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Showing 1 to 20 of 129 for “"in vitro culture"”.
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Design and Characterization of a Continuous in Vitro Culture System
Made available in DSpace on 2014-12-09T14:29:03Z (GMT). No. of bitstreams: 1 6901474.pdf: 5629060 bytes, checksum: e64c666b7ee69ea4fc1bbb1d7b9cb425 (MD5) Previous issue date: 1968
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Bud-forcing and in vitro culture for conservation of oak (Quercus L.)
… for this genus can be complex and challenging, especially since Quercus species are considered “recalcitrant” due to the inability of their seeds, acorns, to survive seed banking, an important conservation method. One method to support conservation efforts is in vitro culture (tissue …
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EFFECTS OF THE LIVER MICROENVIRONMENT ON HUMAN LIVER SINUSOIDAL ENDOTHELIAL CELLS DURING IN VITRO CULTURE
Liver Sinusoidal Endothelial Cells (LSECs) are specialized endothelial cells that play an important role in the metabolic and xenobiotic clearance functions of the liver. LSECs are distinguished by their hallmark characteristics of lacking a distinct basement membrane and containing open pores …
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The chick embryo amnion as an in vitro culture system for IVF and NT embryos
Calving rates are significantly reduced following in vitro production of embryos. Thus, if a technique could be developed that would increase calving rates by as little as one viable offspring, significant research advances could be made. Therefore, in a series of experiments, the efficiency and …
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In vitro culture of a chondrocyte-seeded peptide hydrogel and the effects of dynamic compression
Emerging medical technologies for effective and lasting repair of articular cartilage include delivery of cells or cell-seeded scaffolds to a defect site to initiate de novo tissue regeneration. Biocompatible scaffolds assist in providing a template for cell distribution and extracellular matrix …
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Development and characterization of an in vitro culture system as a physiological model for chronic Hepatitis B infection
… for viral replication. Although a vaccine has been available for more than 20 years chronic HBV infection afflicts 350-400 million worldwide. It is estimated that 0.5-1.2 million people die each year from HBV-attributable cases of chronic hepatitis, cirrhosis, and hepatocellular …
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Greenhouse, laser radiation, and in vitro culture studies on the growth and propagation of Algerian ivy (Hedera canariensis L.)
… of this research demonstrated that the combination of benzylaminopurine (BA) + gibberellic acid (GA$\sb{4 + 7}$) (Promalin) promoted branching of Hedera canariensis (Algerian Ivy) better than applications of BA or GA$\sb{4 + 7}$ alone. Moreover, as the concentration of Atrinal is increased, …
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The effects of magnetic field (MFs) on in vitro culture of human umbilical cord derived mesenchymal stem cells (hUC-MSCs)
… effects of magnetic field (MF) exposures on individual have been extensively studied. However, a number of studies have surprisingly observed that response to optimal MF has significantly increased the proliferation rate of stem cells. Accordingly, this current study aims to investigate the …
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In vitro culture studies of environmental factors and the effects of copper on a fouling alga, Cladophora glomerata (L) Kützing, which occurs in South African freshwater systems
… glomerata (L.) Kütz., is problematic as a fouling organism in eutrophic freshwater systems in South Africa. A general consensus among scientific researchers is that C. glomerata thrives under high light conditions, temperatures between 15° C and 25° C, high inorganic nutrient concentrations, …
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Comparison of two different media and assisted hatching techniques on the embryo hatching rate using the mouse as a model
The use of in vitro culture media and assisted hatching techniques remain a challenging obstacle to hatching of blastocyst-stage embryos. Mechanical, chemical, enzymatic thinning and laser assisted techniques have been used previously, but there is still a lack of information on its application and …
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Energy substrates, metabolic regulators, and lipid accumulation during culture of in vitro-produced bovine embryos
The main objective of this experiment was to optimize in vitro culture conditions for bovine embryonic development, using alternative energy sources and metabolic regulators. Replacing glucose with fructose in culture medium consistently increased blastocyst production per oocyte and decreased …
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OVARIAN TISSUE BANKING IN ANIMAL MODELS
… offers limitless opportunity of fertility insurance for all ages and at any state of reproductive cycle in addition to multiple restoration options that can rekindle both reproductive and endocrine functions of the gonad. Vitrification has been argued as a superior cryopreservation …
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Genetic analysis of tissue culture responses in barley (Hordeum vulgare L.).
… this thesis were to screen barley genotypes for in vitro culture response; and to use the double haploid (DH) lines derived from the cross between responsive and unresponsive parental lines to confirm the location of previously reported QTL that control barley tissue response and search for …
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Use of gene expression to characterize heterogeneous liver cell populations
Non-parenchymal cells (NPC's) are integral to recreate the native hepatic microenvironment and necessary to maintain in vivo liver function. A variety of in vitro culture systems have been developed to address different aspects of liver physiology and architecture in order to recreate the …
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Characterizing an AraC-Differentiated, Heterogeneous NT2-Derived Population
… by one or more neurological diseases. The initial stages of new pharmacotherapy identification rely heavily on in vitro or in vivo studies (e.g., animals or animal tissues such as primary cultures of isolated neurons)—the latter of which entails significant financial, labor, and time …
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Identification, characterization and amino terminal sequencing of high molecular weight soluble antigens of the human malaria parasite, Plasmodium falciparum
… of four species of protozoa that cause malaria in humans. Since the blood stage of the parasite life cycle is responsible for the clinical manifestations of the disease, studies of the immune response and vaccine development to this stage are critical to decreasing the morbidity and mortality of …
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Characterization of porcine adipose tissue-derived stem cells
Nuclear transfer (NT) results in low success rates and research has reported problems with the welfare of cloned animals produced. Evidence suggests the use of a less differentiated donor cell, such as a stem cell, may lead to an increase in the efficiency of the NT procedure. Stem cell …
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Somatic cell interspecies nuclear transfer
… of oocytes to produce embryos and live offspring. A series of experiments were conducted to evaluate the ability of the bovine cytoplast to reprogram nuclei from horses and llamas. In a preliminary study, equine oocytes from small (<20mm diameter) follicles were either pretreated with …
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