Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 28 for “"intravital microscopy"”.
-
Improving the delivery and efficacy of molecular medicine via extracellular matrix modulation : insights from intravital microscopy
… recovery after photobleaching combined with intravital microscopy to quantitate the transport properties of the tumor interstitium. We found that the presence of fibrillar collagen correlated with hindered diffusion in vivo, and also in vitro, in collagen gels prepared to mimic tumor …
-
Role of neurogenic inflammation in the pathogenesis of acute pancreatitis and associated lung injury
… pancreatic microcirculation was visualized using intravital microscopy. CP96,345 significantly decreases the number of rolling and adherent leukocytes. In summary, blockade of SP-NK1R system, the major component of NI, has been proven to be effective in the treatment of AP and associated lung …
-
Ischemia Impairs Vasodilation in Skeletal Muscle Resistance Artery
… gracilis muscle contraction 14 days later using intravital microscopy. Functional vasodilation was significantly impaired in ischemic mice (14.4±4.6% vs. 137.8±14.3%, p<0.0001 n=8) and non-ischemic adult mice (103.0±9.4% vs. 137.8±14.3%, p=0.05 n=10). In order to analyze the cellular mechanisms …
-
The role of platelets and their associated recruitment mechanisms, in intestinal ischaemia reperfusion injury
… GPVI, GPIb-IX-V and the integrins IIb3 and 21. Intravital microscopy was utilised to monitor individual platelet adhesion and microthrombus formation in anaesthetised mice undergoing intestinal IR injury \(in\) \(vivo\) using a novel dual labelling methodology. This study focussed on the …
-
The Impact of Outward Remodeling on Vasodilation in Skeletal Muscle Resistance Arteries
… contraction 7 and 28 days post-surgery using intravital microscopy. Enlarged resting diameter, consistent with collateral enlargement, and impaired vasodilation was observed at day 7, but not at day 28. To determine if impaired functional vasodilation is due to impaired endothelial- or smooth …
-
Investigating Pulmonary Immune Responses that Detect and Counteract Gram-negative Bacterial Infections
… interactions that resulted in immunothrombosis. Intravital microscopy and novel single cell behavioral analysis revealed that cathelicidin initiated neutrophil swarming and clustering, which preceded LTB4 dependent immunothrombosis. These immunothrombi trapped bacteria and promoted platelet …
-
Late Effects of Ionizing Radiation on Normal Microvascular Networks
… reduce heterogeneity due to spatial variation. Intravital microscopy was used to measure both structural and functional parameters. <br />Geographic Information Systems (GIS) technology was used to establish network topology. At all late time points, the diameter of irradiated vessels was …
-
Visualizing Dendritic Cells In Vivo
… in tolerance and immunity; this was achieved by intravital microscopy of fluorescently-labeled DCs and lymphocytes in the inguinal lymph nodes. We generated mice that specifically expressed EYFP in their dendritic cells and observed their behavior in living LNs; we found that in the steady state, …
-
A zebrafish model to study the schistosome egg granuloma
… mansoni eggs into zebrafish, followed by intravital microscopy to observe the formation of the schistosome egg granuloma. Within the zebrafish, eggs induce the formation of epithelioid granulomas, as in mammalian models. I find that while mature schistosome eggs induced granuloma …
-
Seeing Inside a Concussion: The Role of Microglia in Maintaining the Blood-Brain Barrier in Repetitive Mild Traumatic Brain Injury
… order to study the neuroimmune response. Using intravital microscopy, we observe microglia to become progressively activated with repetitive injury. Activated microglia phagocytose dysfunctional astrocytes following repetitive concussion. Additionally, microglia rapidly seal areas of blood-brain …
-
Effekt einer Antagonisierung der alpha- v-Integrine auf Angiogenese, Wachstum und Metastasierung solider Tumoren in vivo
… endothelium interaction were analyzed using intravital microscopy. In an additional study the effects on growth and metastasis of subcutaneous A-Mel-3 were quantified. Results: Functional vessel density was markedly reduced on day 3 in treated animals compared to controls (37.2+/-12.1 vs. …
-
Integrative Multivariate Analysis of Mouse Liver Acini
… musculus’ (mice) liver was performed. To perform intravital microscopy (IVM), the methodology required the creation of a 3D printed platform called RHIT (Reusable Hepatic Imaging Tool) that enabled seamless integration into IVM protocols and stabilized the tissue for dynamic and acute imaging for …
-
Erythrocyte-Associated Transients in Capillary PO2 in the Rat Spinotrapezius Muscle During Hemodilution with Hespan and a Hemoglobin-Based Oxygen Carrier
… sixteen Sprague-Dawley rats was exteriorized for intravital microscopy measurements in capillaries. Capillary PO2 was measured using Pd-porphyrin phosphorescence quenching microcopy. The hemodiluents used in isovolemic hemodilution included Hespan, a non-oxygen carrying plasma expander, and …
-
Effect of Nitric Oxide on Oxygen Consumption of Skeletal Muscle
… use it as indicator of local metabolic changes. Intravital microscopy and phosphorescence quenching were used to record PISFO2 in resting muscle for 120 s before, 60 s during, and 420 s after a period of tissue compression that abruptly halted perfusion. Control VO2 measurements were made, …
-
Beta Cell Heterogeneity in the Acute Interferon Alpha Response
… β-cell function but detrimental in excess. Using intravital microscopy and a β-cell-specific ROS biosensor, we identified a subset of β-cells that rapidly produce ROS in response to IFN-α. Interestingly, phenotyping data from the donors indicated that healthier β-cells were more likely to exhibit …
-
The Impacts of Arterial Occlusion, Sex, and Exercise on Arteriogenesis and Functional Vasodilation
… rest and after gracilis muscle contraction using intravital microscopy. No major sex differences were observed in any group. At day 7, both the resting and dilated diameters were increased, while vascular reactivity was minimal. By day 28, resting diameter decreased while maximal diameter was …
-
Novel Imaging-Based Techniques Reveal A Role For Pd-1/Pd-L1 In Tumor Immune Surveillance In The Lung
… within the tumor microenvironment, we employed intravital microscopy to investigate the in vivo impact of PD-L1 blocking antibody upon tumor-associated immune cell migration. However, current analytical methods of intravital dynamic microscopy data lack the ability to identify cellular targets …
-
Targeting Breast Cancer With Bacteriophage Associated Silicon Particles
… in <em>in vivo</em> conditions evidenced by intravital microscopy studies that showed up to 4 fold increase in the number of CRKL targeted BASP accumulating in the tumor vasculature compared to both S1MP and fd-tet non-targeted BASP. <em>In vivo</em> experiments also showed that BASP maintain …
-
Shortwave infrared imaging and its translation to clinically-relevant designs
… for in vivo SWIR fluorescence imaging, including intravital microscopy, noninvasive, real-time imaging in blood and lymph vessels, and tumor-targeted imaging with IRDye 800CW, a dye being tested in clinical trials. Thus, we suggest that there is significant potential for SWIR imaging to be …
-
Migration of tumor cells and leukocytes in extracellular matrix : proteolytic and nonproteolytic strategies for overcoming tissue barriers
… combination with time-lapse video-, confocal- or intravital multiphoton-microscopy, and computer-assisted cell tracking. Expression of proteases, including several MMPs, ADAMs, serine proteases and cathepsins, was shown by flow cytometry, Western blot, zymography, and RT-PCR. Protease activity by …
Page 1 of 2