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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"inducible promoter"”.
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Use of an Inducible Promoter to Characterize Type IV Pili Homologues in Clostridium perfringens
… pathogen, were lacking a tightlyregulated, inducible promoter system in their genetic toolbox. We constructed a lactose-inducible plasmid-based system utilizing the transcriptional regulator, BgaR. Using the <i>E. coli</i> reporter GusA, we characterized its induction in three different …
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The Utilization of the Hmg2 Inducible Promoter to Genetically Engineer Parasite Resistance in Tobacco
… toxins under the control of parasite-responsive promoters using tobacco (Nicotiana tabacum L. cv. Xanthi). For nematode resistance, an anti-feeding strategy was employed utilizing the tomato proteinase inhibitor I (PI-I) gene as a nematode specific toxin. Transgenic tobacco plants were generated …
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Characterization of a minimal abiotic stress-inducible promoter in the development of a binary vector for crop protection
… an orchestrated manner using customised stress-inducible promoters, which govern gene expression. Many studies have argued against constitutive promoters in GM-crop strategies, because while they allow for a continuous and stable expression pattern, this also often leads to unintended …
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Adding Upstream Sequence and a Downstream Reporter to the Bile Acid Inducible Promoter of <I>CLOSTRIDIUM scindens</I> VPI 12708
… pathway: of particular interest is the bile acid inducible operon, for which <i>Clostridium scindens </i>VPI 12708 serves as the model organism. There is a lack of knowledge on how this operon is regulated, so the goal of this project was to create a genetic construct consisting of upstream …
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The Development of Genetic Tools for Protein Characterization and Genetic Engineering in the Acetic Acid Bacterium Gluconobacter Oxydans
… system, a surface display system, and an inducible promoter system. The results of the construction of these three tools for use in G. oxydans and initial analyses of the effectiveness within the host are presented.
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Construction and Use of a Transposon for Identification of Essential Genes in Mycobacteria
… has an outward-facing anhydrotetracycline (ATc)-inducible promoter at each end. A mutant with TnQuoVadis inserted upstream of an essential gene may display normal growth in the presence of ATc, but exhibit no growth or severely diminished growth in the absence of ATc. TnQuoVadis was placed onto a …
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Transcriptional Regulation of Shewanella oneidensis Using Native TMAO-Inducible Promoters in a Plasmid-Free System
… I demonstrate its use in a strain with a TMAO-inducible promoter driving mtr gene expression. qRT-PCR data indicate that the inducible system increases mtr gene expression when the cells are exposed to TMAO. Additionally, TMAO induction of the synthetic system increased extracellular iron …
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The use of transgenic tobacco as a production and delivery system for a vaccine against hemorrhagic enteritis virus of turkeys
… gene was fused to either a constitutive plant promoter (35S) or a wound inducible promoter (hmg2) on plasmids adapted for Agrobacterium-mediated transformation. Approximately sixty transgenic plants of each construct were generated and determined to contain the HEV fiber gene based on …
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Characterization of a soybean BAG gene and its potential role in nematode resistance
… and soybean under the control of a nematode-inducible promoter significantly reduced nematode development demonstrating its potential use in engineering a novel form of nematode resistance in crop plants.
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The Expression and Characterization of Human Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Tobacco
… CFTR fused to the 35S double enhanced promoter could not be propagated in E. coli, suggesting that the CFTR product generated by "leaky" expression was detrimental to bacteria. Two strategies were undertaken to address the problem: 1) a plant intron was introduced into CFTR sequence and …
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Characterization of ftsA conditional lethal mutants shows that FtsA is required at early and late stages of cell division in Streptococcus pneumoniae
… expressed under the control of the PZn inducible promoter at the ectopic bga locus of the S. pneumoniae chromosome, fully complemented the Ts phenotype of two, A20TS and A21TS, mutants. Interestingly, the Ts phenotype of the A19TS mutant was only partially complemented by GFP-FtsAWT, …
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Tools and reference standards supporting the engineering and evolution of synthetic biological systems
… developing improved enzymes, transcriptional promoters, and fluorescent proteins have generated large libraries of variants and screened these libraries to find individual mutants that met desired performance specifications. In this thesis, I address some of the bottlenecks preventing the …
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Analysis of the aryl hydrocarbon receptor and a truncated form (AHR C[upper case symbol for greek Delta]Δ553) in cancer cells
… fused to pEGFP or a vector under a tetracycline-inducible promoter. Stable cell lines expressing pEGFP-AhR CΔ553 have been generated and confirmed to have nuclear localization. We were also interested in confirming endogenous localization patterns of AhR and Arnt to study the role of p23 in the …
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Development of CRISPR/Cas9-based gene drive biotechnology in S. cerevisiae
… illustrated that imperfect activation of the inducible promoter driving expression of the Cas9 nuclease or issues with multiplexing to artificial sequences may have resulted in a small percentage of resistant/inactive clones. The CRISPR/Cas9 system can also be used to regulate gene …
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The role of phospholipids in the growth and development of Streptomyces
… this gene was placed under the control of an inducible promoter, the strain developed poorly on agar, unless supplemented with the inducer of the promoter. We demonstrated that expression of SCO1389 and phospolipids became dependent on addition of the inducer to this strain. In order to …
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Accelerated Adaptation through Stimulated Copy Number Variation in Saccharomyces cerevisiae
… such CNV gene. CUP1 is transcribed from a copper inducible promoter and encodes a protein involved in copper detoxification. In this work I show that yeast can regulate their repeat levels of the CUP1 gene through a transcriptionally stimulated CNV mechanism, as a direct adaptation response to a …
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Development and validation of a CRISPR interference system for gene regulation in Campylobacter jejuni
… system was combined with C. jejuni-derived promoters of predetermined activities to develop a CRISPRi-based repression tool in C. jejuni strains M1Cam and 81-176. The tool was validated through successful repression of the arylsulphatase gene astA using a range of sgRNA target sequences …
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A cell-based sensor of fluid shear stress for microfluidics
… sensor requires identifying an appropriate inducible promoter to drive fluorescence expression upon FSS. To this end, the native mechanotransduction of a widely used and easy-to-culture cell line (NIH3T3s) was elucidated by culturing them in a microfluidic device and applying logarithmic FSS …
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Functional Characterization of the Streptococcus sanguinis com Regulon
… effect, while expression of the gene from an inducible promoter increased biofilm formation in the absence of CSP. Deletion of the comX gene resulted in biofilms with increased staining, cell death, and profoundly altered structure. Treatment with DNase I reduced biofilm formation in a …
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The Molecular Mechanisms of Antifungal Drug Resistance In Pathogenic Fungi
… ERG genes were overexpressed under a galactose inducible promoter. Nine of the 25 strains overexpressing the ERG genes showed severe growth defects. Furthermore, phenotypic changes in these strains were compared to wild-type under various stress agents. These agents affect several cellular …
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