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Showing 1 to 13 of 13 for “"insertion sequence"”.
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Insertion sequence IS1141: discovery, characterization, and association with Mycobacterium intracellulare colonial variation
… containing copies of the putative element were sequenced and a region 1596 basepairs in length with 23 basepair imperfect inverted repeats was designated as insertion sequence IS1141. IS1141 is the first insertion sequence identified in M. intracellulare. Data base searches using open reading …
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Understanding Genomes Through Engineered Structural Variation
… ability of prime editors to insert recognition sequences for recombinases at high throughput to engineer genomes at an unprecedented scale. In the process, I made discoveries about the biology of genome engineering, structural variation, and gene regulation. I first outlined the determinants of …
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Mechanisms of Translational Control
… basis of Sec stop codon suppression and insertion, as well as the phenotypic consequences of perturbations to this process. Additionally, we explore how ribosome-binding antibiotics, particularly the pleuromutilin and aminoglycoside classes, affect translation initiation and termination, …
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Regulation of blaFOX-5b Expression Within Two Different Genetic Backgrounds: Klebsiella pneumoniae and Escherichia coli
… located upstream of blaFOX-5b within the plasmid sequence were required for high level expression to confer resistance. Therefore to address the hypothesis of this thesis research, the promoter of a novel plasmid-encoded P-lactamase gene, blaFOx-5b was located and the genetic elements responsible …
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An evaluation of DNA fingerprinting methods for subtyping Salmonella
… Salmonella utilizing probes from ribosomal DNA, insertion sequence IS200, or random sequences has been reported. Ribotypes detected with ribosomal probes have generally been shared among different serotypes, whereas IS200 profiles have tended to be more serotype-specific. AP PCR and rep-PCR …
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Genetic and molecular characterization of origins of replication from beta-lactamase-producing plasmids of Neisseria gonorrhoeae.
… and several Toronto-type is 2,273 bp and the sequence spanning the deletion was identical irrespective of geographic or temporal origin. Thus, the Rio and Toronto-type plasmids are identical. The Nimes-type plasmid is proposed to be identical to the Africa-type but contains an IS 5 insertion …
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Molecular Characterization of Vancomycin-resistant Enterococci from Clinical and Surveillance Specimens
… regions of Tn1546 was performed. To clarify insertion sequence(IS), genomic DNA of isolates were amplified with a combination of Tn1546-derived primer and IS primer. Pulsed-field gel electrophoresis(PFGE) of SmaI-digested genomic DNA was performed using CHEF-DRII device. The banding patterns …
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Movement and Stability of the Staphylococcal Cassette Chromosome mec among Staphylococcus aureus
… site-specifically at the 5 ́ end of IS431 insertion sequence elements, but randomly at the other end. The explanation of the OS-MRSA isolates remains obscured, but likely relates to altered post-translational mechanisms or mutations in accessory proteins that have yet to be linked to a role …
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Molecular and phylogenetic analysis of a Bacillus thuringiensis genetic locus
… of $\delta$-endotoxins. N-terminal amino acid sequence analysis indicated that the larger proteins from each strain (90 and 72-kDa of 84-I and 70 and 65-kDa of 17A) were related to the Cry proteins of Bt ssp. $\textit{israelensis}$(Bti). Immunoblotting experiments confirmed that Cry10A-type …
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Phenotypic and genotypic characterization of salmonella isolates from chickens and humans in South Sudan.
… identified of which the majority belonged to the insertion sequence, miniature inverted repeat, unit transposon and integrative conjugative element. Human isolates showed twenty-four (80%) mobile genetic elements compared to chicken which had only six (20%) mobile genetic elements only. The …
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Genetic relatedness of Mycobacterium tuberculosis strains obtained from Kalafong Hospital in Pretoria using Spoligotyping and MIRU-VNTR typing
… of TB, its control and prevention. The insertion sequence 6110 restriction fragment length polymorphism (IS6110 RFLP) has been considered the „gold standard‟ in M. tuberculosis genotyping due to its high discriminatory power. However, due to limitations, such as the requirement of large …
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A streamlined Workflow for Transposon Mutagenesis in Corynebacterium glutamicum Based on Third-Generation Sequencing and its Application for Advanced Genetic Screens
… and utilization of the prophage- and insertion sequence(IS)-free *Corynebacterium glutamicum* platform strain CR101 for an efficient integration system, a transposon mutagenesis system based on the endogenous IS*Cg1* transposase and a streamlined workflow for transposon mutant library …
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Measurements and analyses of transposable element activity inside living cells
… selection methods, exact integration of long sequences (~6.5kbp) to any given target location, scar-less deletions, and gene fusion to native genes in situ.