Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 61 for “"intracellular transport"”.
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Characterizing and Influencing Intracellular Transport
… such as facilitating ion gradients and intracellular transport, the movement of cargo through a viscous, crowded cytosol. This latter point is especially important because many diseases, including Alzheimer’s disease, are associated with aberrant transport. Thus, determining how a cell …
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Force studies of intracellular transport
… like kinesin-1 and dynein are necessary for intracellular transport and a variety of other functions in the cell. They have been well characterized in simplified, single-motor, in vitro systems, but less is known about their mechanical properties in vivo, in a more complex, multi-motor …
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Small Molecules as Probes for Cell Division and Intracellular Transport
… powerful probes of cell division mechanisms and intracellular transport processes. Phenotype-based screens with chemical libraries have been used to identify such inhibitors. However, probes for most proteins are still not available, and the requirements on compound collections to yield such …
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Characterization and cooperative dynamics of kinesin motor proteins in intracellular transport
Intracellular transport is typically carried out by teams of motor proteins rather than by isolated molecules, yet the rules governing multi-motor coordination remain poorly understood because motor number, spacing, and composition are difficult to control experimentally. In this dissertation, I …
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Intracellular Transport in Cancer Treatments: Carbon Nanohorns Conjugated to Quantum Dots and Chemotherapeutic Agents
… often limited by bulk and cellular barriers to transport. Nanoparticle or chemotherapeutic compound intracellular transport has implications in understanding therapeutic effect and toxicity. The scope of this thesis was to study the intracellular transport of carbon nanohorns and to improve the …
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The folding, assembly, and intracellular transport of murine class I major histocompatibility complex heavy chains
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1997.
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Studies on the internalization and intracellular transport of horseradish peroxidase in Chinese hamster ovary cells
… endocytic vesicles. A minimum of four steps in transport have been identified based on their sensitivity to inhibitors. HRP transport, identified by Percoll density gradient fractionation, was inhibited at 17°C and was sensitive to pH elevators (NH₄Cl, monensin, HEPES) and ATP depletion …
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HDAC6 activity is required for efficient polarization and intracellular transport of organelles in directionally migrating cells
… to facilitate directed organelle and vesicle transport. This ultimately establishes a persistent leading edge and represses random migration. Given that tubulin is a known substrate of HDAC6 and since microtubules (MTs) are known to play an important role in polarization of directionally …
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Poxvirus A51R Proteins Directly Bind to Microtubules to Antagonize Microtubule Dynamics, Intracellular Transport, and a Cell-intrinsic Antiviral Response
… utilizes kinesin motor proteins on MTs to transport newly formed virions, yet how VV regulates this virion transport was previously unknown. Here, I demonstrate that poxvirus A51R proteins directly bind to MTs, establishing them as bona fide MT-associated proteins (MAPs). Notably, VV A51R …
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Mutational and functional analyses of the dynein heavy chain indicate the existence of an apical-sistal transport cycle in cells
… and function of eukaryotic cells depend on intracellular transport governed by the microtubule based molecular motors cytoplasmic dynein and kinesin. Dynein carries out the inward transport of cargos whereas kinesins are responsible for the outward movement. Although intracellular transport …
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Second Harmonic Generation – A Novel Approach in Retinal Imaging
… structural support and serving as a railroad in intracellular transport. We demonstrate the application of SHG microscopy to the following studies: 1) Can changes in MT conformation be detected when treated with a stabilizing drug (Taxol); 2) if disruption in MT precedes loss of axons in a mouse …
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Biochemical and immunocytochemical analysis of three novel microtubule-associated proteins in Drosophila melanogaster and the rat brain
… cells. Some of their diverse functions include intracellular transport, cell division and maintenance of cell shape. Microtubules are composed of highly conserved proteins, the $\alpha$- and $\beta$-tubulins. Many of the functions associated with microtubules are thought to be due to the …
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Genetics and functions of the SARS coronavirus spike protein
… S-mediated cell-to-cell fusion, while intracellular transport and cell-surface expression were not adversely affected. Surprisingly, a carboxyl terminal truncation of 17 amino acids substantially increased S glycoprotein-mediated cell-to-cell fusion suggesting that the terminal 17 amino …
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A method for detecting nonequilibrium dynamics in active matter
… such as tissue folding, cell division and intracellular transport. It is important to determine the extent of such nonequilibrium activity during cellular processes to understand cell function. Here we present a framework for measuring nonequilibrium activity in biological active matter …
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The plant cytoskeleton as a transportation network
… the role of the cytoskeleton in long-distance intracellular transport. As it provides the basis for cell growth and biomass production, cytoskeletal transport in plant cells is of direct environmental and economical relevance. However, while knowledge about the molecular details of the …
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FUNCTIONAL CHARACTERIZATION OF DENND5A/RAB6IP1 IN EARLY EMBRYONIC DEVELOPMENT
… GTP-binding protein that involves in intra-Golgi transport. In a yeast two hybrid screen by Bruno Goud group in 1995, they used Rab6 as bait and found several interacting cDNA clones including Rab6 interacting protein 1 or Dennd5a. Several Dennd5a interaction partners have been discovered, Rab6, …
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Identification of binding protein with kinesin18B
Microtubules play an important role in intracellular transport of vesicles and organelles in higher eukaryotic cells. This transport depends on the activity of motor proteins that interact with microtubules. Most kinesins move cargoes outwards toward the cytoplasmic membrane. Members of the kinesin …
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Ehrlichia chaffeensis TRP47 enters the nucleus via a MYND-binding domain-dependent mechanism and predominantly binds enhancers of host genes associated with signal transduction, cytoskeletal organization, and immune response
Ehrlichia chaffeensis is an obligately intracellular bacterium that establishes infection in mononuclear phagocytes through largely undefined reprogramming strategies including modulation of host gene transcription. In this study, we demonstrate that the E. chaffeensis effector TRP47 enters the …
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