Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"intestinal organoid"”.
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An integrated system for selection and manipulation of single cells in culture
… This system was then applied to study intestinal organoid formation. Specifically, current protocols for the production of intestinal organoids suffer from low yield, with only ~12% of the highly heterogeneous nascent spheroids successfully maturing into intestinal organoids. It was …
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Development of a new screening system for the identification of RNF43-related genes and characterisation of other PA-RING family members.
… which combines CRISPR/Cas9 technology with 3D-intestinal organoid culture for the identification of novel molecular interactors of RNF43. Overall, this study and the technology developed provide a tool to enable the detailed description of the mechanism of action of RNF43, which is important …
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Modeling colorectal cancer initiation using human iPSC-derived intestinal organoids
… pluripotent stem cell (hiPSC)-derived human intestinal organoids (HIOs) are increasingly utilized as in-vitro models for studying colorectal cancer (CRC). Prevalent oncogenic drivers in CRC include mutations in EGFR (L858R), KRAS (G12D) and CTNNB1 (S33C) which are known to contribute to …
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Investigating the movement of intestinal intraepithelial lymphocytes using an organoid co-culture system
… intestine, which is lined by a single layer of intestinal epithelial cells, is the main site of nutrient absorption. It is continuously exposed to food antigens, and also several bacteria and microorganisms. Intestinal intraepithelial lymphocytes (IELs) are T cells that are interspersed between …
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Characterisation of human enteroendocrine cells in organoid models
… endocrine cells scattered throughout the gastrointestinal epithelium that release hormones in response to a wide range of luminal and systemic signals. This thesis focused on characterising the secretory mechanisms of three important gut hormones: glucagon-like peptide 1 (GLP-1, which has potent …
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Modelling the interactions of Salmonellae with the human host using stem cell-derived intestinal organoids and macrophages
… interactions has been developed; known as “organoids” or human intestinal organoid systems (iHO). These can be produced from human induced pluripotent stem cells (hiPSCs), or from primary intestinal tissue, and once matured, harbour differentiated enterocytes and secretory cells such as …