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Showing 1 to 15 of 15 for “"initiation of DNA-replication"”.
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Investigating the Mechanism of Geminin Inactivation
DNA replication is regulated throughout the cell cycle to ensure that the entire genome is replicated once per cell cycle. Replication licensing is a key process required for the initiation of DNA replication. Replication licensing refers to the chromatin loading of an essential replication factor, …
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Mechanism and importance of Mcm2-7 double-hexamer formation during DNA replication initiation
… and accurately in each cell cycle. Thus, DNA replication is a highly-regulated multi-step process that ensures the genome is duplicated only once per cell cycle. In eukaryotic cells, initiation of DNA replication begins with loading of two heterohexameric Mcm2-7 helicases around origin DNA …
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Experimental studies of RctB- initiator of Vibrio cholerae chromosome II replication: genetic engineering, protein purification, and protein crystals
<p>Vibrio cholerae, a member of the Vibrionaceae family, is notorious as the causative agent of acute gastrointestinal disease known as cholera. Genetic information in members of the Vibrionaceae family is distributed between two chromosomes that are distinctively maintained. In V. cholerae, the …
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Creating Genetic Engineering Tools for Investigating Bacillus anthracis.
… and is endemic worldwide. Currently, the biology of <em>B. anthracis</em> is poorly understood. <em>B. anthracis</em> is one of many biological weapons used today. A -/- mutant strain of <em>B. anthracis</em> that lacks the pathogenic plasmids was created by serial culture at 42°C. Key DNA …
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Regulation of Ciz1 by Cyclin A-Cyclin Dependent Kinase 2 (CDK2) mediated phosphorylation
The progression of the cell cycle is a tightly regulated process, regulated by the activities of cyclin dependent kinases. Phosphorylation of target proteins regulates key transitions in the cell cycle contributing to accurate DNA replication and cell division. Here we evaluate the regulation of …
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Phosphorylation of the replicative helicase by the S-phase kinase, Dbf4-Cdc7, at origins of replication in Saccharomyces cerevisiae
In eukaryotic cells, events such as DNA replication and mitosis must be carefully coordinated in the cell cycle to ensure that the entire genome is duplicated before cells undergo cell division. In particular, initiation of DNA replication occurs in two temporally distinct steps, selection of sites …
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Mechanism of active substrate delivery by the AAA+ protease adaptor CIpS
… microtubule severing, membrane fusion, and initiation of DNA replication. Protein quality control in all organisms involves deployment of ATP-dependent proteases, consisting of hexameric AAA+ rings that unfold and translocate specific substrates into an associated peptidase barrel. Adaptor …
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Characterisation of non-essential genes required for survival under conditions of DNA stress
Genomic instability is a hallmark of cancer. Cancer progression depends on the development and amplification of mutations that alter the cellular response to threats to the genome. This can lead to DNA replication stress and the potential loss of genetic integrity of the newly formed cells. The …
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Analysis of cyclin-CDK mediated regulation of the G1/S transition in post quiescent murine fibroblasts
Eukaryotic cells have evolved a complex set of regulatory proteins to maintain genome stability by regulating DNA replication once and once per cell cycle. Despite intensive investigation, a precise understanding of the proteins that are phosphorylated during the initiation phase of DNA replication …
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Origins of non-coding Y RNA family and its role in DNA replication
Y RNAs are a family of non-coding RNAs that are required for chromosomal DNA replication initiation in vertebrates. Specifically, Y RNAs play an essential role in the establishment of new DNA replication forks during G1 to S phase transition. However, the mechanism by which Y RNAs contribute to …
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Regulation of cell fate asymmetry in Caulobacter crescentus by a complex of two component signaling proteins
Cellular asymmetry is critical to the generation of complexity in both metazoans and many microbes. However, several molecular mechanisms responsible for translating asymmetry into differential cell fates remain unknown. Caulobacter crescentus provides an excellent model to study this process …
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The Role of the Protein MTBP in DNA Replication
The initiation of eukaryotic DNA replication occurs at replication origins, licensed by loaded double hexamers of Mcm2-7 proteins. The action of two kinases, DDK and S-CDK, triggers the loading onto Mcm2-7 of Cdc45 and the GINS complex to form the replicative CMG helicase.<br/><br/>In yeast, the …
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Role of ORCA and ORC in chromatin organization and DNA replication
… at the origins and this is essential for the initiation of DNA replication. In human cells, ORC is a highly dynamic complex with many separate functions attributed to sub-complexes or individual subunits of ORC including heterochromatin organization, telomere and centromere function, …
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A Neural Network and Genome Language Model Based Approach to Understanding DNA Replication in Cancer
The rapid acceleration of technological innovation over the last two decades has catalysed an unprecedented surge in biological data generation, exemplified by the sequencing of the human genome with its ~6.4 billion base pairs, enough to fill nearly two million pages if printed out for a single …
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Interaktionen der Replikationsproteine der Maus
Zusammenfassung Die Initiation der DNA-Replikation in Eukaryonten ist ein hochkonservierter Prozeß, der in drei Stufen unterteilt werden kann. Im ersten Schritt bindet der ORC-Komplex an Replikationsorigins in chromosomaler DNA, wodurch die Assemblierung des präreplikativen Komplexes an den Origins …