR PROBES ACTING ON aSYN/TUBULIN AND TAU/TUBULIN INTERACTION
… locus, containing the gene coding for the Tau protein, a microtubule-binding protein that is predominantly expressed in neurons. The evidence of Tau pathology in PD is striking and contributes to neuronal dysfunction, impairment of axonal transport and cell death underlying PD. Recently another …
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Characterization of Oxidized Tau Protein Aggregation and Inhibition of Tau Aggregation via MAP2
… (NFT) composed of the microtubule stabilizing protein Tau are a pathological hallmark of Alzheimer's disease (AD) and many other dementia related diseases specified as Tauopathies. Due to alternative mRNA splicing, intrinsically disordered Tau exists as six separate isoforms in the adult human …
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