Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 60 for “"induced Pluripotent Stem Cells (iPSCs)"”.
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Reprogramming to Pluripotency Using Small Molecule Compounds
<p>The generation of induced pluripotent stem cells (iPSCs) through the use of small molecule compounds has evolved as a potential cellular reprogramming strategy. Individually, specific small molecule compounds have previously been shown to replace reprogramming transcription factors or enhance …
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In vivo and in vitro approaches to characterising axon growth and metabolic alterations in Parkinson’s disease
… the time of disease onset, midbrain dopaminergic cells lose their synaptic connectivity before dying. The preposition that dopaminergic axons degenerate initially and predominantly in PD offers new critical opportunities for the development of neuroprotective and restorative therapies. However, we …
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Modelling Prenatal Hypoxia As A Risk Factor For Schizophrenia Vulnerability In Patient-Derived Cerebral Organoids
… has developed human cerebral organoids using induced pluripotent stem cells (iPSCs) derived from healthy control or SCZ patient cell lines to address these questions. This creates a platform that allows for the investigation into the pathophysiology of SCZ and hypoxia in tandem. Organoids were …
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Hematopoietic Stem Cell Threshold Sensing Controls Regulatory Pathways Facilitating Clinically Relevant Ex Vivo Expansion for Stem Cell Transplantation
<p>The ex vivo expansion of hematopoietic stem cells (HSCs) for transplantation has threefold importance: 1.) First, because of the rarity of stem cells there often isn’t a sufficient supply obtainable from common sources for larger children and adults. 2.) Secondly, patient morbidity and time to …
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Using single-cell RNA-seq to assess the effect of common genetic variants on gene expression during development
… development is partially overcome by advances in stem cell technologies. In particular, induced pluripotent stem cells (iPSCs) and cells derived therefrom represent a fantastic system to study development in vitro. In this thesis, I investigate the computational challenges of using single cell …
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Transcending the Cell Fate Stereotype: Cell Penetrating Peptide Technology helps Induce Pluripotency in Multiple Cell Types
<p>Induced pluripotent stem cells (iPSCs) are one of the most promising new technologies in the field of regenerative medicine. iPSCs can be generated directly from a patient’s own fibroblast cells then converted into any cell type, thus overcoming challenges associated with embryonic stem cells …
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Direct Reprogramming Of Fibroblasts Into Muscle Or Neural Lineages By Using Single Transcription Factor With Or Without Myod Transactivation Domain
The generation of induced pluripotent stem cells (iPSCs) from somatic cells has opened new doors for regenerative medicine by overcoming the ethical concerns surrounding embryonic stem (ES) cell research. However, iPSC technology presents several safety concerns, such as the potential risk of tumor …
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Investigating Clozapine-Induced Myocarditis in Stem Cell-Derived Cardiomyocytes from Treatment-Resistant Schizophrenia Patients: A Pharmacoepigenomic Approach
… may predispose individuals to clozapine-induced myocarditis, aiming to uncover biomarkers that can predict risk of this adverse effect and thus improve clinical outcomes. Utilizing a novel in vitro model, I used induced pluripotent stem cells (iPSCs) derived from patients with …
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Role of nuclear-FGFR1 in Schizophrenia and Ontogenic Genome Programming – A Computational Study
… genome at proneuronal stage – best progenitor cells commit to neural cells. In particular, the mRNA transcriptome nuclear FGFR1 (nFGFR1) was found to be dysregulated. Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) derived cerebral organoid from four control and …
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Correction of Cystic Fibrosis-Specific Induced Pluripotent Stem Cells
… the ability to genetically modify the resulting induced pluripotent stem cells (iPSCs) have provided an alternative to conventional gene therapies thus far attempted for CF. Here we describe the <em>in vitro</em> generation of iPSCs starting from fibroblast cells obtained from a CF patient. These …
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Investigating Induced Pluripotent Stem Cells for Tissue Engineering and Hepatotoxicity Applications
Induced pluripotent stem cells (iPSCs) can be differentiated into multiple cell types in the body while maintaining proliferative capabilities. The generation of human iPSC-derived hepatocytes (iPSC-Heps) has resulted in a new source for hepatic cells. The current available options for human …
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Engineering rFVIIa-loaded platelets; a novel approach to treating acute bleeding
… accidents. Platelets are small anucleate cells that exist in very large quantities in our blood and, along with cross-linked fibrin from the coagulation cascade, are involved in forming a haemostatic plug upon exposure to damaged endothelium in a wound. They are metabolically active and …
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Highly efficient Cas9 mediated transcriptional programming and delivery via Adeno-Associated Virus
… we stimulate neuronal differentiation of induced pluripotent stem cells (iPSCs), validating the biologically relevant levels of activation attained by this tool. Beyond applications to cellular programming, Cas9 transcriptional and epigenetic activators hold tremendous promise for in vivo …
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EPIGENETIC AND FUNCTIONAL ASSESSMENT OF ENHANCEROPATHIES ACROSS HUMAN MODELS: FOCUS ON GABRIELE-DE VRIES SYNDROME
… reprogrammed patient-derived and healthy somatic cells into induced-pluripotent stem cells (iPSCs) and observed, already at the pluripotent stage, a major transcriptional dysregulation. Moreover, since YY1-mutated patients exhibit ID features, we differentiated our cohort of iPSCs into cortical …
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Using induced pluripotent stem cells to model glial-neuronal interactions in TDP-43 proteinopathies
… model these diseases, based on patient-derived induced pluripotent stem cells (iPSCs). This study presents the optimization of an iPSC-based platform to study the consequences of TDP-43 M337V mutation in human functional populations of MNs and astrocytes in isolation as well as in co-culture. To …
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Promoting multi-omic rejuvenation in human and mouse somatic cells by transient reprogramming
… is the process of converting somatic cells, such as fibroblasts, into induced pluripotent stem cells (iPSCs). Amazingly, this process reverses age associated alterations in the epigenome and resets epigenetic clocks to 0 years old. Complete iPSC reprogramming has the major caveat of …
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Cellular And Mechano-Active Material Approaches To Improve Disc Repair After Herniation
… disc function. Furthermore, adult disc cells are sparse and have decreased biosynthetic abilities. Disc herniations, resulting from annular tears, enable the extrusion of NP tissue which can result in spinal nerve compression and consequent pain. The gold standard for the surgical …
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Infusing Factor Viii-Expressing Platelets Or Megakaryocytes As A Novel Therapeutic Strategy For Hemophilia A
… such a product would be generated beginning with induced-pluripotent stem cells (iPSCs). iPSC-derived megakaryocytes, termed iMks, that are modified to express pFVIII may then be used to improved hemostasis in problematic inhibitor patients with hemophilia A. As proof-of-principle, we demonstrate …
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Using molecular QTLs to identify cell types and causal variants for complex traits
… now be modeled in vitro by differentiating human induced pluripotent stem cells (iPSCs) into specific cell types, including inaccessible cell types such as those of the brain. In this thesis, I explore a range of approaches for using QTLs to identify causal variants and to link these with …
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Lineage-Specific Phenotypic Abnormalities in iPSC-Derived Smooth Muscle Cells from Type-I Loeys-Dietz Syndrome
… develop at the aortic root, where smooth muscle cells (SMCs) from two distinct embryonic origins – the lateral mesoderm (LM) and neural crest (NC) – are intermingled. SMCs are hypothesised to be the key cell type contributing to TAA development. However, only LM-SMCs showed abnormal …
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