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Showing 1 to 11 of 11 for “"integration host factor (IHF)"”.
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The Integration and Excision Mechanisms of the Bacteroides Conjugative Transposon CTnDOT
… partial-purified CTnDOT Int protein, an in vitro integration system was developed and a host factor(s) from Bacteroides cell extract was shown to be important for integration. The CTnDOT Int protein catalyzes the in vitro integration reactions by a coupling sequence mechanism similar to the in …
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Biochemical and genetic analysis of nucleic acid-protein interactions
The integration host factor (IHF) of Escherichia coli is a small, sequence-specific DNA-binding protein. The specific and nonspecific DNA binding constants were determined by gel mobility shift assays. The binding constant of IHF for the H$\sp\prime$ site in $\lambda$ attP is 6.8 $\times$ 10$\sp8$ …
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An Analysis of Bacteriophage Lambda Site-Specific Recombination Using IHF Binding Site Mutants
Integration Host Factor (IHF) is an Escherichia coli protein required for integration and excision of bacteriophage lambda. Mutations of the three sites to which this protein binds within the lambda attachment site were constructed using oligonucleotide-directed site-specific mutagenesis and were …
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Characterization of the Gifsy-1 Phage Excisionase
… is a temperate bacterial virus that infects its host, Salmonella enterica serovar Typhimurium. During lysogeny, the phage integrates site-specifically into a single bacterial attachment sequence in the Salmonella chromosome. The reaction involves recombination between the phage attachment …
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Integration Host Factor and Its Interaction With the H-Prime Site of Bacteriophage Lambda
Integration Host Factor (IHF) is a sequence-specific DNA-bending protein of Escherichia coli that recognizes the DNA through contacts made to the backbone and the minor groove. IHF binds specifically to the H$\sp\prime$ site, $5\sp\prime$-TAAAAAAGCATTGCTTATCAATTTGTTGCAACGA-$3\sp\prime$, with a $\rm …
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Studies On The In Vitro Transposition Of Bacteriophage Mu Dna
… was dependent on an additional E. coli protein factor which we named supercoiling relief factor (SRF). This protein was shown to be integration host factor (IHF). Low levels of IHF also reduce the amount of HU and Mu A required for the reaction and at high concentrations of IHF, HU is not …
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DNA-Protein Interactions in f1 DNA Replication
… contains three binding sites for the E. coli integration host factor (IHF). Both activator proteins bend the DNA sequence to which they bind, implying that together they wrap the origin DNA into a higher order structure that is active in initiation. The replication initiator protein of …
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Cooperative Assembly of Terminase and Integration Host Factor at the Packaging Initiation Site of Bacteriophage Lambda
… this thesis, I interrogate the role of E. coli integration host factor (IHF) in mediating the site-specific assembly of bacteriophage lambda terminase at its cognate DNA site, cos, which serves as the packaging initiation site. IHF binds to an I-element within cos and introduces a strong bend in …
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Molecular mechanisms underlying regulation of type III secretion system upstream of the master regulator HrpL in Erwinia amylovora
… previously demonstrated that HrpL, an ECF sigma factor, is the master regulator of hrp-T3SS. Recently, it is reported that expression of hrpL is under the control of sigma 54 complex, including σ54 (RpoN), its modulation protein YhbH and σ54-enhancer binding protein HrpS. In this study, we …
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The role of Bacteroides Host Factor A in CTnDOT integration and Holliday junction resolution
… normal conditions, Bacteroides spp. benefit the host by breaking down complex polysaccharides. However, when the gut is punctured, these species may escape to cause abscesses or infections within the blood. In the past, tetracycline was used as a treatment for these infections. However, …