Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"intrinsic fluorescence"”.
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Monitoring glycation through the intrinsic fluorescence of biological fluorophores
… in some way. This work aims to establish if the intrinsic fluorescence of these compounds could be used to monitor the impact of glucose, and thus offer an alternative method to monitoring long term glycaemic control. We have studied the evolution of the intrinsic fluorescence of four compounds …
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Passive transport through biological membranes
… for biological systems. In Chapter 2 I use fluorescence methods to determine the passive transport of molecules with self intrinsic fluorescence through lipid membranes. We have built a unique fluorescence microscope which is capable of visualizing the fluorescence of molecules excited with …
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Surface Characteristics of Bacillus Spores
… scanning electron microscopy, and steady-state fluorescence by spectroscopy. The morphology of spores was similar within taxonomic groups and dissimilar between groups. Spore hydrophobicity ranged from 0.3% to 65% and all spores had fluorescence emission peaks at 335 nm and 450 nm. The …
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Defining the functional response of Mycobacterium sp. infected macrophages to structural variants of Interferon gamma (IFN-y)
… and analysed using circular dichroism (CD) and intrinsic fluorescence spectroscopy to probe secondary and tertiary structural organisation. This approach enabled direct comparison of structural behaviour between wild-type and variant proteins, as well as across different expression systems. CD …
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Fluorescence Detectors for Proteins and Toxic Heavy Metals
… technique was based on two-photon excitation intrinsic protein fluorescence using a visible 532 nm diode-pumped nano laser as the excitation source. Proteins that exhibit intrinsic fluorescence must contain at least one tryptophan, tyrosine, or phenylalanine residue in their amino acid …
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Multimodal spectroscopy : real-time diagnosis of breast cancer during core needle biopsy
… the potential of Raman, reflectance, and intrinsic fluorescence spectroscopy to provide biochemical and morphological information for discriminating breast lesions. Each modality was evaluated individually, as well as in combination, using a technique known as multimodal spectroscopy …
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Techniques and Applications of Mesoscopic Fluorescence Imaging
… millimeter penetration depth. By introducing fluorescence dyes or with intrinsic fluorescence, much higher contrast of images can be recorded compared to reflection imaging. To assess the characteristics of fluorescence imaging system, in the first part of this thesis I will discuss 3D …
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Studying the Structural Dynamics and Aptamer-Ligand Interactions in the Cocaine-Binding Aptamer through Fluorescence Spectroscopy
… results discussed in this study demonstrate how intrinsic fluorescence of ligands was exploited for aptamer-ligand binding and thermal stability analyses. Furthermore, photoisomerization of stilbene coupled with ligand-induced binding mechanism of the cocaine-binding aptamer were employed for the …
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The Development of 2,3-Diarylindenes as Integrated Fluorescent Estrogens
… to geometric considerations and competition from fluorescence and intersystem crossing. Crystallography indicated more planar topography for the indenes and indenones as compared to other triarylethylenes. The estrogen receptor binding affinities of the indenes, indenones, and other …
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Drinking Water Quality Monitoring
… microbial contamination in drinking water using intrinsic fluorescence of the microorganisms. The prototype, “Blinky”, uses LEDs that emit light at 365nm, 590nm, and 635nm for ultraviolet, amber, and red light, respectively. At 365 nm, the cellular components excited include reduced pyridine …
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Targeting protein: protein interaction sites for drug development against voltage-gated sodium channels
… assays, patch clamp electrophysiology, and intrinsic fluorescence studies, we have identified the β9 loop at the interface of FGF14 as critical for binding to Nav1.6 channels. Based on this information, we have identified a short sequence on FGF14 and designed a peptidomimetic (ZL181) …
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MOLECULAR CHARACTERIZATION OF THE PUTATIVE NUCLEOTIDE- BINDING AND HYDROLYZING ACTIVITIES OF THE ELLIS VAN CREVELD (EVC) PROTEIN
… of GTP analog caused the quenching of the intrinsic fluorescence of the protein, suggesting interaction between the two molecules. The fluorescence gradually recovered as the nucleotide or its hydrolysis product dissociated from the protein.</p> <p><br /> GTP hydrolysis assays showed that …
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MarR Family Transcriptional Regulators from Streptomyces coelicolor
… by the ligand urate, which also quenches the intrinsic fluorescence of PecS, indicating a direct interaction between urate and PecS. In vivo measurement of gene expression showed that activity of pecS and pecM genes is significantly elevated after exposure of S. coelicolor cultures to urate. …
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Structural basis for the function of the transcriptional regulator KstR from Mycobacterium tuberculosis
… shift assay, surface plasmon resonance and intrinsic fluorescence quenching assay). Protein-DNA binding assays have demonstrated that the 21-amino-acid extension at the N-terminus of KstR imposes a positive but non-essential role for KstR-DNA interaction. Protein-ligand binding assays and …
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A Characterization of the Effect of Apolipoprotein AII on the Structure and Function of Apolipoprotein AI in Reconstituted High Density Lipoproteins
… binding of apoAII-DNS to AIrHDL was monitored by fluorescence polarization, which showed that apoAII bound rapidly and irreversibly. The intrinsic fluorescence of apoAI enabled the detection and quantitation of displaced apoAI by the change in $\lambda$max of Trp residues in the presence of 1.5 M …
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Investigating the Modification of Spontaneous Emission using Layer-by-Layer Self-Assembly
… nanostructures. We studied the modification of fluorescence dynamics using a polymer spacer layer fabricated through layer-by-layer (LbL) self-assembly. The advantages of this method are numerous: The self-assembled spacers can possess exceptional smooth surface morphology; The thickness of the …
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Purification and characterization of A[beta] and probing the interactions between myricetin and A[beta] using various spectroscopic methods
… complex formation was visualized by myricetin's intrinsic fluorescence. ThT fluorescence assay and CD study showed that myricetin can inhibit A[beta](25-40) and A[beta](1-42) from forming [beta]-sheet structured fibril. CD and UVRR studies further evidenced conformational changes from both …
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Mechanisms and measurements of sialidase in pneumonia
… or product via a spectroscopic method (e.g. fluorescence) to monitor the rate of the reaction. Neither 3'-sialyllactose,6'-sialyllactose nor their products display intrinsic fluorescence and only have absorbance. Unfortunately, the signals are often weak and broad and so it is not a sensible …
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Investigating the self-assembly and molecular chaperone ability of casein proteins and polyproline
Caseins are a group of intrinsically disordered proteins (IDPs) present in the milk of all mammals. A property common to many IDPs is their propensity to assemble into amyloid fibrils, which are associated with many age-related diseases. Based on the stability of their predicted 'steric zipper' …
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Investigating the self-assembly and molecular chaperone ability of casein proteins and polyproline
Caseins are a group of intrinsically disordered proteins (IDPs) present in the milk of all mammals. A property common to many IDPs is their propensity to assemble into amyloid fibrils, which are associated with many age-related diseases. Based on the stability of their predicted 'steric zipper' …
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