Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"intracellular cytokine staining"”.
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The Influence of Immunization Route On The Adjuvant Effect of Alpha-Galactosylceramide
… the innate immune mediating NKT cells to produce cytokines and activate downstream immune cells, resulting in development of humoral and cell mediated immune responses to co-administered antigens. While a single intravenous immunization of α-GalCer strongly activates NKT cells, multiple doses by …
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Optimisation of a flow cytometry antibody panel to detect BCG-induced innate responses in infants
… response to BCG was characterised by whole blood intracellular cytokine staining of banked, previously stimulated immune cells from 25, 10-week-old, HIV-uninfected, infants. The cohort is a subset of participants of a randomised control trial conducted in Worcester, South Africa. A 13-colour flow …
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Down-Regulation of Natural Killer Cell Activation in Response to Influenza Virus in Older Adults
… significant amount of IFN-7 , a hallmark Thl cytokine, in response to influenza infection. How aging influences human NK cell IFN-7 production in response to influenza virus has not been well documented. In this study we employed human peripheral blood mononuclear cells (PBMC) and performed …
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Developing Methods to Study Pathogenesis and Immune Response to HSV-2 in the Guinea Pig Model
… the ability of the antibodies to detect intracellular IFN production for flow cytometry (FC) analysis. The purified antibody was conjugated to a fluorochrome and applied to IFN producing guinea pig (gp) spleen cells post stimulation with PMA. FC data analysis showed this …
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Developing Methods to Study Pathogenesis and Immune Response to HSV-2 in the Guinea Pig Model
… the ability of the antibodies to detect intracellular IFN production for flow cytometry (FC) analysis. The purified antibody was conjugated to a fluorochrome and applied to IFN producing guinea pig (gp) spleen cells post stimulation with PMA. FC data analysis showed this …
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Ebna1-Specific T Cell Responses During Persistent Rhesus Lcv infection and The Development of a Novel Therapeutic Prototype Vaccine for Ebv-Associated Diseases
… macaques. We assessed frequency, phenotype, and cytokine production profiles of rhesus (rh)EBNA1-specific T cells by intracellular cytokine staining (ICS) and polychromatic flow cytometry. We found that most naturally infected animals had CD4+ and/or CD8+ T cells against rhEBNA1 and rhBZLF1, an …
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Characterisation of T cell responses to proteins expressed during Human Cytomegalovirus latency
… of these cells secreting the immunosuppressive cytokines interleukin (IL)-10 and transforming growth factor beta ($\beta$). However, little is known about the host immune response to other key latency-associated viral proteins; US28, UL111A, and UL144. Using overlapping peptide pools designed to …
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Characterising the magnitude and breadth of T cell responses to SARS-CoV-2 vaccination
… spike protein were characterized using intracellular cytokine staining interferon-γ (IFN-γ) or interleukin-2 (IL-2) and flow cytometry. The magnitude of T cell responses to full spike was examined, as well as crude T cell breadth, using 7 peptide pools spanning the entire length of the …
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THE ROLE OF CD4+ T MEMORY SUBSETS IN HIV INFECTION: IMMUNE RESPONSE IN PEOPLE WITH HIV AND LATENCY ESTABLISHMENT
… as target of infection as well as reservoir, sustaining long-lived, self-renewing pools of latently infected cells despite antiretroviral therapy (ART). Thus, TSCM are central to both protective immunity and the persistence of HIV reservoir. In people with HIV (PWHIV), vaccine-induced immune …
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Charakterisierung zellulärer Immunantworten von mit SIV immunisierten und infizierten Makaken
Die HIV-Infektion und die daraus resultierende AIDS-Erkrankung sind eine globale Herausforderung für die Menschheit. Für präklinische Studien zur Entwicklung eines Impfstoffes gegen diese Virusinfektion und für entsprechende Pathogenesestudien repräsentiert die Infektion von Makaken mit dem …