Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 3180 for “"intracellular"”.
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Engineering intracellular antibody libraries
The goal of this research is to understand how three different parameters affect single chain variable fragment (scFv) binding capacity. The parameters that were varied include the number of variable complementarity determining regions (CDRs), the number amino acids used to diversify CDRs, and …
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Computing traction forces, intracellular prestress, and intracellular modulus distribution from fluorescence microscopy image stacks
… of the prestress distribution accounting for intracellular inhomogeneity.
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Characterizing and Influencing Intracellular Transport
… such as facilitating ion gradients and intracellular transport, the movement of cargo through a viscous, crowded cytosol. This latter point is especially important because many diseases, including Alzheimer’s disease, are associated with aberrant transport. Thus, determining how a cell …
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Force studies of intracellular transport
… like kinesin-1 and dynein are necessary for intracellular transport and a variety of other functions in the cell. They have been well characterized in simplified, single-motor, in vitro systems, but less is known about their mechanical properties in vivo, in a more complex, multi-motor …
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Signalling to intracellular Ca2+ channels
… to sustained (60 min) stimulation. However, intracellular concentrations of cAMP were greater after sustained stimulation. Inhibition of protein kinase A (PKA) or exchange proteins activated by cAMP (EPAC) had no effect on the Ca$^{2+}$ signals evoked by carbachol after acute or sustained …
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INTRACELLULAR TARGETS OF SPHINGOSINE-1-PHOSPHATE
… compelling evidence to suggest that S1P exerts intracellular functions independent of S1PRs. However no bona fide intracellular targets of S1P have been identified. In my dissertation, I have identified a novel intracellular binding protein for S1P. This finding has important implications for …
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Intracellular delivery of rabbit monoclonal antibody
… and understand the functions and structures of intracellular proteins, more powerful tools are under demand. Recently, an increasing number of rabbit monoclonal antibodies (RabMAbs) have been approved to able to recognize subtle distinctions between the changes of intracellular proteins status. …
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Intracellular trafficking of Leishmania major peptidases
… to the lysosome. Thus, in this project, the intracellular localisations of eight other L. major peptidases were analysed by fluorescence microscopy, after tagging the enzymes with green fluorescent protein (GFP). The candidate peptidases were chosen by bioinformatics analyses and predictions …
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Intracellular parasitism of macrophages by Cryptococcus
… cells (especially macrophages), thus acting as intracellular parasites. This intracellular phase is thought to underlie the ability of the pathogens to remain latent for long periods of time within infected individuals. Here, we demonstrate that cryptococci can also manipulate host macrophages …
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Intracellular signalling by arachidonic acid metabolites
In intrauterine tissues, pro-inflammatory cytokines and prostaglandins (PGs) have been identified as key mediators in the maintenance of pregnancy and parturition. The rise in PGD2 detected in the amniotic fluid before labour prompted the research presented in this thesis which describes the …
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Mechanisms of TRIM21-directed intracellular degradation
… cascades, TRIM21 acts as a danger sensor and intracellular effector of humoral immunity. In addition to antibody binding, TRIM21 is a ubiquitin E3 ligase, an enzyme which catalyzes the formation of polymers of the protein ubiquitin around target substrates. When viruses coated in …
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TRIM21 Mediated Antibody-Dependent Intracellular Immunity
Antibodies have been extensively studied since the 1890s, revealing their importance in protective immunity and allowing their development in numerous research and therapeutic applications. However, these studies have focused almost exclusively on the extracellular effector mechanisms of antibodies …
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A novel intracellular protein delivery system - Magnesium phosphate nanoparticles with cationic lipid coating for catalase intracellular delivery
… those caused by alternations in the functions of intracellular proteins. However, clinical use of protein by intracellular delivery has been hampered by the instability due to proteins' physicochemical properties, and some barriers in the delivery pathway. This study was to prepare and test a …
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DESIGNING BIOMIMETIC NANOPARTICLES TO TACKLE INTRACELLULAR PATHOGENS
… to evade therapeutic clearance by creating intracellular reservoirs within host macrophages. Conventional drug delivery systems often fall short due to their lack of precision, limited intracellular access, and rapid clearance by the immune system. To address these limitations, we developed …
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Examining Intracellular Phosphorylation Gradients During Cell Division
<p>The dynamic cellular reorganization needed for successful mitosis requires spatial regulatory cues. I examine this problem at two different levels. First, I analyze a phosphorylation gradient for substrates of the chromosomal passenger complex (CPC). CPC is a conserved regulator involved in key …
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Pressure-Induced Intracellular Signaling in Isolated Arteries
… mechanical stresses through the activation of intracellular signaling pathways which result in cell-specific responses such as hypertrophy, hyperplasia, proliferation, and migration. Vascular smooth muscle of the arteries have been shown to be sensitive to mechanical stimuli such as stretch, …
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Novel Fluorescent Sensors for Dynamic Intracellular Signalling
Protein kinase C (PKC), comprised of ten isozymes, is an abundant regulator of cellular function. PKC activity in living cells is commonly assayed in in vitro experiments with cell lysate. Monitoring PKC activity in single living cells is not possible with such experiments. This thesis presents a …
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