Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 43 for “"in vitro differentiation"”.
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The Role of EphA Receptor Signaling in Neuroblast Survival During In Vitro Differentiation
… embryonic stem cells can be differentiated into multiple cell types and can serve as an excellent model for studying developmental processes in vitro. In particular, stem cells can be differentiated into forebrain-like neurons, allowing investigation of nervous system development at single …
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Eomes Directs the Formation of Spatially and Functionally Diverse Extra-Embryonic Mesoderm Derived Tissues
During mouse embryonic development, the first mesodermal cells to originate at the posterior primitive streak will give rise to the extra-embryonic mesoderm (ExEM). These cells will contribute to key tissues supporting the development of the embryo, including the amnion, chorion, yolk-sac, and …
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Consequences of Plk4 overexpression in mouse pancreatic organoids
Overexpression of the polo-like kinase 4 (Plk4), the master regulator of centrosome duplication, leads to centrosome amplification, a common feature of cancer, including pancreatic cancer. Previous studies have shown that overexpression of Plk4 in mice carrying a doxycycline-inducible Plk4 …
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Investigating the cytoplasmic role of E2F4 in multiciliogenesis
… factors plays essential transcriptional roles in many cellular processes including proliferation and terminal differentiation. Transgenic mouse models have established that E2F4 is necessary for multiciliogenesis in the airway epithelia. Here we show that E2F4 plays two distinct roles in …
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Investigating the role of the Polycomb Repressive Complex 2 in human in vitro pancreatic differentiation
The potential to use human pluripotent stem cells in regenerative medicine is an idea that continues to excite and captivate much of the science community, decades after the initial discovery of human embryonic stem cells. This interest has grown with the discovery of induced human pluripotent stem …
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Silver Nanoparticles As Drug Delivery Systems
… applications of silver nanoparticles (SNPs) in the medical device and pharmaceutical industries. Bone cements have been used in the orthopedic surgeries for many years to anchor prosthetics while filling up the spaces between the prostheses and bone. Antibiotics are an active component of …
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The Biological Effects of Novel Synthetic Retinoids
Naturally occurring Retinoids are known to play a role in many developmental processes in the early embryo as well as being a potent tool for in vitro differentiation of stem cells. The exogenous application of these naturally occurring retinoids as well as some of their synthetic analogs has been …
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Gene expression and imprinting in mice cloned by nuclear transfer
… mammals derived by nuclear transfer (NT) die during gestation, and those surviving to birth frequently show increased birth weights, enlarged dysfunctional placentas, and neonatal mortality associated with respiratory distress and metabolic abnormalities. These abnormalities may reflect …
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Bioluminescence Imaging Strategies for Tissue Engineering Applications
In vitro differentiation of stem cells in biocompatible scaffolds in a bioreactor is a promising method for creating functional engineered tissue replacements suitable for implantation. Basic studies have shown that mechanical, chemical, and pharmaceutical stimuli enhance biological functionality …
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Analysis of human cytomegalovirus in the healthy human carrier
Much circumstantial evidence has pointed to peripheral blood leukocytes as one site of persistence of Human Cytomegalovirus (HCMV) in healthy carriers. However, the exact population of peripheral blood cells that carry HCMV and to what extent they express HCMV gene products in not known. I have …
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Modelling the gastric epithelium for testing of new chemical entities
… would prove useful for biopharmaceutical screening of new chemical entities and dosage forms. A successful model should exhibit tight junction formation, maintenance of differentiation and polarity. Conditions for primary culture of guinea-pig gastric mucous epithelial cell monolayers on Tissue …
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Machine Aided Biological Discovery and Design
Advances in biotechnology and the life sciences are primarily driven by biologists conducting rigorous experimentation. However, biology is often too complex – with intractable combinatorial search spaces and functional landscapes – to comprehensively explore, understand, and engineer via iterative …
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Extracellular matrix based substrates for propagation of human pluripotent stem cells
In human pluripotent stem cell (hPSC) research and applications, the need for a culture system devoid of non-human components is crucial. Such a system should exhibit characteristics observed in conventional culture systems that have used mouse embryonic fibroblast feeders for hPSC self renewal …
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The Role of AF9 and AF9-Mediated Protein Interactions in Hematopoiesis and Leukemogenesis
<p>The AF9 protein is one of the most common chromosomal translocation partners of the MLL gene in MLL leukemia. Wild-type AF9 is a member of the pTEFb transcription elongation complex, and interacts with gene regulatory proteins such as AF4/AF5q31, DOT1L, Pc3/CBX8 and BCoR. These interactions are …
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Impacts of dietary obesity on muscle stem cell behaviors
Occurrence of obesity has steadily increased in the human population and, along with it, associated health complications such as systemic insulin resistance, which can lead to the development of type 2 diabetes mellitus. Obesity is a complex metabolic disorder that often leads to chronic …
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Runx1 in Primitive Hematopoiesis and Characterization of Hematopoietic Stem Cells in a Mouse Chronic Inflammatory Arthritis Model
… different aspects of hematopoiesis are addressed in this dissertation. I. <underline>The regulation of primitive hematopoiesis by Runx1 and TGF-beta signaling.<underline> Primitive hematopoiesis, occurring exclusively in the yolk sac, is characterized by its transient nature. As the primitive …
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Effects of HOXB4 downstream targets on the haemopoietic differentiation of pluripotent stem cells
Attempts for the in vitro differentiation of reconstituting human HSCs from ESCs have been unsuccessful as key factors of HSC specification remain unclear. Enforced HOXB4 expression can enhance haemopoietic differentiation of mouse and human ESCs and generate reconstituting HSCs from mouse ESCs. We …
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Neuronale in-vitro Differenzierung von murinen homozygoten Pbx1 Knock-out embryonalen Stammzellen : ein System zum Studium der Funktion und zur Identifikation potentieller Zielgene des Pbx1-Homeoboxproteins während der zellulären Differenzierung
… früh während der embryonalen Entwicklung mit einer Aplasie und Hypoplasie multipler Organsysteme. Dies verhindert eine detaillierte Analyse des Effektes eines PBX1-K.O.s auf die neuronale Entwicklung, die deshalb von besonderem Interesse ist, weil PBX1 embryonal auch in Regionen aktiver …
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Inflammation-associated gene regulation in primary astrocytes, glial tumors and cellular differentiation
This dissertation elucidates several independent molecular mechanisms that function in astrocytes and glial tumor cells, and suggest that developmental and inflammatory signals may contribute to the development of brain tumors. First, we analyzed the mechanism of TIMP-1 activation in astrocytes and …
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Analysis of an epigenetic regulator in mouse embryonic stem cell self-renewal and differentiation
… Mll and Trx2, for the Drososphila protein Trithorax (TRX), which is the founding member of the trithorax group (TrxG) of epigenetic regulators. TrxG proteins are characterized by an evolutionary conserved SET domain. A major function of all SET domain- containing proteins is to modulate …
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