Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"infectious cDNA clone"”.
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Molecular characterization of the major envelope protein of porcine reproductive and respiratory syndrome virus (PRRSV) and evaluation of its use for a diagnostic assay, vaccine development, and the examination of quasispecies evolution
… that inoculation of RNA transcripts of a PRRSV infectious cDNA clone directly into the lymph nodes and tonsils of pigs produces active PRRSV infection. Using this method, we also examined the quasispecies populations of PRRSV. Finally, we evaluated the ability of Salmonella choleraesuis to …
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Vesicular Stomatitis Virus as a Vector to Deliver Virus-Like Particles of Human Norovirus: A New Live Vectored Vaccine for Human Norovirus
… VP1 protein (rVSV-VP1) was recovered from an infectious cDNA clone of VSV. Expression of the capsid protein by VSV resulted in the formation of human NoV virus-like particles (VLPs) that are morphologically and antigenically identical to the native virions. Recombinant rVSV-VP1 was attenuated …
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Molecular characterization of tomato brown rugose fruit virus-host interactions and development of biocontrol strategies
… genomes of three ToBRFV Canadian isolates were cloned and sequenced, and full-length cDNA infectious clones were constructed. Defective mutation analyses revealed that ToBRFV movement protein (MP) and coat protein (CP) contribute to ToBRFV local accumulation and are critical for systemic …
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Molecular Characterization of Animal Strains of Hepatitis E Virus (HEV): Avian HEV and Swine HEV
… and genome organization, and to construct an infectious cDNA clone of avian HEV. The prevalence of swine HEV infections in US swine herds and the heterogeneity of swine HEV isolates from different geographic regions of the United States were determined. We found that 35% pigs and 54% swine …
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Understanding the Role of the Hypervariable Region in the Open Reading Frame 1 of the Hepatitis E virus in Viral Replication
… HVR region (aaΔ557-603) using the avian HEV infectious cDNA clone as the backbone. After confirming the viability of the complete HVR-deletion mutant in LMH cells, SPF chickens were intrahepatically inoculated with capped RNA transcripts generated from the mutant. None of the chickens …