Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"integration site"”.
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Site Directed Mutagensis of Bacteriophage HK639 and Identification of Its Integration Site
… this newly identified phage we generated site directed mutations and identified where the phage integrates into the chromosome of its bacterial host.
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Urban Brownfield Integration – Site Planning and Design Implementation of the Baltimore Community Center
… many cities were left with large, waterfront sites, often contaminated with chemicals and empty industrial buildings. These waterfront sites – designated "Urban Brownfields" – became important potential centerpieces to the revitalization of the cities where manufacturing facilities were slowly …
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Genomic Analysis And Engineering Of Chinese Hamster Ovary Cells For Improved Therapeutic Protein Production
… such as cellular growth, metabolism, and the integration site of the gene encoding the protein, influence the resulting culture productivity and quality of the protein produced. In this thesis, multiple aspects of the relationship between integration site and resulting cell line behavior were …
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Multi-Pronged Approach to Understanding the Toxicity Associated with CAR T-Cell Therapy
… transcriptomic, and functional strategies. Virus integration is essential for CAR expression, yet the biological consequences of integration are not yet fully understood. Integration site profiling therefore provides a means to assess whether certain patterns are associated with functional …
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Integration Sites in the Persistence of Latent HIV-1
… herein elucidates the contribution of proviral integration site to HIV-1 latency and the maintenance of the replication-competent, or intact, reservoir. Using an innovative single-cell sequencing technique that provides paired proviral sequence and integration site information, as well as …
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Interactions between mobile genetic elements and their bacterial hosts
… some of these effects. Finally, I evaluated the integration site selection of Tn916 in the B. subtilis chromosome, identifying several hundred unique AT-rich insertion sites, one of which is a “hot spot” for integration. I found that a host nucleoid-associated protein does not influence …
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Role of Wnt signaling in proliferation of human bone marrow-derived mesenchymal stem cells
… during in vitro expansion. Wingless-related MMTV integration site (Wnt) is well known signaling molecules that implicated in mesenchymal differentiation during development, but the role in proliferation of hMSCs is not well known. In this study, we investigated the effect of Wnt signaling …
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Using a Mammalian Virus to Create Plants for Site-Specific Transgene Insertion
A novel strategy for site-specific DNA transformation of plants has been proposed and the first component of the system developed. The proposed method overcomes the limitations of current techniques by providing a specific integration site for the insertion of transgenes using features of the …
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Insights into the clonal inventory of gene corrected human SCID-XI hematopoiesis
The semi-random nature of retroviral integration introduces a stable molecular marker into the cellular genome in the form of a genomic-proviral fusion sequence which can be passed on unmodified to all the progenies of a transduced cell. Retroviral integration site analysis provides a powerful …
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Exploiting Lentiviral Integrations to Profile Chromatin
… ChILI-seq (Chromatin Interactions by Lentiviral Integration Sequencing), an antibody-free and sequence-independent method to profile the genomic localisation of proteins, as well as DNA and histone modifications. ChILI-seq exploits the dependency of the lentiviral integrase on LEDGF (Lens …
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Algorithms for E. coli genome engineering
… on the genomic regions that are targeted (integration sites). In this project we explored ways of identifying what makes a site well suited for lambda red genome engineering. We wanted to explore whether we can eventually predict the "goodness" of an integration site using an algorithm. Our …
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Identification of Factors Involved In Dna Methylation of Cpg-Island-Promoters
… the first part of our project, we established a site-specific integration system with enforced local transcriptional repression in colorectal cancer cells and monitored the occurrence of de novo DNA methylation in exogenous fragments containing a CGI-promoter and repetitive elements. Initial de …
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Differential modulation of HIV-1 capsid uncoating kinetics revealed by a novel single-particle uncoating assay
… with the nuclear pore complex, and directs integration site targeting. To achieve all of these functions, the capsid disassembles, in a process referred to as uncoating, with temporal and spatial precision. HIV has evolved to exploit a network of host cofactors to regulate capsid stability …
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Latency, Expression and Splicing During Infection With HIV
… genomic and epigenetic features to HIV-1 integration site data for latent and active provirus from five cell culture models. Latency was associated with chromosomal position within individual models. However, no shared mechanisms of latency were observed between cell culture models. These …
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Molecular Mechanisms of HIV-1 Infection: Viral and Host Determinants in Transmission and Pathogenesis
… in neonatal vs. adults cells, 468 HIV-1 integration sites were characterized in the T-lymphocytes and monocyte-derived-macrophages from 5 donors of infant and adult blood. Several functional classes of genes were identified by gene ontology to be over represented, including genes for …
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Transcriptional regulators in stem cell biology
… B-cell-specific Moloney murine leukemia virus integration site 1 (BMI1). BMI1 promotes stemness and tumorigenesis in many tissues through both transcriptional repression of the Cdkn2a locus and other functions. Notably, BMI1 supports melanoma progression by activating epithelial-mesenchymal …
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Pim Kinase Inhibition: Single Agent and Combination Approaches In B-Cell Lymphoma
<p>Proviral integration site for Moloney murine leukemia virus (Pim) kinases are Ser/Thr/Tyr kinases. They modulate B-cell development but become oncoproteins and promote cancer development once overexpressed. Containing three isoforms, Pim-1, -2 and -3 are known to phosphorylate various substrates …
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Investigating the genomic distribution and potential contribution of retrotransposable elements in relation to their potential impact on genome function and predisposition to human diseases.
… are likely more representative of RTEs preferred integration site. The genomic distribution of the curated RTEs was compared with the distribution of ancient RTEs that are fixed in the human genome to hypothesise the likely effect of new RTE insertions. Non-reference insertions were found at …
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Different Genetic Ways for Conditional Gene Regulation in the Mouse Brain
… promoter was used, which is known to be highly integration-site dependent, lines were obtained which all differed in tTA expression level, expression pattern and ontogenetic profile in the central nervous system (CNS). These mice are a valuable tool for studies on physiological functions of …
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A NOVEL METHOD TO INTERFERE WITH GENE EXPRESSION IN MICE
… the dependence of expression on the chromosomal integration site. A good correlation between EGFP activity and siRNA accumulation in organs of F1 mice was found as evidenced by Northern blot hybridisation. Despite the general low efficiency of transgenesis the down regulation of HNF4gamma gene in …
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