Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"intermediate filaments"”.
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Diverse Roles Of Nuclear Intermediate Filaments In Proliferating Cells
… studies the contrasting role of two nuclear intermediate filaments - Lamin A and Lamin B1 in the proliferating cells of embryonic hearts and cancer. Lamin B1 is upregulated together with proliferation genes in at least 15 cancers curated in The Cancer Genome Atlas (TCGA), whereas Lamin A …
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Impact of Vimentin Intermediate Filaments on 3D Multicellular Collective Behavior
Vimentin, a type III intermediate filament, is an understudied component of the cytoskeletal system. However, in recent studies we can see its structural and mechanical properties aid in a cell's survival and migration. It forms a hyperelastic network and works synergistically with actin and …
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Structural and mechanical properties of intermediate filaments under extreme conditions and disease
Intermediate filaments are one of the three major components of the cytoskeleton in eukaryotic cells. It was discovered during the recent decades that intermediate filament proteins play key roles to reinforce cells subjected to large-deformation as well as participate in signal transduction. …
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Developmental Appearance of Phosphorylated Intermediate Filaments in Nuclei of Glioma and Neuroblastoma Cells
… vaguely known. Investigations into the role of intermediate filaments (IF) in the nucleus have primarily focused upon lamins, however some studies have suggested that there may be a role for non-lamin intermediate filaments in the nucleoskeleton. Recent studies of glioma and neuroblastoma cell …
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An Immunochemical Analysis of Alcoholic Hyaline and its Relationship to Keratin Intermediate Filaments
<p>Alcoholic Hyaline (AH) and keratin intermediate filaments possess ultrastructural, biochemical and immunochemical similarities. This study compares human AH, human stratum corneum keratin (HSCK) and normal human liver cytokeratin (HCK) by quantitative immunochemical means, using polyclonal as …
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Novel Intermediate Filaments in the Nuclear Envelope: Molecular Cloning and Analysis of Lamins A and C
… that identify them as members of the family of intermediate filament proteins. Both lamins share an approximately 360 amino acid residue domain of α-helical conformation that, in turn, shows primary and secondary structural homology with the α-helical rod domain of the intermediate filament …
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Role of Gigaxonin in the Regulation of Intermediate Filaments: a Study Using Giant Axonal Neuropathy Patient-Derived Induced Pluripotent Stem Cell-Motor Neurons
… GAN pathology is characterized by aggregates of intermediate filaments (IFs) in multiple tissues. Disorganization of the neuronal intermediate filament (nIF) network is a feature of several neurodegenerative disorders, including amyotrophic lateral sclerosis, Parkinson's disease and axonal …
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Toxicity of microcystin-LR in hepatocytes and non-hepatocytes in vitro
… to excessive phosphorylation and collapse of intermediate filaments (IFs) in hepatocytes. MCLR was used as a probe to understand the mechanism of action of this toxin in hepatocytes and non-hepatocytes.
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First-passage time analysis of particle transport in the cytoplasm
… then used to investigate the effect of vimentin intermediate filaments (VIFs) on cytoplasmic transport. We find that VIFs significantly inhibit the displacement of objects in the cytoplasm.
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Biomechanical regulation of T cells: The cytoskeleton at the nexus of force and function
… their cytoskeleton, a dynamic network of protein filaments, consisting of actin, microtubules, and intermediate filaments, which provides structure, facilitates movement, and regulates intracellular transport. While the biochemical aspects of T cell function have been well-studied, recent advances …
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Effects of mitochondrial dysfunction on neurofilament turnover and distribution in human neuroblastoma cells
… including α-synuclein, ubiquitin and neurofilaments (NFs). NFs, the intermediate filaments expressed in neuronal cells are responsible for the maintenance of axonal structure. Although NFs were the first proteins identified in LBs their role in PD pathogenesis has not been fully explored. …
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Acute Nitrate Exposure Causes Proteomic Changes Consistent with the Regulation of Reactive Oxygen and Nitrogen Species
… (lipocalin) and cytoskeletal arrangements (intermediate filaments and a gelsolin-like adseverin). The most parsimonious explanation for these changes in protein expression assumes that C. gigas reduces nitrate to nitrite and nitric oxide, which reacts with superoxide anions to form the very …
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Cancer Protrusions on a Tightrope - Suspended Fiber Platform Reveals Protrusion Dynamics Independent of Cell Migration
… DBTRG-05MG. Furthermore, we find that vimentin intermediate filaments localize within 70% of mature protrusions, which normally form on larger diameter fibers. Additionally, protrusion lengths fluctuate continuously until the protrusion is either terminated or stabilized, and occasionally …
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Identification of altered Ras signaling and intermediate filament hyperphosphorylation in giant axonal neuropathy
… organization of cytoskeletal proteins called intermediate filaments (IFs), which normally provide strength and support for the overall cell structure. The irregular IF structure in GAN patient neurons leads to a progressive loss of motor skills in children and subsequent death in adolescence. …
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Cyclic AMP and the Induction of Reactive Astrogliosis
… recognize a phosphoepitope found on the nuclear intermediate filament, lamin, as well as the cytoplasmic intermediate filament characteristic to mammalian astrocytes, glial fibrillary acidic protein (GF AP), suggesting that mAB Jl-31 is an appropriate marker for astrocytes entering the reactive …
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Mechanical integration between cytoplasmic networks during cytokinesis
… cytoplasmic components – microtubules, F-actin, intermediate filaments, endoplasmic reticulum, mitochondria, and cytosol – are imaged, in order to characterize flows involved in nuclear positioning and cytoplasmic organization. While the system had been conceptualized as a solid network of …
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IDENTIFICATION OF CANCER- AND METASTASIS-RELATED EPIGENETIC DYNAMICS USING PATIENT-DERIVED ORGANOIDS
… pathways relative to tissue development and intermediate filaments organization were enriched. As trans modulators of these enhancers, we identified transcription factors belonging to the Wnt/β-catenin pathway and AP-1 complex. We also observed epigenomic and transcriptomic inter-metastases …
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Structural studies of the in vitro assembly of tau and α-synuclein amyloids
… composed of amyloids. Cryo-EM studies of amyloid filaments isolated from human brains have revealed that specific conformers of tau and α-synuclein are associated with different diseases. These findings suggest that specific molecular mechanisms underlie the formation of filaments in the different …
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Multiscale modeling and simulation of deformation and failure mechanisms of hierarchical alpha-helical protein materials
… here is generally applicable to other protein filaments that feature a serial array of domains that unfold under applied strain. Although simple, our coarse-grained cell model agrees well with experiments and illustrates how the multiscale approach developed here can be used to describe more …
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GFP- coated microparticles to quantify and compare wild- type desmin with known desmin mutations in human heart disease via quantitative live-cell fluorescence Imaging
… (p.D312A), desmin is an essential protein of the intermediate filaments in myocytes, and its mutations are associated with many cardiac and skeletal muscle disorders. C2C12 myoblast cells were transiently transfected with tagged desmin constructs carrying enhanced green fluorescent protein (EGFP) …
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