Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"infectious clones"”.
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Molecular diagnostics, genetic diversity and generating infectious clones for cassava brown streak viruses
… strategies were carried out for generating infectious clones of CBSV; gateway cloning, in vivo and in vitro transcription methods, and amplification of the viral genome in three fragments. Although 3 overlapping CBSV fragments were successfully cloned, the presence of an unexpected mutation …
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Characterization of grapevine vein clearing virus expression strategy and development of caulimovirus infectious clones
… was used to evaluate the infectivity of an infectious clone of GVCV. Chapter 4 describes the construction of a terminally redundant clone of GVCV. The GVCV genome was assembled via three overlapping DNA fragments amplified from GVCV-infected tissues and the terminally redundant clone, …
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Development, Characterization, and Use of Molecular Tools to Study Immune-Driven Zika Virus Evolution
… due to the inherent toxicity of flavivirus infectious clones in bacteria. To mitigate the toxic nature of flavivirus clones, we developed several entirely in vitro workflows using a combination of rolling circle amplification (RCA) and replication cycle reaction (RCR). We demonstrated that …
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Usutu Virus: An Emerging Arbovirus Threat
… in the United States by: (1) developing infectious clones of recent European and African USUV isolates as tools for characterization and analysis of USUV and (2) assessing the transmission potential of several species of North American mosquitoes. In Aim 1, we show that the aforementioned …
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Spike Processing and Protease Usage Effects on SARS-CoV-2 Pathogenesis
… reverse genetics system, we generated several infectious clones to examine the various features of the CTS1: ΔQTQTN demonstrated the importance of loop length and glycosylation. PQQA established that the integrity of the FCS is necessary. Omicron CTS1 mutants YKH (H655Y, N679K, and P681H) and …
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Molecular Characterization of Viruses Infecting Greenhouse Vegetables in Ontario
… further characterized CGMMV and constructed an infectious CGMMV clone for the development of an attnuated virus for the control of CGMMV. We found that in the mimic greenhouse hydroponic production system, CGMMV is water-transmissible. We introduced various mutaitons into the CGMMV infectious …
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Construction and evaluation of infectious cDNA clones and analysis of packaging determinants of Soybean dwarf virus
… of soybean plants. In the present study, infectious clones of two dwarfing isolates of SbDV were constructed, and methods for Agrobacterium-mediated inoculation of plants were standardized and used to introduce them into fava bean, Nicotiana benthamiana, ‘Puget’ pea and red clover plants. …
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Characterization of Negeviruses and insect virome interactions during co-infection in cell culture
… exclusion will be tested through additional infectious clones of Negeviruses that have fluorescent reporter genes inserted into their genome. Analyzing the growth trends of different Negeviruses in these cell lines allow us to determine which Negevirus isolates can establish infection in the …
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Adaptation of Chikungunya virus to Aedes albopictus mosquitoes: The role of mutations in the E1 AND E2 glycoproteins
… by Ae. albopictus, I developed full-length infectious clone (i.c.) (pCHIKV-LR i.c.) and an i.c. that expressed enhanced green fluorescent protein (eGFP) from either a 3’ or 5’ additional sub-genomic promoters (pCHIKV-LR 3’GFP and pCHIKV-LR 5’GFP respectively) based on a CHIKV strain isolated …
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Investigation of the interplay of yellow fever virus structural protein epitopes and genetic diversity
… specific antibodies that bind E protein. Using infectious clones (i.c.) of both Asibi and 17D viruses, structural chimeras with swapped prME, E protein domain III (EDIII) and single site mutations at each of the residues that differ between WT and vaccine strain in structural proteins were …
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The Role of Interferon Stimlated Genes in Resistance and Immunity to Hepatitis C Virus Infection
… enhanced suppression of HCV genotype 1a and 2a infectious clones. Furthermore, a recombinant drug form of the OAS1 variant demonstrated broad antiviral activity, suggesting great promise for this protein as a therapeutic for HCV infection.
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Characterization of the molecular and biological determinants of viral dissemination from the mosquito midgut: Yellow feber virus in Aedes aegypti
… initial site of infection following uptake of an infectious bloodmeal- to infect the salivary glands and enter the saliva. The genetic determinants of viral dissemination from the midgut were characterized using the flavivirus yellow fever virus (YFV) in the Aedes aegypti mosquito as a model …
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Molecular characterization of tomato brown rugose fruit virus-host interactions and development of biocontrol strategies
… were cloned and sequenced, and full-length cDNA infectious clones were constructed. Defective mutation analyses revealed that ToBRFV movement protein (MP) and coat protein (CP) contribute to ToBRFV local accumulation and are critical for systemic infectivity. Two highly conserved CP residues, D89 …
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Reverse genetics system and fucntion of NSM protein of Rift Valley fever virus (family Bunyaviridae, genus phlebovirus)
… reverse genetics system to rescue infectious clones of RVFV MP-12 strain entirely from cDNA, the first for any phleboviruses. Using this system, RVFV carrying substitution mutations of the M gene preglycoprotein region (pre-Gn region), one lacking NSm protein expression, one lacking …
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Innate immune modulation and pathogenic basis for porcine reproductive and respiratory syndrome virus
… viruses (vL126A and vL135A) were generated using infectious clones. These mutant viruses retained the infectivity, and their phenotype was IFN suppression-negative and host mRNA nuclear retention-negative. Pigs infected with vL126A or vL135A exhibited milder severe clinical signs with lower viral …
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Reverse Genetics-based Approaches to Attenuate Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)
… PRRSV genes, we first established a DNA-launched infectious clone of the passage 14 PRRSV strain VR2385, pIR-VR2385-CA, and identified a spontaneous 435-bp deletion in the nsp2 gene. To characterize the biological and pathological significance of this nsp2 deletion, we restored deleted nsp2 …
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Molecular Characterization of Animal Strains of Hepatitis E Virus (HEV): Avian HEV and Swine HEV
… and genome organization, and to construct an infectious cDNA clone of avian HEV. The prevalence of swine HEV infections in US swine herds and the heterogeneity of swine HEV isolates from different geographic regions of the United States were determined. We found that 35% pigs and 54% swine …