Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"intratumor heterogeneity"”.
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The impact of intratumor heterogeneity on anti-tumor immunity
… impacts the anti-tumor immune response. High intratumor heterogeneity (ITH), where the majority of neoantigens is expressed subclonally, is correlated with poor clinical response to CBT and weaker anti-tumor immunity. The mechanism by which ITH blunts tumor-reactive CD8+ T cells is still …
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Integrating Bulk and Single-Cell Transcriptomics to Investigate Intratumor Heterogeneity
… for both technical artifacts and biological heterogeneity.</p>
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Statistical Methods For Resolving Intratumor Heterogeneity With Single-Cell Dna Sequencing
… and treatment resistance. Such genetic intratumor heterogeneity plays a role in the process of clonal evolution that underlies tumor progression and treatment resistance. Single-cell DNA sequencing is a promising experimental method for studying intratumor heterogeneity, but brings …
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Impact of Intratumor Heterogeneity and The Tumor Microenvironment In Shaping Tumor Evolution and Response to Therapy
<p>Intratumor heterogeneity (ITH) is a crucial challenge in cancer treatment. The genotypic and phenotypic heterogeneity underlying diverse cancer types leads to subclonal variation, which may result in mixed or failed response to therapy. The heterogeneity at the tumor level, along with the tumor …
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Genetic Profiling in Soft Tissue Sarcoma
… account for ~1% of all malignancies. Frequent heterogeneity and pleomorphism along with suboptimal diagnostic reproducibility and insufficient prognostic markers make clinical management of these tumors difficult. This thesis has applied microarray-based gene expression and copy-number …
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Investigating Metastatic Lineage In Colorectal Cancer By Single Cell Dna Sequencing
… difficult to study, in part due to the extensive intratumor heterogeneity at both the primary and metastatic tumor sites, and the low frequency of subclones with metastatic potential. Previous studies have applied conventional bulk next-generation sequencing (NGS) methods, which have limited …
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Genomic Evolution of Chemoresistance In Triple-Negative Breast Cancer Delineated By Single Cell Sequencing
… is an aggressive subtype that displays extensive intratumor heterogeneity and frequently (46%) develops resistance to neoadjuvant chemotherapy (NAC). Currently, the genomic basis of chemoresistance remains poorly understood. An important question is whether resistance to chemotherapy is driven by …
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TRANSLATIONAL RESEARCH TARGETING BILIARY TRACT CANCER
… Based on these mice models, intratumor heterogeneity and diverse drug responses will be explored. To improve the ratio of well differentiated CCA, we will use bile duct ligation technique instead of HTVI to make sure that plasmids can enter biliary tract directly.</p> <p>In …
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Simultaneous SNV calling and Phylogenetic Inference for Single-cell Sequencing Data
… provides a powerful approach for elucidating intratumor heterogeneity by resolving cell-to-cell variability. However, it also poses additional challenges including elevated error rates, allelic dropout, and non-uniform coverage. Variant calling in this context is the task of identifying …
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Evaluation and Optimization of the Translational Potential of Array-Based Molecular Diagnostics
… is focused on our evaluation of the impact of intratumor heterogeneity on precision in microarray-based assays in breast cancer. We conducted genome-wide expression profiling on 50 needle core biopsies from 18 breast cancer patients. Global profiles of expression were characterized using …
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Combinatorial Treatments and Technologies for Safe and Effective Targeting of Malignant Gliomas Using High-Frequency Irreversible Electroporation
… tissues beyond the visible tumor margin, intratumor heterogeneity, hypoxic core, and the presence of the blood-brain barrier (BBB) that restricts the penetration of large therapeutic agents, all pose formidable challenges for effective therapeutic intervention. The standard of care for GBM …
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Advancements in the Treatment of Malignant Gliomas and Other Intracranial Disorders With Electroporation-Based Therapies
… the solid tumor margin; and gross, macroscopic intratumor heterogeneity. These characteristics complicate treatment of GBM with standard of care, resulting in abysmal prognosis. Electroporation-based therapies have emerged as attractive alternates to standard of care, demonstrating favorable …
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Bioengineering of Heterogenous Glioblastoma Multiforme Microenvironment
… microenvironment (GBME) exhibits high heterogeneity, vast cell-to-cell interactions, and stiff mechanical properties. To produce in vitro models mimicking the GBME features, GBM organoid (GBO) models have been developed. Conventional organoid studies rely on growing them in serum-free …
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Computational imaging and multiomic biomarkers for precision medicine: characterizing heterogeneity in lung cancer.
… cases. The inherent intra-tumor and inter-tumor heterogeneity in lung tumors has been linked with adverse clinical outcomes. A well-rounded characterization of tumor heterogeneity by personalized biomarkers is needed to develop precision medicine treatment strategies for cancer. Large-scale …
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Reconstructing Mutational Lineages In Breast Cancer By Multi-Patient-Targeted Single Cell Dna Sequencing
<p>Triple negative breast cancer (TNBC) is an aggressive subtype of breast cancer with high rates of metastasis and recurrence, where TNBC patients have a poor 5-year survival and ~50% are non-responsive to chemotherapy. Aneuploidy is a cancer hallmark that is pervasive in over 90% of breast cancer …