Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 61 for “"intravital"”.
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Intravital diagnostics of neurodegenerative diseases
Prionopatie, taktiež nazývané transmisívne spongiformné encefalopatie (TSE), a synukleínopatie predstavujú skupinu neurodegeneratívnych ochorení, ktoré sú asociované s akumuláciou nesprávne zložených proteínov (prión a α-synukleín) prevažne v centrálnom nervovom systéme. Kvôli absencii spoľahlivého …
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Intravital imaging of metastasis in zebrafish
… These events are dynamic and so require intravital imaging to investigate. However, the intravital imaging of these events in mice is challenging. Sites of metastasis are often in vital organs that are inaccessible to microscopy without surgical intervention. Furthermore, circulating …
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Intravital Imaging of Dynamic Behaviors of Leukocytes in UVB-induced Skin Inflammation
… responding to 3000J/m2 UVB in the skin. Using intravital confocal microscopy and genetically manipulated transgenic mice, we have found that neutrophils are recruited to inflammatory sites immediately after UVB irradiation. Skin-infiltrating neutrophils can interact with LCs upon activation and …
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Visualizing The Dynamics of Immune Surveillance In Brain Tumors By Intravital Multiphoton Microscopy
… of immune surveillance in brain tumors by intravital multiphoton microscopy</strong></p> <p>By</p> <p>Felix I. Nwajei, MD</p> <p>Supervisory Professor: Tomasz Zal, Ph.D.</p> <p>Brain tumors (BTs) generally have a bad prognosis despite conventional treatment strategies. Immunotherapy is a …
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Improving the delivery and efficacy of molecular medicine via extracellular matrix modulation : insights from intravital microscopy
… recovery after photobleaching combined with intravital microscopy to quantitate the transport properties of the tumor interstitium. We found that the presence of fibrillar collagen correlated with hindered diffusion in vivo, and also in vitro, in collagen gels prepared to mimic tumor …
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High performance lattice boltzmann method yield-stress calculations based on intravital images of clot formation in live mice
Thrombo-embolic infarction is the major cause of mortality and morbidity in the United States, causing over 1 million strokes, heart attacks and other life-threatening thrombotic events each year in the United States. Conversely, deficiencies in these processes result in severe bleeding risks. The …
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Multimodal intravital imaging of tissue structure and cell dynamics in skin using integrated optical coherence and multiphoton microscopy
This dissertation describes the development and application of multimodal optical imaging methods for providing enhanced visualization of living skin. In vivo imaging of skin is of great interest for research applications to study fundamental aspects of skin biology as well as for clinical …
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Unraveling the Effects of Nicotine-Derived Nitrosamine Ketone (NNK) on Healthy and Neurodegenerative Brain via Intravital Imaging and Multiscale Ex Vivo Imaging Approaches.
… the use of a transgenic mouse (CX3CR-1GFP) with intravital neuroimaging. We show that NNK given intranasally over acute (4-7days) and chronic (12 weeks) exposures, leads to disruption of the BBB and heterogenous microglia activation. Machine learning algorithm was employed to segment microglial …
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Identification of intravasation sites for metastatic biomarker development
… coupled with fluorescent streptavidin for intravital imaging. Combining these components, we were successful in performing intravital imaging to identify differentially labelled blood vessels, indicating possible intravasation sites. Future work will involve confirmation of the specificity …
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Novel Imaging-Based Techniques Reveal A Role For Pd-1/Pd-L1 In Tumor Immune Surveillance In The Lung
… within the tumor microenvironment, we employed intravital microscopy to investigate the in vivo impact of PD-L1 blocking antibody upon tumor-associated immune cell migration. However, current analytical methods of intravital dynamic microscopy data lack the ability to identify cellular targets …
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Mechanisms of Edema Clearance: The Central Role of Lymphatic Vessels and LYVE1 in Fluid Homeostasis
… edema. We utilized its optical transparency and intravital imaging capabilities to characterize the dynamic lymphatic responses during edema formation and resolution. Using this platform, we identified key structural and molecular changes that accompany compensatory lymphangiogenesis. …
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Role of neurogenic inflammation in the pathogenesis of acute pancreatitis and associated lung injury
… pancreatic microcirculation was visualized using intravital microscopy. CP96,345 significantly decreases the number of rolling and adherent leukocytes. In summary, blockade of SP-NK1R system, the major component of NI, has been proven to be effective in the treatment of AP and associated lung …
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Migration of tumor cells and leukocytes in extracellular matrix : proteolytic and nonproteolytic strategies for overcoming tissue barriers
… combination with time-lapse video-, confocal- or intravital multiphoton-microscopy, and computer-assisted cell tracking. Expression of proteases, including several MMPs, ADAMs, serine proteases and cathepsins, was shown by flow cytometry, Western blot, zymography, and RT-PCR. Protease activity by …
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Ischemia Impairs Vasodilation in Skeletal Muscle Resistance Artery
… gracilis muscle contraction 14 days later using intravital microscopy. Functional vasodilation was significantly impaired in ischemic mice (14.4±4.6% vs. 137.8±14.3%, p<0.0001 n=8) and non-ischemic adult mice (103.0±9.4% vs. 137.8±14.3%, p=0.05 n=10). In order to analyze the cellular mechanisms …
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Imaging Biomarkers of Response to Immune Checkpoint Inhibition in Melanoma
… emission computed tomography (SPECT) imaging and intravital imaging are explored as part of a co-clinical study on metastatic melanoma patients and syngeneic mouse models of cancer immunotherapy.
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The role of platelets and their associated recruitment mechanisms, in intestinal ischaemia reperfusion injury
… GPVI, GPIb-IX-V and the integrins IIb3 and 21. Intravital microscopy was utilised to monitor individual platelet adhesion and microthrombus formation in anaesthetised mice undergoing intestinal IR injury \(in\) \(vivo\) using a novel dual labelling methodology. This study focussed on the …
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Tumor vasculature and microenvironment during progression and treatment : insights from optical microscopy
… and therapy. By modifying the technique of intravital multiphoton microscopy (MPM) to perform phosphorescence quenching microscopy, I developed a non-invasive method to quantify oxygen tension (p02) in living tissue at high three-dimensional resolution. To probe functional changes in the …
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Macrophage-mediated resistance mechanisms against MAPK inhibitory by cancer therapeutics
… signaling networks, we develop an intravital imaging strategy to monitor the influence of tumor-associated macrophages on cancer cell kinase activity dynamics. Finally, we rationally design a nanotherapy to exploit inhibitor-induced immunomodulation and crosstalk. Overall, we …
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Cellular and molecular mechanisms of liver regeneration
… parallel, a novel experimental system to permit intravital multiphoton microscopy of the regenerating liver following partial hepatectomy in mice was developed and validated. Intravital imaging of mouse liver was performed with a range of cellular labels, combined with a fluorescent cell cycle …
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The Impact of Acetylcholine Receptors on Osteocytes and Bone Mechanoadaptation
… rates to anabolic loading. We also used intravital imaging via two-photon fluorescent microscopy to visualize the Ca2+ dynamics in osteocytes during active mechanical loading of the bone in living mice. Finally, we explore the ability of light-sheet fluorescent microscopy to create high …
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