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Showing 1 to 8 of 8 for “"inteins"”.
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Split Inteins: From Mechanistic Studies to Novel Protein Engineering Technologies
<p>Inteins are auto-processing protein domains that carry out a post-translational process known as protein splicing. This process is characterized by excision of the intein (intervening protein) domain from within a larger polypeptide sequence with concomitant ligation of the flanking extein ( …
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A Selection for Intein Splicing inPhage Assisted Continuous Evolution
… goal in chemical biology. Trans-splicing inteins fulfill a crucial niche in this area by enabling post translational splicing of separate polypeptides, introduction of post translational modifications, and other chemistry on the amide backbone. This has motivated engineering and evolution …
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ADVANCED AAV-MEDIATED LIVER-DIRECTED GENE THERAPIES FOR HAEMOPHILIA A AND MUCOPOLYSACCHARIDOSIS TYPE VI
… trans-splicing (PTS) strategy utilizing split inteins. PTS is a natural mechanism employed by organisms across all domains of life to reconstitute large proteins from shorter precursors. Split inteins facilitate this process by selfexcising from the host protein in an energy-independent manner. …
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Mobile genetic elements in Coxiella burnetii: Friends, foes or just indifferent?
… intact and presumably functional. Similar inteins were found in two extremophilic bacteria (Alkalilimnicola ehrlichei and Halorhodospira halophila) that were found to be distantly related to Coxiella. Intein splicing appears to be a slow process, making it possible that before the intein is …
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Mobile genetic elements in Coxiella burnetii: Friends, foes or just indifferent?
… intact and presumably functional. Similar inteins were found in two extremophilic bacteria (Alkalilimnicola ehrlichei and Halorhodospira halophila) that were found to be distantly related to Coxiella. Intein splicing appears to be a slow process, making it possible that before the intein is …
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THE CRISPRATOR: A FEEDBACK CONTROLLER IN MAMMALIAN CELLS FOR ROBUST PERFECT ADAPTATION
… presented very recently using mRNA pairs and inteins. In this thesis, I implemented the CRISPRaTOR, an Integral Controller based on the interaction between a CRISPR/Cas9-derived transcription factor and the recently discovered anti-CRISPR proteins, demonstrating its capability to achieve …
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Molekularbiologische Gewinnung von RNase 40-124 Fragmenten zur Synthese von einheitlichen Glycoproteinen durch native chemische Ligation
… rückgefaltet. Trotz einer fast vollständigen Inteinspaltung konnte das oxidationsempfindliche und möglicherweise unlösliche Zielpeptid RNase 40-124 B nicht isoliert werden. Deshalb wurden die sieben Cys von B vor der Rückfaltung und Inteinspaltung durch gemischte Disulfide geschützt. Die …
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Structural and Biophysical Characterisation of Denatured States and Reversible Unfolding of Sensory Rhodopsin II
… protein complexes. Naturally occurring split inteins can mediate spontaneous trans-splicing both in vivo and in vitro. Previous studies have demonstrated successful assembly of proteorhodopsin from two separate fragments consisting of helices A–B and helices C–G via a splicing site in the BC …