Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"intracellular replication"”.
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Characterizing induced gene expression in Shigella flexneri following bile salt exposure
… epithelial cells to compare invasion ability and intracellular replication. The Congo red (CR) secretion assay was also used as a measure of virulence protein secretion from the type-III secretion system (T3SS), while interleukin-8 (IL-8) secretion from infected HT-29 cells was measured as a …
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Deciphering the molecular basis of the global emergence of non-tuberculous Mycobacteria as important human respiratory pathogens
… family Mycobacteriaceae. They are facultative intracellular bacilli causing opportunistic infections in individuals with pre-existing conditions such as respiratory and immune disorders. There has been a steady increase in the number of NTM infection reports in the past four decades and many of …
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Virulence associated functions and vaccine potential of the burkholderia mallei type three secretion effector BopA
… immunological and cellular responses. The intracellular survival of B. mallei within murine macrophage J774.2 cells requires the type III secretion system (TTSS), although the effector protein(s) involved are unknown. Additionally, previous reports have documented that B. mallei TTSS is …
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Trypanosoma Cruzi Phosphoinositide -Specific Phospholipase C (tcpi-Plc) and Its Role in Differentiation
… amastigotes, which is an essential step for the intracellular replication of these parasites.
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Illuminating Organellar and Molecular Organization of the Endomembrane System
… to form a Legionella-containing vacuole for intracellular replication. I reveal that MavQ generates phosphatidylinositol 3-phosphate on the endoplasmic reticulum membrane and that MavQ, together with the phosphatidylinositol 3-phosphatase SidP, self-organizes and propagates on the endoplasmic …
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Characterisation of the Virulence and Determinants of Virulence of <i>C. Neoformans</i> var.<i>grubii</i> Genotypes from HIV Infected and Uninfected Patients in Vietnam
… with more phenotypic variability, increased intracellular replication inside macrophages, and induction of more robust immune responses but apparent decreased virulence in mice. RNAseq profiling under log-phase growth highlights genotype-specific differential expression of key processes which …
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The development of novel HIV-1 vaccines using modified recombinant BCG
… synthesise pantothenic acid and thus has limited intracellular replication leading to less pathology. The UCT group has previously reported that HIV vaccines vectored by the Pasteur strain of rBCG ΔpanCD induced less pathology but improved immunogenicity as compared to Pasteur WT in the murine …
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The Role of Urease in the Pathogenesis of Edwardsiella ictaluri
… the ureG mutant strain was attenuated for intracellular replication while the WT strain showed more than a ten-fold increase in numbers of viable organisms recovered at 12 hours post infection (PI), even though there were no significant differences in initial uptake of either strain. Light …
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Identification of host factors required for cytosol entry by Shigella flexneri: Results of a genome-wide CRISPR/Cas9 screen
… further panel of microscopy, flow cytometry and intracellular replication assays. I showed that the multifunctional protein KHDRBS1, known to act as a signalling adaptor and regulator of inflammatory responses, facilitates cytosol entry and replication by *Shigella*. I showed that the …
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DISSECTION OF HOST-VIRUS INTERACTIONS IN THE HUMAN CENTRAL NERVOUS SYSTEM
… knockdown experiments of ARL13b show reduced intracellular replication in fibroblasts, identifying a potential proviral role for cilia-related proteins during herpesvirus infection. To conclude, through our genome-wide screens and transcriptional profiling we identified candidate neuronal …
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Intracellular parasitism of macrophages by Cryptococcus
… cells (especially macrophages), thus acting as intracellular parasites. This intracellular phase is thought to underlie the ability of the pathogens to remain latent for long periods of time within infected individuals. Here, we demonstrate that cryptococci can also manipulate host macrophages …
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Discovery and functional analyses of new components of the apical complex and the pellicle of Toxoplasma gondii
… in parasite gliding motility, invasion and intracellular replication. A possible explanation for these defects is that the annuli might participate in the regulation of secretion processes as constitutive microneme exocytosis was impaired after AAP6 knockdown. Furthermore, I found that AAP6 …
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Analysing M. abscessus-macrophage interactions and investigating treatments for M. abscessus and S. aureus intracellular bacterial infections, using a continuously growing, primary cell model of lung alveolar macrophages
… infection. The ability of MAB and SA to reside intracellularly helps them to avoid elimination by the host immune system, and confers a barrier to effective antimicrobial treatment. Therefore, the development of novel antimicrobial treatments, which show efficacy towards inhibition of bacteria …
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Virulence and pathogenic mechanisms of <i>Legionella pneumophila</i>
… <i>L.pneumophila</i>(CV) was isolated from its intracellular <i>in vivo</i> environment and these bacteria compared with the same strain grown on BCYE. The <i>in vivo</i>-grown CV showed no change in uptake by, or intracellular replication within, alveolar macrophages but, when Western blotted …