Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"in-gel digestion"”.
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Optimized GeLC-MS/MS for Bottom-Up Proteomics
Despite tremendous advances in mass spectrometry instrumentation and mass spectrometry-based methodologies, global protein profiling of organellar, cellular, tissue and body fluid proteomes in different organisms remains a challenging task due to the complexity of the samples and the wide dynamic …
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Characterization of Protein Complexes and Protein Interaction Networks by Mass Spectrometry
… an experimental proteomics approach for deciphering protein complexes and protein interaction networks in the budding and fission yeasts. Key steps of the employed analytical routine, including the purification of complexes and mass spectrometric identification of their subunits, were …
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Mechanism of metastasis of triple negative breast cancer
Triple Negative Breast Cancer (TNBC) is increasingly recognised as a serious, worldwide public health concern. TNBC has the ability to recur after treatment and appears to be greatest in the first few years. The phenotypical changes of the TNBC metastasis represent a unique that heterogeneous …
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Method development and proteomic profiling of boar seminal plasma using mass spectrometry-based workflows
Artificial insemination is an important technology in the swine industry; it is of great significance in the preservation and improvement of elite breeds. Conventional methods used in assessing semen lack precision, and the use of low fertile boars in breeding systems is prominent. Using mass …
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Cloning, Expression and Purification of the Different Human Haptoglobin Chains and Initial Characterization by Mass Spectrometry
Haptoglobin (Hp) is an acute phase glycoprotein that exists in all vertebrates and can be found in the majority of body fluids. It is phenotypically variant in humans and presents in two allelic forms: Hp1 and Hp2. The two forms vary by the identity of their light chains: L1 or L2 for Hp1 and Hp2, …
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Interactions of food proteins with plant phenolics – modulation of structural, techno- and bio-functional properties of proteins
… the largest group of secondary metabolites in plant foods. The phenolic compounds, e.g. chlorogenic acid (CQA), are susceptible to oxidation by enzymes specially, polyphenol oxidase (PPO) and at alkaline conditions. Both enzymatic and non-enzymatic oxidations occur in the presence of oxygen …
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Identification of interacting partners of mammalian target of rapamycin complex 1 (mTORC1) assembly in human lymphocytes
The mammalian target of rapamycin (mTOR) is an intracellular protein kinase that plays a key role in the control of cell growth, differentiation, cell survival, and cell proliferation. In humans, mTOR deregulation is implicated in parasitic, fungal, bacterial and viral infections, immune disorders, …