Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 14 of 14 for “"inducible gene expression"”.
-
Inducible Gene Expression in Cell Cultures and in Microencapsulated Cells
… system provides a means to exogenously control gene expression in transformed mammalian cells in a temporal and spatial fashion. Such a promoter activation system consists of three components: The first of these is a steroidal inducer drug, mifepristone. This drug binds to the second component, …
-
Determining The Genotype-Phenotype Connection In Synthetic Inducible Gene Expression Systems
<p><strong>Introduction</strong> Gene expression is an important process whereby the genotype controls an individual cell’s phenotype. However, even genetically identical cells display a variety of phenotypes, which may be attributed to differences in their environment. Yet, even after controlling …
-
Creation of a Binary CuSO4 Inducible gene expression system in Arabidopsis thaliana and its application to the degradation of Lignin in planta
… approaches to chemically induce the overexpression of lignin degradation enzymes in planta within the model organism Arabidopsis thaliana and deliver them to the cell wall for controlled lignin degradation in a proof-of-concept study.
-
Investigating temperature signalling pathways in Arabidopsis thaliana using small molecules
… reprogramming of several hundreds of genes that promote stress tolerance. Despite extensive characterisation of the transcriptional responses to these stimuli, however, relatively little is known about the mechanisms by which temperature signals are perceived and transduced in plant …
-
Regulation and patterning of cell differentiation and pluripotency
… in applications such as tissue engineering and regenerative medicine. In this thesis, we explored the applications of controllable gene expression techniques in combination with engineering strategies in regulation and patterning of cell differentiation and pluripotency, by pursuing three related …
-
Integrated cellular systems
The generation of new three-dimensional (3D) matrices that enable integration of biomolecular components and whole cells into device architectures, without adversely altering their morphology or activity, continues to be an expanding and challenging field of research. This research is driven by the …
-
The role of SENSITIVE TO FREEZING6 in plant tolerance to stress
… often from sub-optimal agricultural areas. Genetic engineering can help meet these needs through the development of crops with greater stress tolerance. Identification of Arabidopsis mutants unable to tolerate stress is a powerful approach to investigate the molecular basis of stress …
-
Isolation of antimutator yeast strains: an exploration of DNA replication fidelity
… eex alleles were extragenic to the polymerase genes, suggesting that factors in addition to polymerase base selectivity, proofreading and MMR influence replication fidelity. Previous studies (Datta et al. 2000) showed that the mutator phenotype of proofreading-deficient Pol delta depends on …
-
Automated hydrodynamic trap for single cell analysis in free solution
… techniques have been developed to study inducible gene expression at the single cell level, albeit without the ability to control external stimuli with precise methods. Most are limited by long diffusive timescales to alternate environmental concentrations. In this work, we report a …
-
High-Throughput Discovery of Stimuli of Bacterial Two-Component Systems from the Human Gut Microbiome
… TCSs from the human microbiome, synthesized the genes, and transformed the library into E. coli. Each TCS controls the expression of a barcoded mRNA and a GFP reporter gene. Here, we develop a simple computational tool to infer TCS inputs based on nearby genes and predicted regulatory …
-
Mechanisms of dendritic peptide release
… correlated to loci of high vasopressin expression. This suggests active degradation of vesicles in dendrites. It is not known whether preferential release of peptides occurs along the profile of dendrites. Experiments were carried out using a temperature block to block exit of vesicles …
-
Creation of Versatile Cloning Platforms for Transgene Expression and Epigenome Editing and Their Application to Pancreatic Islet Biology
… previously reported that adenovirus-mediated overexpression of the transcription factor PDX1 is sufficient to induce β-cell replication, but underlying mechanisms remain to be resolved. Using statistical modeling, we identified the miR-17 family, a member of the miR17~92 miRNA cluster, as a …
-
Sucrose breakdown in the potato tuber
… zu untersuchen. Zunächst wurde ein induzierbares Genexpressions-System aus dem Schimmelpilz Aspergillus nidulans für die Untersuchung des Metabolismus von Kartoffelknollen optimiert. Es wurde herausgefunden, dass dieses sogenannte alc system schneller auf Acetaldehyd reagiert als auf Ethanol, und …
-
Characterization of Novel Type VI Effectors of Acidovorax citrulli and Their Applicability to Biological Control of Plant Diseases
… our T6SS-related studies, we successfully generate a series of salicylic acid (SA)-inducible vectors, functioning in A. citrulli, that can be used for inducible gene expression, protein purification and other applications. The core regulatory component that we employ, is a transcriptional …