Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"in vivo assay"”.
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Water Status, High Temperature, and Genotype Effects on Some Processes Which Influence Maize Seed Set (Zea Mays L., Low Water Potential, Pollen Viability, in Vivo Assay, Stress)
Drought and high temperatures during pollination and fertilization greatly reduce kernel number in maize (Zea mays L.). Studies were conducted to determine the response of ear receptivity (the ability of the ear to set seed if the silks are pollinated), pollen viability, and pollen production to …
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"The use of retinoids and an in vivo ""affinophoresis"" assay to analyze the cellular basis of positional memory in regenerating axolotl limbs"
… to the amputation plane, and have been shown to inherit an expressed memory of their position of origin, termed positional value, by developing autonomously when grafted away from their normal limb site, and by inducing intercalary regeneration from more proximal limb stumps. Recently, retinoids …
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Developing Detection Methods for Nitroxyl
… of nitroxyl (HNO) is essential to understanding its bioactivity, metabolism, and relation to health and disease. However, detection and quantitation of HNO has been complicated by the rapid self-consumption of HNO through irreversible dimerization, poor selectivity of trapping agents against …
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Evaluation of Seizure Threshold as an Early Behavioral Marker of Disease Progression in the Mouse Model of Mucopolysaccharidosis IIIA
… a lysosomal storage disease caused by a mutation in the gene that codes for the enzyme heparan sulfamidase. The decreased enzyme activity of heparan sulfamidase results in the accumulation of heparan sulfate (HS). HS accumulation in the brain causes severe central nervous system (CNS) …
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Screening and evaluation of bacteria for the biological control of Botrytis cinerea on Vitis vinifera
Agriculture plays a significant role in food security and the economic sector. However, the agriculture industry is threatened by plant pathogenic microorganisms, with fungi accounting for 42% of plant diseases. Botrytis cinerea is the most notable of all plant pathogenic fungi. B. cinerea is …
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Cellular Functions of the Squash Leaf Curl Geminivirus Movement Proteins BL1 and BR1
… encode what are typically called movement proteins (MPs). These are defined as proteins dispensable for viral replication and encapsidation, but essential for cell to cell movement of the virus to the phloem and eventually leading to systemic infection of the plant. Unlike most plant viruses …
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Characterization of P-glycoprotein expression as a multixenobiotic resistance mechanism in fish
Transmembrane P-glycoproteins (P-gps) are responsible for multidrug resistance (MDR) phenotypes in tumor cell lines. P-glycoproteins function as energy dependent efflux flippases that prevent the cellular accumulation of a wide variety of compounds. We characterized P-gp expression in populations …
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Biochemical characterization of the first step in ether lipid biosynthesis: acylation of dihydroxyacetone phosphate
… for cellular growth and proliferation. It is an indispensable pathway in many living organisms. In mammals four GPAT isoforms are known to catalyze the first step in glycerolipid biosynthesis which involves acylation of glycerol-3-phosphate to lysophosphatidic acid. GPATs 1 and 2 localize to the …
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Factors affecting common lambsquarters' tolerance to imazethapyr
… and laboratory experiments were conducted to examine the tolerance mechanisms of common lambsquarters (Chenopodium album) to imazethapyr (2- (4,5-dihydro-4-methyl-(1-methylethyl)-5-oxo-1H-imidazol-2-yl) -5-ethyl-3-pyridinecarboxylic acid, active ingredient of Pursuit$\sp\circler$ herbicide) by …
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The S. cerevisiae calponin homologue SCP1 regulates stability and organization of the actin cytoskeleton
Calponins and transgelins are members of a conserved family of actin-associated proteins widely expressed from yeast to humans. While a role for calponin in muscle cells has been described, the biochemical activities and in vivo functions of non-muscle calponins and transgelins are largely unknown. …
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Structural determinants of murine leukemia virus (MLV) reverse transcriptase (RT) important for fidelity and drug resistance in vivo
… (RTs) is a major contributor to variation in retroviral populations generating mutants that exhibit altered host tropisms, resistance to antiviral drugs, and the ability to escape the host's immune system. To identify structural elements of murine leukemia virus (MLV) RT important for …
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Structural determinants of murine leukemia virus reverse transcriptase that are important for template switching, fidelity, and drug resistance
… high mutation rates. High mutation and recombination rates increase variation within a viral population and result in production of drug-resistant mutants and/or mutants that can escape a host immune response. Mutations are introduced into the viral genome by error-prone reverse transcriptase …
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Design, synthesis, and biological evaluation of sigma receptors (σRS) ligands as potential pharmacotherapy for cancer and drug addiction
Sigma receptors are a well-defined unique class of receptors and are highly expressed in the central nervous system and also widely distributed in peripheral organs and tissues. There are two subtypes of sigma receptors: sigma-1 and sigma-2. These receptors are thought to be associated with …
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Biochemical And Structural Characterization Of The Core Subunits Of Gpi Transamidase
… Doctor of Philosophy</p> <p>Glycosylphosphatidylinositol transamidase (GPI-T) is a complicated, membrane-bound, multi-subunit enzyme that catalyzes an essential post-translational modification. This enzyme attaches GPI anchors to the C-termini of various proteins that contain a proper GPI-T …
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Mechanisms Of Presynaptic CaV2 Calcium Channel Localization In Caenorhabditis Elegans
<p>Neurotransmitter release at nerve terminals is a fundamental aspect of communication in the nervous system. Voltage changes in the presynaptic membrane are sensed by presynaptic voltage-gated calcium channels that mediate calcium influx at the nerve terminals to execute exocytosis of various …
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Characterisation of XvPrx2 : a type II peroxiredoxin isolated from the resurrection plant Xerophyta viscosa (Baker)
… stresses is necessary for genetic engineering approaches to improve crop performance. A unique feature of resurrection plants, such as Xerophyta viscosa, is their ability to cope with severe water loss of greater than 90%. A full-length cDNA library was synthesised from a cold stressed …
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The essentiality of DivIVAEf oligomerization for proper cell division in enterococcus faecalis and interaction with a novel cell division protein
DivIVA is a Gram-positive cell division protein involved in chromosome segregation, midcell placement of the cell division machinery, complete septum closure, and polar growth and morphogenesis. Although well conserved across various Gram-positive species, DivIVA is believed to be relatively …
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A novel in vivo tumor oxygen profiling assay: Combining functional and molecular imaging with multivariate mathematical modeling
… to develop and test a novel high spatio-temporal in vivo assay to quantify tumor oxygenation and hypoxia. The assay implements a biophysical model of oxygen transport to fuse parameters acquired from in vivo functional and molecular imaging modalities. ^ Introduction: Tumor hypoxia plays an …
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The Identification and Development of Small Molecule Inhibitors of Amyloid β Aggregation
Amyloid $\beta$ (1-42) (A$\beta$42) is a seminal neuropathic agent in Alzheimer’s disease (AD), a multifaceted neurodegenerative disorder for which no preventative measures or disease modifying therapies currently exist. Aggregation of this peptide plays a key role in the synaptic dysfunction and …
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Apobec1-Mediated RNA Editing in Monocytes Contributes to Genetic Heterogeneity and Modulates Monocyte Activity and Development
… modifications such as alternative splicing have been shown to add to the complexity needed to compensate for the relatively low number of genes found in higher organisms. Many other modifications recently found in mRNA, which cannot be deduced from what is coded in the genome, such a …
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