Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"internal transcribed spacer (ITS)"”.
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Phylogeographic patterns in three South African forest mosses
… of forest patches, two molecular markers, internal transcribed spacer (ITS) nuclear rDNA and trnL-F cpDNA were employed.
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Evaluating oxalate-degrading Lactobacillus spp. for their ability to be used as probiotics in the treatment of kidney stone disease
… of the 16S rRNA gene and the 16S-23S rRNA internal transcribed spacer (ITS) region. The strains were identified as follows; Lactobacillus gasseri 7(3), L. gasseri 17(4), Lactobacillus reuteri 17(7) and L. reuteri 16(9). Their probiotic characteristics were also evaluated.
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Cyperaceae tribe Cyperae : phylogenetic relationships and evolutionary patterns of diagnostic characters
… of plastid DNA (rbcL, trnL-F intergenic spacer, and rps16), nuclear ribosomal DNA (internal transcribed spacer ITS) sequences and morphological data. From both analyses, tribe Cypereae were resolved into a very strongly supported clade (Posterior probability =98%; Bootstrap= 100%) …
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A multilocus phylogeny of Hericium fungi in Canada and their production of Erinacine A
… phylogenies of the genus Hericium based on the internal transcribed spacer (ITS) region struggle to support monophyly at the species level, hindering biochemical research efforts. Using concatenated sequence data from ITS, the large ribosomal subunit (LSU), translation elongation factor 1-alpha …
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Isolating and Identifying Fungal Endophytes from Roots of Rare Orchid Cypripedium arietinum in Grand Sable Dunes, Michigan
… conserved 5.8s rDNA and the highly variable internal transcribed spacer (ITS) regions on either side of it. DNA was sequenced and sequences were identified using NCBI BLAST. Of the 36 DNA samples 27 were positively identified. <em>Phialocephala fortinii</em> and <em>Leptodontidium …
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Diversity of the marine cyanobacterium Trichodesmium : characterization of the Woods Hole culture collection and quantification of field populations
… two clades based on sequences from 16S rDNA, the internal transcribed spacer (ITS), and the heterocyst differentiation gene hetR. Clade I contained Trichodesmium erythraeum and Trichodesmium contortum, and clade II contained Trichodesmium thiebautii, Trichodesmium tenue, Trichodesmium …
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Mentha (Lamiaceae) Phylogenetic Analysis Using Chloroplast TRNL-TRNF and Nuclear Ribosomal DNA ITS Sequences
… is considered challenging systematically due to its high incidence of polyploidy, diverse morphology, variation in base chromosome number, and frequent interspecific hybridization. Our objectives were to test the monophyly of Mentha and each of its traditionally recognized sections, assess …
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Nymphaea odorata (Water-lily, Nymphaeaceae): Analyses of molecular and morphological studies
… odorata. Molecular analyses of the nuclear internal transcribed spacer (ITS) region, the chloroplast trnL-F region, and inter-simple sequence repeat (ISSR) markers determined the variation present between and within two species of Nymphaea. The ITS region resulted in a phylogeny depicting …
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Assessment of species boundaries of the Moringa Ovalifolia (Dinter & A. Berger) in Namibia using nuclear its DNA sequence data
… in the tropical and subtropical regions. For its size, this genus is one of the phenotypically varied group of angiosperms whose members are mostly sought after for nutritional, nutraceutical and industrial uses. The aim of this study was to assess the species boundaries of Moringa ovalifolia …
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A Systematic Revision of Bakeridesia Hochr. (Malvaceae)
… several approaches were used to investigate its species boundaries and phylogenetic relationships. First, phylogenetic analyses of Internal Transcribed Spacer (ITS) sequences provided strong evidence that Bakeridesia is polyphyletic if circumscribed to include the Brazilian subgenus Dipteron. …
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Diversity of fungi associated with dieback of Ziziphus Mucronata in Limpopo Province, South Africa
… The tree is considered important because of its useful parts for various purposes. For example, leaves of this tree can be consumed as a vegetable by humans and wild animals such as antelopes and baboons feed on them. Fruits from Z. mucronata are edible and nutritious to both human and wild …
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Fungal Biofilm Colonization and Succession on Artificial Reefs in the North-Central Gulf of Mexico
… to determine fungal presence by amplifying the internal transcribed spacer (ITS) region of fungal ribosomal DNA. Terminal fragment length polymorphism (T-RFLP) analysis of the ITS region was used to infer variation in fungal community structure. Fungal species were further characterized by …
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Diversity of the marine cyanobacterium Trichodesmium : characterization of the Woods Hole culture collection and quantification of field populations
… two clades based on sequences from 16S rDNA, the internal transcribed spacer (ITS), and the heterocyst differentiation gene hetR. Clade I contained Trichodesmium erythraeum and Trichodesmium contortum, and clade II contained Trichodesmium thiebautii, Trichodesmium tenue, Trichodesmium …
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Molecular phylogenetic studies in nyctaginaceae: patterns of diversification in arid North America
… an intensively sampled phylogeny based on internal transcribed spacer (ITS) and nitrate reductase (NIA) sequences provides new insights into relationships among species in the genus, and identifies a clade of annual species centered in the Sonoran Desert. Phylogeographic patterns are …
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Evaluation of Downy Mildew (Peronospora farinosa f.sp. chenopodii) Resistance among Quinoa Genotypes and Investigation of P. farinosa Growth using Scanning Electron Microscopy
… with polymerase chain reaction (PCR) using ITSP primers designed from the internal transcribed spacer (ITS) region of the pathogen. Scanning electron microscopy (SEM) also revealed that P. farinosa penetrated stomata via appressoria, secreted extracellular matrices during sporangia …
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Identification of fungal species causing powdery mildew on cucurbits, determination of the genetic and pathogenic variations of the fungi and their sensitivity to major powdery mildew fungicides
… mildew is an annually-occurring disease of cucurbits, which causes yield losses up to 50% in Illinois and worldwide. Podosphaera xanthii and Golovinomyces cichoracearum are known as the causal agents of cucurbit powdery mildew. No reported investigations have been conducted on the species …
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Molecular and morphological phylogenetic analyses of Euphorbia L. (Euphorbiaceae) with an emphasis on Southern African representatives
… and on sequences of the nuclear ribosomal internal transcribed spacer (ITS) and chloroplast psbA-trnH intergenic spacer. Fifty-one species of Euphorbia and four outgroups from Monadenium and Ciutia were sampled. Matrices were analysed using parsimony and maximum likelihood methods. Separate …
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Prevalence of human African trypanosomiasis and its associated risk factors in Itezhi-tezhi district of Zambia.
… namely packed cell volume (PCV), microscopy, internal transcribed spacer (ITS)/serum resistance antigen (SRA) polymerase chain reaction (PCR) and SRA loop mediated isothermal Amplification (SRA-LAMP). Structured questionnaires collected data on risk factors as well as the district’s health …
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Systematics and reproductive biology of the genus Morus L. (Moraceae)
… North, Central and South America. Despite its broad distribution and economic importance, it has received little attention from systematic botanists. Two species of this genus, the native M. rubra and the exotic M. alba, co-occur in eastern North America including the Flint Hills region of …
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Mycorrhizal fungus communities of Douglas-fir (Pseudotsuga menziesii) seedlings and trees: effects of proximity to residual trees
… PCR-RFLP, and sequence analysis of the internal transcribed spacer (ITS) and other ribosomal RNA genes.Seedlings <6 m from residual trees had significantly higher root to shoot biomass ratios. Stem growth was greater for seedlings <6 m from trees at a site with minimal understory, but …
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