Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 53 for “"intact cells"”.
-
Developing a nanobody based technique to investigate interactions between signalling proteins in intact cells
Abstract not available. Please refer to PDF.
-
Monitoring apoptosis in intact cells by high-resolution magic angle spinning 1H NMR spectroscopy
… non-invasive monitoring of apoptosis in intact cells could contribute towards the characterisation of drug efficacy and hence drug discovery. A known metabolic marker of apoptosis is a notable increase in proton NMR resonances associated with lipids stored in lipid droplets (LDs). …
-
Systematic Survey of the Plasmodium falciparum Hemoglobin Catabolic Pathway in Intact Cells Using the Digestomics Approach
… underpinnings of this catabolic process in intact cells remain poorly understood. This gap in knowledge directly results from the non-linear nature of protein catabolism in living cells. Proteolytic cascades, such as the Hb proteolytic pathway in Plasmodium parasites, produce large …
-
Structure-Function Studies of the cAMP-Dependent Protein Kinase <i>In Vitro</i> and in Intact Cells
… also determined that Cγ expressed in Sf9 insect cells, like Cα expressed in mammalian cells, is phosphorylated at T197 and S338 and the modification at T197 is important to the function of both isoforms. The third approach sought to characterize the kinetic mechanism of PKA-Cγ through …
-
Characterization of the phosphorylation of thylakoid membrane proteins from cyanobacterium synechococcus sp. PCC 6301 in light state 1 and light state 2
… and soluble proteins rapidly isolated from intact cells in state 1 and state 2 was investigated. 77 K fluorescence emission spectra revealed that rapidly isolated thylakoid membranes retained the excitation energy distribution characteristic of intact cells in state 1 and state 2. …
-
Excitation-Contraction Coupling: The Effect of Calcium Channel Antagonistic Drugs on Reprimed and Inactivated Skinned Skeletal Muscle Fibers
… fibers are analogous to K contractures on intact cells.
-
Cytoplasmic RNA-dependent RNA-synthesis in maturing chicken erythrocytes.
… The product synthesized after incubation of intact cells in the presence of [³H]-uridine and actinomycin D was shown to be very similar with respect to S-value and the ratio of UMP/uridine incorporated. This similarity suggests the presence of a ribosome bound RNA-dependent RNA polymerase …
-
Studies on the Degradation of Dihydrofolate Reductase and Other Intracellular Proteins by Mammalian Cells
<p>The mechanisms by which mammalian cells degrade intracellular proteins have been investigated. Radioactively labelled proteins of rat hepatoma tissue culture cells were incubated in cell-free systems in an attempt to identify the degrading processes. Although proteins in intact cells are …
-
Regulation of Glycogenolysis in Anaerobic Skeletal Muscle
… of anaerobic metabolism was demonstrated in intact tissue. Force-independent ATP utilization, primarily the Ca 2+ pump of the sarcoplasmic reticulum, preferentially uses ATP derived from glycogenolysis. ATP produced by creatine kinase is used mostly by force-dependent ATP consuming processes, …
-
Therapeutic Potential of Prmt5 and Mat2A As Synthetic Lethal Targets In Mtap-Deficient Gbm Tumors
… (MTA). As a result, <em>MTAP</em>-deleted cells cannot metabolize MTA, leading to the accumulation of MTA. High levels of MTA in <em>MTAP</em>-deleted cells partially inhibit the activity of protein arginine methyltransferase 5 (PRMT5), making these cells sensitive to PRMT5 and MAT2A …
-
Regulation and role of the LKB1-AMPK pathway
… 100-fold.<br/><br/>Using cell-free systems and intact cells, the regulation of Thr172 phosphorylation and AMPK activity by AMP, ADP and ATP was re-investigated. AMP promoted Thr172 phosphorylation by LKB1 but not by CaMKKß, while ADP had no effect on Thr172 phosphorylation by either upstream …
-
Tracing viable bacteria in Wadden Sea sediments using phospholipid analysis
… Special emphasis was placed on the analysis of intact phospholipids by HPLC-ESI-MS/MS, because phospholipids appear to be suitable biomarkers to trace viable bacteria. The specificity of phospholipids as biomarkers was tested by investigating the lipid composition of 30 different strains of …
-
Cellular responses to external threats probed by super-resolution microscopy
… biological systems. Viral infection in mammalian cells triggers the immune response, a cascade of antiviral signaling proteins. For double stranded RNA (dsRNA) virus, the innate immune response starts from retinoic acid-inducible gene-I (RIG-I) protein which is a dsRNA sensor. For certain types of …
-
The relationship between two forms of glycogen phosphorylase in Dictyostelium discoideum
… the 104 kd protein. Cyclic AMP perturbation of intact cells caused induction of both phosphorylase ’a' activity and the appearance of the 104 kd protein. lmmunotitration data suggest that the ’a’ form accumulates due to de novo protein synthesis, although this result must be interpreted with …
-
Characterization of Ligand-Induced Conformational Changes in the EGF Receptor
… evidence of these structural dynamics in intact cells has been lacking. This thesis describes the characterization of sequential ligand-induced conformational changes in the EGF receptor in live cells in real time using luciferase fragment complementation imaging. We find that these …
-
Development and application of a gene transfer system in Clostridium perfringens
… electroporation-induced transformation of intact cells of C. perfringens 3624A-Rif$\sp{\rm r}$/Str$\sp{\rm r}$ with plasmids pAM$\beta$1 and pHR106 resulted in transformation efficiencies of 1.4 $\times$ 10$\sp2$ and 1.2 $\times$ 10$\sp3$ transformants/$\mu$g DNA, respectively. An …
-
A screen for phosphatidylinositol 3,4-bisphosphate and other 3-phosphoinositide effector proteins
… recruitment of target proteins to membranes of intact cells, selectively enriched in PtdIns(3,4)P2. In addition, this screen utilises stable isotope labelling with amino acids in cell culture (SILAC) to differentially label cells stimulated in the absence and presence of the PI 3-kinase …
-
Applications of fluorescence detected sedimentation I Studying the thermodynamic impact of valence in non-ideal solutions II High affinity interactions and biological media
… about diffusion and assembly in the context of intact cells. New findings in this area will help to build upon what is already known about macromolecular crowding and aid in the refinement of current crowding theory. Examples of the applications listed above will be presented highlighting a …
-
Aspectos da biologia e ecologia de Callibaetis guttatus Navás, 1915 (Insecta: Ephemeroptera: Baetidae) em alagados temporários da Restinga de Maricá, Estado do Rio de Janeiro, com considerações taxonômicas
… stages of decomposition, vegetable fibres with intact cells, and many algae. The alimentary Pattern probably varied according to the different limnological phases of the marsh. During the decrease of water level the proportion of filamentous algae increased and vegetable fibres decreased in the …
-
Small molecule inhibitors: novel probes of progesterone receptor action
… pocket and selectively inhibit PR activity in intact cells. These small molecules inhibit PR-mediated induction of the mouse mammary tumor virus (MMTV)-luciferase reporter and endogenous alkaline phosphatase activity in T47D cells with IC50s in the low micromolar range. They inhibit PR with …
Page 1 of 3