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Showing 1 to 6 of 6 for “"insertional mutant"”.
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Seed germination, kanamycin sulfate selection, and the influence of nitrogen treatments on an insertional mutant population of Fragaria vesca
… efficient methods of generating unique Ac/Ds insertional mutants in a population of Fragaria vesca, various methods of seed germination, kanamycin screening, and the effects of varying nitrogen fertilization on diploid strawberry have been examined. Seed germination was improved to 42% in B5 …
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N-Acylethanolamine Metabolism During Seed Germination: Molecular Identification of a Functional N-Acylethanolamine Amidohydrolase
… Arabidopsis cDNA as a FAAH homologue. Two T-DNA insertional mutant lines with disruptions in the Arabidopsis NAE amidohydrolase gene (At5g64440) were identified. The homozygous mutant seedlings were more sensitive than the wild type to exogenously applied NAE 12:0. Transgenic seedlings …
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Improved regeneration and Agrobacterium-mediated transformation of wild strawberry (Fragaria vesca L.)
… transformation protocol to generate an insertional mutant population to support the justification of F. vesca as a model organism for rosaceous crops. The transformation techniques described by Alsheikh et al. (2002) and Oosumi et al. (2005) were modified and applied to a range of …
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GENETIC NETWORK CONTROLLING FEMALE GAMETOPHYTE AND EMBRYO DEVELOPMENT IN ARABIDOPSIS THALIANA
… sac. AGL23 was isolated after a large T-DNA insertional mutant screening in type I MADS-box genes. The results presented in chapter 1 indicate that AGL23 is necessary for female gametophyte and embryo development. Indeed, in agl23 mutant the functional megaspore is not able to undergoes …
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Isolation and characterization of two closely related beta-carbonic anhydrases of Chlamydomonas reinhardtii
… for CAH7 and CAH8 are discussed. Previously, insertional mutants were generated to be able to isolate bicarbonate-transporters and other proteins that might be essential for a functional CCM. One of the generated insertional mutants is slc211, a mutant that requires high carnon dioxide for …
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Investigating the molecular basis of vitamin B12 uptake in Chlamydomonas reinhardtii
… other proteins important in B12 uptake, using insertional mutagenesis. A reporter line was made with a paromomycin-resistance marker under control of a B12-repressible promoter, so that it showed repression of growth in paromomycin under high media vitamin B12 concentrations (+P+B12). As this …