Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"in vitro expansion"”.
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In vitro Expansion von hämatopoetischen Stamm- und Progenitorzellen durch Komponenten der Stammzellnische
A successful in vitro expansion of hematopoietic stem- and progenitor cells (HPC) is important for reliable cord blood stem cell transplantations with low cell numbers. For in vitro cultivation of HPC, there are no safe protocols existing which provide the ability to control self-renewal and …
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Optimierung der Proliferation und Differenzierung humaner Präadipozyten durch den Einsatz von humanem Serum
… of soft tissue defects is a challenge in plastic surgery and there is clinical need for adequate solutions. A promising solution is adipose tissue engineering with preadipocytes. These adipogenic precursor cells have the ability to proliferate and differentiate into adipose tissue after …
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Primary culture of human limbal-corneal epithelial cells under different conditions
… or loss of the LSC population leads to the painful and blinding condition of LSC deficiency (LSCD). In vitro expansion of LSCs is an increasingly well recognized treatment modality for LSCD. The purpose of this study was to analyze the outgrowths from human cadaveric limbal explants cultured …
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Toward understanding and mitigating heterogeneity in bone marrow stromal cell cultures for improved therapeutic efficacy
… mesenchymal stem cells (MSCs), have been used in over 600 clinical trials for indications ranging anywhere from autism to liver cirrhosis to diabetes. They have cited enthusiasm in the cell therapy community not only for their demonstrated differentiation potential toward several lineages, but …
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Role of Wnt signaling in proliferation of human bone marrow-derived mesenchymal stem cells
… of adult stem cells for the therapeutic use in regenerative medicine. These cells are usually culture expanded prior to their application. However, their proliferation and differentiation potential were gradually decreased during in vitro expansion. Wingless-related MMTV integration site …
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Sustained Depolarization of the Resting Membrane Potential Regulates Muscle Progenitor Cell Growth and Maintains Stem Cell Identity in vitro
It is important to maintain the myogenic properties of muscle progenitor cells (MPCs) during in vitro expansion for stem cell therapies and tissue engineering applications. Controlling cell fate for biomedical interventions will require insight on all aspects that influence cellular properties. The …
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An in Vitro Microenvironment for Nucleus Pulposus Regeneration
Intervertebral disc degeneration begins early in life, increases with age, and can ultimately result in disc failure. At the center of the disc, the nucleus pulposus (NP) resides in a hypoxic environment where degeneration is thought to begin. Initially, nucleus pulposus cells (NPCs) display …
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Investigation of a pancreatic derived progenitor cell population for use in regenerative medicine
Diabetes mellitus remains a significant cause of morbidity and mortality despite best current treatment with insulin replacement therapy. Pancreatic stem cells or progenitor cells have been proposed as an attractive therapy either as a source of cells for in vitro expansion and differentiation to …
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Analyses of hematopoiesis and lineage commitment from ES and iPS cells
… zu verschiedenen Arten hämatopoietischer Zellen in vitro wurde optimiert und standardisiert, um eine zeitlich definierte, quantitativ auswertbare zelluläre Entwicklung zu verfolgen.Diese standardisierten in vitro-Zellkultursysteme ermöglichten eine quantitative Analyse der Kapazitäten …
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INFECTION- AND VACCINE-INDUCED IMMUNE RESPONSES TO SARS-COV-2 AND OTHER HUMAN CORONAVIRUSES
… syndrome coronavirus 2 (SARS-CoV-2) emerged in December 2019 and spread across the world causing the COVID-19 pandemic. Unprecedented efforts by scientists across the world saw the development and approval of different mRNA and adenovirus-vectored vaccines targeting the spike protein of the …
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Genetic programming of human iPSC-derived macrophages provides a tool to study the Erythroblastic Island niche in vitro
Macrophages have been attracting much attention as they are present in many tissues and organs, and are involved in homeostatic tissue function and disease. Studies involving human macrophages have been hampered due to the ethical constraints and technical difficulties related to their …