Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"intrinsic disorder"”.
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Intrinsic Disorder in Transcription Factors
… evidence suggested that high abundance of intrinsic disorder in eukaryotic genomes in comparison to bacteria and archaea may reflect the greater need for disorder-associated signaling and transcriptional regulation in nucleated cells. The major advantage of intrinsically disordered proteins …
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A study of intrinsic disorder and its role in functional proteomics
… of an ensemble of flexible regions popularly as `intrinsically disordered' or `unstructured'. Several proteomic studies have corroborated the existence of either wholly disordered proteins or proteins that contain regions of disorder in them. The purpose of this dissertation was to investigate the …
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Organised Chaos: defining different degrees of intrinsic disorder by molecular dynamics methods
Intrinsically disordered proteins (IDPs) and regions (IDRs) are defined as proteins, or proteins’ regions, that lack a stable 3D structure. The nature of the IDP(R)s amino acid residues and their sequence prevents these systems from folding. IDPs and IDRs are ubiquitous in biology, playing many …
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Intrinsic Disorder and Protein Evolution: Amino Acid Composition of Proteins in Last Universal Ancestor
… et al. (1997) amino acids G, D, E, P and S are disorder-promoting residues and C, F, W and Y are order-promoting residues this means that the early or the ancient amino acids were disorder promoting and the order promoting residues came late into the genetic code. These observations led to the …
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Covalent modification and intrinsic disorder in the stability of the proneural protein Neurogenin 2
… post-translational modifications and structural disorder. Firstly I will describe work focused on ubiquitylation of xNgn2, targeting it for proteasomal degradation. xNgn2 is ubiquitylated on lysines, the recognized site of modification. I will discuss the role of lysines in ubiquitylation and …
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Structural Characterization of the C-terminus of the Cochlear Motor Protein Prestin: Intrinsic Disorder Linked to Voltage Sensor Function
<p> The sensitivity of mammalian hearing relies upon the ability to amplify sound. Outer hair cells (OHCs) in the cochlea have motile properties that support this function. The motor protein called prestin resides in the lateral wall of the OHCs. Prestin undergoes voltage-dependent conformational …
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Intrinsically disordered proteins in molecular recognition and structural proteomics
Intrinsically disordered proteins (IDPs) are abundant in nature, being more prevalent in the proteomes of eukaryotes than those of bacteria or archaea. As introduced in Chapter I, these proteins, or portions of these proteins, lack stable equilibrium structures and instead have dynamic …
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Evolution of protein structure, function, and dynamics in biological systems
… level cellular and viral proteomes leverage disorder to achieve advanced functionality and functional economy, respectively. Intrinsic disorder analysis at the protein domain level revealed that early protein domains were ordered and therefore, disorder is a benefit that was acquired later in …
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Effect of disorder on the vortex dynamics in superconducting Josephson junction arrays and clusters
We have studied the effect of intrinsic disorder on the transport properties of large Josephson junction arrays and clusters. In large arrays, intrinsic random disorder results in the washing out of features in the magnetoresistance. It also gives rise to multiple onsets in the resistive transition …
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Dynamics characterization for designing functional soft materials
… recent decades by recognition that features like intrinsic disorder, conformational distribution, and solvent dynamics often play a part which is equally fundamental to the binding and reactivity of these materials. The same revelation holds for many other functional soft materials, including …
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Cloning, Expression and Purification of the Different Human Haptoglobin Chains and Initial Characterization by Mass Spectrometry
… included work done to elucidate the regions of disorder in the protein DnaT. DnaT is one of the primosomal proteins that is an essential component of the PriA replication restart pathway. Very little is known about this protein, its structure, and its actual mode of function. Several attempts to …
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An investigation of intrinsically disordered proteins in Gram-negative bacteria
… these discoveries in the study of proteins with intrinsic disorder are explored, directions for future studies are discussed, and remaining questions are identified.
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Biochemical Characterization of the DUF3328 Protein in the Biosynthesis of Cyclic Peptide Cyclochlorotine
… posed by their membrane association and intrinsic disorder. In vitro assays demonstrated that CctR is a copper-dependent enzyme that hydroxylates cyclochlorotine, and dimerization is essential for the activity. Mechanistic studies using isotopic labeling confirmed dioxygen as the oxygen …
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Exploring induced secondary structure and unmethylated DNA binding domains of methyl CpG binding protein 2 (MeCP2)
… evidence that proves MeCP2 is an archetypical intrinsically disordered protein acting as a global ATP independent chromatin architectural protein. The ubiquity of MeCP2 in vertebrate neuronal nuclei has only recently been realized and has focused my investigations. Results from my work …
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NMR studies of quantum thermalization
… quantum thermalization phenomena. Leveraging the intrinsic disorder, a novel method to detect spin dynamics at single-site level is introduced. The method is applied to study hydrodynamics emerged from thermalizing quantum systems. In an interacting integrable system, coexistence of ballistic …
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Chemical Biology Tools for Biochemical and Functional Analysis of Myosin Phosphatase
… To overcome challenges posed by PPP1R12C’s intrinsic disorder and the dynamic assembly of the complex, we employed mammalian expression systems and AlphaFold modeling to predict structure and optimize expression. A ligand-inducible degron system was developed by fusing PPP1R12C to a …
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Evolutionary, structural and functional features of cellular signalling networks
… as experimental methods used to detect them. Intrinsic disorder in proteins is introduced as a structural feature which may influence local evolutionary rates. Several examples of complex PTM signalling systems are then described. Chapter 2 presents a study of the evolution of modified amino …
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Metabolism of Alpha-Synuclein by the 20S Proteasome
… is one of several common neurodegenerative disorders that are related by the intracellular aggregation of the neuronal protein alpha-synuclein (alphaSyn) that normally associates with synaptic vesicles. Within the aggregates, a fraction of alphaSyn is truncated at the C-termini, and these …
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Intrinsically Disordered Proteins within the Genome
… structure are particularly enriched in `Intrinsic Disorder’ – a class of proteins lacking a well-defined structure but existing as a dynamic ensemble of rapidly interchanging states. While folded proteins with well-defined structures are amenable to be characterized through standard …
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