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Showing 1 to 20 of 54 for “"in vitro transcription"”.
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In vitro transcription studies of adenovirus
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1983.
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Aspects of in Vivo and in Vitro Transcription in Yeast
… were employed to study the mechanisms of transcription by yeast RNA polymerase II and the yeast mitochondrial RNA polymerase both in vivo (in plasmid containing cells) and in vitro (in cell-free protein extracts). A complete in vivo transcription map of the yeast centromere plasmid YCp19 …
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An in vitro transcription termination system to analyze spinach chloroplast promoters
To determine whether chloroplast RNA polymerase would accurately terminate transcription in vitro, the 3$\sp\prime$ end of the rbcL gene as well as various factor-independent transcription terminators from E. coli were fused to the spinach chloroplast rbcL promoter. Transcription of the rbcL …
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Localization, Enrichment, and in Vitro Transcription of the Ribosomal Rnagenes in Escherichia Coli
Made available in DSpace on 2014-12-11T18:16:51Z (GMT). No. of bitstreams: 1 7219797.pdf: 4385704 bytes, checksum: 5eadf6ee3efaedcac8a60213e328231f (MD5) Previous issue date: 1972
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Purification of Global Regulator, Spx, and RNA Polymerase from Staphylococcus aureus for Use in In Vitro Transcription of Redox Genes
Spx is a global regulator discovered in Bacillus subtilis to suppress basal growth and development processes and activate transcription of genes involved in thiol homeostasis when a cell encounters oxidative stress. Its activity relies on reversible thiol-disulfide bond formation and binding RNA …
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Structural and Biochemical Studies of the Innate Immune Pathway
Investigating the biochemical properties of the IRAK-4 DD led to a series of molecular recognition experiments with MyD88. In vitro transcription and translation pull-down experiments performed with IRAK-4 DD and MyD88 suggest that the ID of MyD88 might mediate this interaction.
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Essential features of a PrfA-dependent : promoter of Listeria monocytogenes
The gram-positive, facultative intracellular pathogen Listeria monocytogenes is the causal agent of listeriosis. Most of well-known virulence genes are controlled by PrfA that belongs to the Crp-Fnr family of transcriptional activators. A PrfA-mediated transcription initiating at a virulence gene …
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Interactions Among Steroid and Xenobiotic Response Cascades in the Regulation of CYP6B1
In support of this idea, treatment with the synthetic aryl hydrocarbon benzo[alpha]pyrene or the plant signaling molecule salicylic acid in combination with xanthotoxin resulted in in vitro transcription that was similar to treatment with either chemical alone, suggesting that the response cascades …
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Επίδρση της Ν.Ν.- Διμεθυλο - Νιτρωδοαμίνη στο μεταβολισμό του RNA σε ΜΥΕΣ C57BL
ADMINISTRATION OF A SINGLE DOSE OF THE HEPATOCARCINOGEN DIMETHYL-N- NITROSAMINETO MALE C57B1 MICE CAUSES AN INHIBITION OF HN-RNA SYNTHESIS. THE EXTENT OF THE INHIBITION DEPENDS ON THE DOSE ADIMINISTERED: THUS 4 HOURS AFTER THE ADMINISTRATION OF A DOSE OF 40MG/KG DIMETHYLNITROSAMINE, THE INHIBITION …
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Construction of a COL11A1 Transgene Vector
… Cartilage disorders affect millions of people in the United States alone, with effects ranging from poor skeletal development and joint pain to shortened lifespan and perinatal lethality. Many of these disorders have their root in defects of collagen, type XI collagen being among the most …
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The molecular mechanisms of transcription termination and pausing in the Escherichia coli threonine operon regulatory region
To study the mechanism of transcription termination at a rho-independent terminator, homoduplex and heteroduplex templates that contained mutations in the G + C-rich region of dyad symmetry in the Escherichia coli thr operon attenuator were constructed. Assays of these templates in an in vitro …
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The Role of Histone H2B Ubiquitylation and its Related Factors in Transcriptional Regulation in Mammalian Cells
… and phosphorylation play important roles in transcriptional regulation throughout eukaryotes, and recent studies in yeast also have implicated H2B ubiquitylation in the transcription of specific genes. However, a systematic study of H2B ubiquitylation in mammalian cells has been hindered …
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Cell-free expression systems for the construction of artificial cells
… systems are widely used to synthesize proteins for subsequent further characterization, to manufacture potentially useful commercial end products, and to construct cellular mimics in the laboratory. The first part of the thesis explores the feasibility of preparing two of the commercially …
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CHARACTERIZATION OF THE RNA BINDING MODE OF A PLATINUM-ACRIDINE AGENT
Pt-ACRAMTU (ACRAMTU = 1-[2-(acridin-9-ylamino)ethyl]-1,3-dimethylthiourea) is a DNA-targeted anticancer agent that shows activity in a broad range of solid tumor cell lines. Unlike the classical platinum-based cross-linking drugs, this agent produces its cytotoxic effect by two synergistic DNA …
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Site-Directed Mutagenesis of Southern Bean Mosaic Virus (Cowpea Strain): in Search of a Viral Protease
The second and largest open reading frame within the southern bean mosaic virus (SBMV) genome encodes a 105 kDa polyprotein. Following translation, the polyprotein is cleaved to liberate various proteins necessary for SBMV replication. The elements within polyproteins of the picornavirus …
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Mechanistic studies on transcription activation via DNA looping in a prokaryotic promoter-enhancer system
In this thesis, transcription activation was studied in a prokaryotic promoter-enhancer system. It comprises the E. coli RNA polymerase, which is associated with the alternative sigma factor sigma54 (RNAP-sigma54), and the transcription activator protein NtrC (nitrogen regulatory protein C), which …
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Genetic and Biochemical Properties of Arabidopsis RNA Polymerases IV and V
… RNA Polymerase II: Pol II). The largest subunits in Pol IV and Pol V, NRPD1 and NRPE1 respectively, share a second largest subunit, NRPD2/NRPE2. The evolutionarily conserved Metal A and Metal B binding sites are required for Pol IV and V in vivo function fitting the prediction that these are …
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Site Directed Mutagenesis of a Putative Protease Cleavage Site Within the 105 Kilodation Protein of Southern Bean Mosaic Virus
There are four open reading frames (ORF) in three positive-sense translational phases of Southern Bean Mosaic Virus (SBMV). ORF2 codes for a 105 KDa polyprotein which is proposed to be autocatalytically cleaved into smaller functional proteins. Amino acid sequence homology between the SBMV and …
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Molecular characterization of the cell division gene ftsZ in Neisseria gonorrhoeae.
Cell division is an essential process in all bacteria, although in some organisms like Neisseria gonorrhoeae relatively little is known about cell division. The process has been well characterized in Escherichia coli, with the identification and characterization of many cell division genes. The …
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