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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"in vitro evolution"”.
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In vitro evolution of ribozymes for self-synthesis
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In vitro-Evolution und Analyse der biophysikalischen Grundlagen der Proteinstabilität
In der vorliegenden Arbeit wurde Proside, ein Selektionssystem für stabilisierte Proteine, weiterentwickelt und dafür genutzt, die molekularen Ursachen erhöhter Proteinstabilität zu analysieren. Für diese in vitro-Evolutionsexperimente diente das Kälteschockprotein Bs-CspB aus Bacillus subtilis als …
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Stabilisierung von Proteinen durch in vitro-Evolution - Selektion und biophysikalische Charakterisierung
Die vorliegende Arbeit hatte das Ziel, große Proteine bzw. Enzyme durch Proside zu stabilisieren und die selektierten Varianten biophysikalisch zu charakterisieren. Proside ist eine auf dem phage display basierende in vitro-Evolutions-Methode zur Selektion stabilisierter Proteinvarianten, wobei …
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In Vitro Evolution of Antibody Affinity using Libraries with Insertions and Deletions
In Nature, antibodies are capable of recognizing a huge variety of different molecular structures on the surface of antigens. The primary factor that defines the structural diversity of the antibody antigen combining site is the length variation of the complementarity determining region (CDR) …
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Thermodynamische Stabilisierung von Proteinen durch in vitro Evolution und biophysikalische Analyse ihrer molekularen Grundlagen
Proteine sind zentrale Bausteine des Lebens und ihre dreidimensionale Struktur ist für die Funktion von entscheidender Bedeutung. Deswegen ist das Verständnis der konformationellen Stabilität von Proteinen von großem Interesse. In der vorliegenden Arbeit wurde das Selektionssystem Proside zur …
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Molecular engineering of new protein labeling methodology based on rational design and in vitro evolution
Site-specific labeling using E coli biotin ligase (BirA) and its 15-amino acid "acceptor peptide" (AP) has been applied to study the function of various cellular proteins. In order to extend the capabilities of biotin ligase-based labeling, we engineered key elements of the labeling platform. First …
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Divergent Evolution of Enzymatic Activity: Functional Assignment and in Vitro Evolution in the MLE Subgroup of the Enolase Superfamily
… generalized strategies for the divergent evolution of new enzymatic function, particularly within the context of enzymes containing the ubiquitous (beta/alpha)8-barrel fold. We have used the paradigm of the enolase superfamily and genomic context to functionally assign unknown proteins as …
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RNA-Mediated Synthesis of Particles from Organometallic Palladium and Platinum Precursors
… hexagonal, cubic and spherical palladium and platinum containing particles. In vitro selection techniques were used to evolve an initial library of ˜ 3 x 10¹³ unique RNA sequences through six to ten cycles of selection to yield several active sequence families. The particle growth occurred in …
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Modifikuotų nukleotidų taikymas plataus masto nukleorūgščių analizei /
… base-modified nucleic acids are widely used in various fields. The aim of this dissertation was was to investigate the properties and utility of oligonucleotide-tethered 2′,3′-dideoxyribonucleoside 5′-triphosphates (OTDDNs), with emphasis on labeling of DNA and cDNA molecules for …
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The utility of modified nucleotides for high-throughput nucleic acid analysis /
… base-modified nucleic acids are widely used in various fields. The aim of this dissertation was was to investigate the properties and utility of oligonucleotide-tethered 2′,3′-dideoxyribonucleoside 5′-triphosphates (OTDDNs), with emphasis on labeling of DNA and cDNA molecules for …
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Site-specific labeling of cellular proteins with unnatural substrates of biotin ligases
E. coli biotin ligase (BirA) catalyzes the site-specific ligation of biotin to the lysine within its 15-amino acid peptide substrate (AP). We harnessed the high peptide substrate specificity of BirA to develop a general methodology for site-specific protein labeling with biophysical probes. We …
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[RNA polymerase ribozymes]
The RNA World is a hypothetical ancient evolutionary era during which RNA was both genome and catalyst. During that time, RNA was the only kind of enzyme yet in existence, and one of its chief duties was the replication of RNA. This scenario presupposes that among all possible RNA sequences, there …
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Ice as a medium for RNA-catalysed RNA synthesis and evolution
A critical event in the origin of life is thought to have been the emergence of a molecule capable of self-replication and evolution. According to the RNA World hypothesis, this could have been an RNA polymerase ribozyme capable of generating copies of itself from simple nucleotide precursors. In …
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Protein Engineering via in Vitro Coevolution
The creation of novel protein functions represents a significant challenge in protein engineering. To address this challenge, I have developed a simple and efficient method---in vitro coevolution---for the creation of novel protein functions in an existing protein scaffold. The in vitro coevolution …
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Towards Enzymatic Synthesis of Uncharged Xeno Nucleic Acids
A genetic material is a defining requirement for life. The many practical uses of DNA and RNA have also made these molecules integral tools for human manipulation of biological systems. However, high susceptibility to nuclease degradation and limited chemical diversity constrain their use in …
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The influence of genetic background on drug resistance in the malaria parasite Plasmodium falciparum
… resistance to modern antimalarials emerging and spreading in certain parasite populations, understanding the mechanisms for acquiring multidrug resistance will be key for predicting which genes may become important for clinical resistance in the future. Transporter proteins have key roles …
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HCV-Diagnostik mittels genotypspezifischer Antikörper
… and reliable diagnostic system to discriminate between HCV genotypes 1b, 2 and 3. The envelope protein E2 sequence of genotype 1b was isolated from patients blood whereas consenus sequences had to be deduced from all available genotype-2 and -3 sequences. Sequences were derived from all …
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Mechanisms of cell adhesion regulation by herpes simplex virus
… human pathogen that establishes a life-long infection. HSV-1 promotes its replication and spread by expressing multi-functional proteins that extensively remodel the host cell. Three such proteins are pUL21, pUL7 and pUL51. pUL21 is a viral phosphatase adapter. pUL7 and pUL51 form a complex …
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An in vitro chemogenomic screening system to identify the molecular targets of antimalarial drug candidates
The efficacy of existing antimalarials is threatened by parasite resistance, driving the need for a well-stocked pipeline of potential replacement therapies. A global effort to carry out phenotypic screening in live parasite assays has identified many promising hit compounds, but elucidating the …
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Structural and functional characterisation of pUL21, an α-herpesvirus phosphatase adaptor protein
… impact viruses can have upon our lives. Virus infection is a complex process where viral proteins cooperate to exploit host cell metabolism while evading immune responses. Herpes simplex virus 1 (HSV-1) is a very prevalent human herpesvirus that causes life-long infection and is known to …
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