Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"inducible system"”.
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Improvement and establishment of the tTA-dependent inducible system in the mouse brain
A genetic regulatory system employing the tTA that can induce individual gene expression reversibly in a temporal and spatial manner could permit a more precise analysis of gene functions on animal physiology and behaviour. For efficient expression of tTA in the mouse, the coding sequence of the …
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Evaluation of a reverse tetracycline inducible system in recombinant BCG to improve stability and immunogenicity
… the tetracycline dependent gene regulation system to down-regulate recombinant antigen expression in BCG during the expansion of seed stocks and up-regulate expression prior to or post vaccination.
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Design of a Tetracycline Operon Inducible System for the Control of Vaccinia Virus Replication: Implications for Vaccine Development
<p>The use of vaccinia virus (VACV) as a vaccine resulted in the eradication of smallpox in 1979. Characteristics that contribute to the effectiveness of VACV as a vaccine and viral vector include its ability to elicit strong, long-lived humoral and cell-mediated immune responses as a …
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Transcriptional Regulation of Shewanella oneidensis Using Native TMAO-Inducible Promoters in a Plasmid-Free System
… space, is crucial for EET. An ideal synthetic system utilizing S. oneidensis towards biotechnological applications would allow us to control mtr gene expression to dictate EET activity. Here I discuss a native S. oneidensis pathway that can be used to induce gene expression with trimethylamine …
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Role of Notch Signaling and GATA-3 in Establishing T-lineage Commitment and Survival in Developing Thymocytes
… circumvent this obstacle by establishing genetic systems, which allow to examine precise roles of Notch and GATA-3 in adult mice. Using RBPjind mice in which Notch responsiveness in all cells of hematopoietic lineage can be controlled with a Teton-inducible system, we demonstrate that Notch is …
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Intrinsic Specificity of Binding and Regulatory Function of Class II bHLH Transcription Factors
… sites remain poorly defined. Here, we utilize an inducible system to express these master regulatory factors in embryonic stem cells to characterize early events in bHLH factor binding and function in a common cellular context, removed from their role as endogenous master regulators of lineage …
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Telomere-Driven Tetraploidy and its Relevance to Cancer
… crisis in the absence of p53 and Rb. Using an inducible system to generate transient telomere dam- age, we show that telomere-driven tetraploidization enhances the tumorigenic transformation of mouse cells. Finally we showed that breast cancer cell lines with high chromosome numbers display …
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A SYNTHETIC TMRNA PLATFORM FOR ELUCIDATION OF BACTERIAL PROTEOME REMODELING UNDER STRESS
… a nearly ubiquitous native ribosome rescue system, transfer-messenger RNA (tmRNA), that rapidly resolves stalled translational complexes and tags the incomplete polypeptides for degradation. Characterization of these tmRNA-tagged polypeptides could reveal previously unknown aspects of the …
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Spatio-temporal analysis of florigenic signals in Arabidopsis thaliana, Sinapis alba and Brassica napus
… the floral transition, two independent induction systems in Arabidopsis were used: a photoextension system, whereby plants received fourteen additional hours of light, and a parallel dexamethasone-inducible system, which was centered on the induction of the known flowering gene CONSTANS (CO). …
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Outer Membrane Proteins Associated With Utilization of Chondroitin Sulfate by Bacteroides Thetaiotaomicron
… thetaiotaomicron employs a cell-associated inducible system to degrade chondroitin sulfate (CS), a mucopolysaccharide that may be a natural carbon source for this organism in the colon. The system that V. thetaiotaomicron uses to break down CS is not fully understood. Chondroitin lyase, an …
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Studies on cathepsin B of Eimeria tenella and pyroglutamyl peptidase of Leishmania major
… as a mature enzyme in the Pichia pastoris inducible system as a glycosylation protein, but the glycosylations did not apparently affect the enzyme’s activity. The biochemical characteristics of the recombinant enzyme were consistent with what has been reported in the literature for …
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The Role of Ribosomal Maturation Proteins WBSCR22 and EMG1 Methyltransferase Activity in Cancer
… proteins are knocked down through a stable shRNA-inducible system and rescued with a wild-type or a catalytically dead variant. In AML, inducible depletion of WBSCR22 and EMG1 through both shRNA knock-down and CRISPR-Cas9 targeting, causes cell cycle arrest which cannot be rescued by catalytically …
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Analysis of the functions of phosphoprotein phosphatase 1 catalytic subunits during cell division
… of using the auxin response factor (ARF)-auxin inducible degron (AiD) system for targeted proteolysis in hTERT-RPE-1 and found that this system resulted in leaky degradation of the target protein. I next tested the AiD2 system to study PP1 phosphatases and ultimately generated human colon tumour …
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An investigation of mutant p53 function.
… p53 hotspot mutants under the control of an inducible promoter. These cell lines were harnessed to investigate a range of wildtype and mutant p53 functions. The induced wild-type p53 protein is demonstrated to be transcriptionally and biologically active, and its function can be further …
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Mechanistic Investigation of Genotype-Phenotype Correlations in PIK3R1-Related Diseases
… Akt phosphorylation. Observations from a system with endogenous expression of mutant p85$\alpha$ Y657X supported the results obtained in the 3T3-L1 p85$\alpha$ overexpression models. The final part of this thesis focussed on a PIK3R1 exon skipping mutant (p85$\alpha$ $\Delta$Ex11) that …
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Callus Culture as a Prospective Biosensor: Examining Fusarium graminearum Infection and Exploring the Use of RUBY as a Pathogen-Inducible Reporter
… When transformed with a paired pathogen-inducible promoter (i.e., switches on in the presence of pathogens) and reporter (i.e., genes that trigger a phenotypic alteration when activated), this tissue could be a prospective candidate for a plant biosensor (i.e., phytosensor). We sought to …
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ZNF300 may influence the metastatic properties in pancreatic ductal adenocarcinoma
… ZNF300 using the p-RetroX-Tre3G Tet-ON inducible expression system. Following induction using doxycycline, both the expression and migratory ability were measured. The data in this study concludes that, contrary to preliminary data, the ZNF300 CpG island is not differentially methylated …
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COMPOSTI AD AZIONE ANTI-AGGREGANTE ESERCITANO UN EFFETTO BENEFICO SU MODELLI CELLULARI DI ATROFIA MUSCOLARE SPINALE BULBARE
… (112 polyGln repeats AR expressed through an inducible system) six compounds, AR antagonist or partial agonists analogs, defined as SARMs (Selective AR Modulator), known to prevent the N/C interaction but to permit the nuclear receptor transcriptional activity. The treatment of our cells with …
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Gain-of-Function Mouse Models to Investigate Biological Roles of Prmt6
… arginine methylation at the organismal level in inducible PRMT6 gain-of-function mouse models. To this purpose, I generated two different systems. One mouse model is based on an <em>ER*-Flag-PRMT6</em> fusion (under a beta-actin promoter), while the other model is based on a Cre-inducible system, …
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Effect of uptake pathway on non-viral gene delivery in vitro and in vivo
… utilizing a small hairpin RNA (shRNA) Tet-on system. Specifically, we have investigated the effects of clathrin-dependent endocytosis and caveolin-dependent endocytosis on the efficacy of PEI and PEI-derivative gene delivery in HeLa and MDA-MB-231 cell cultures and xenograft tumors. First, we …
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