Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 99 for “"in vitro assay"”.
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Design of an in vitro assay to optimize assembly of nanoparticle-tagged nuclear import complexes
Maintaining protein function at the biological-inorganic interface is a critical challenge for bionanotechnology. Specifically, nanoparticle-protein conjugates must be designed to interact with binding partners with biologically-relevant thermodynamics. Towards developing a nanoparticle-tagging …
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Evaluation of Particle Size Differences, Calcium Sources, and Phytases on Ph and Calcium and Phosphorus Solubility in an in Vitro Simulation of Broiler Digestion
… Feed enzymes, different Ca sources, and various ingredient particle sizes all contribute to broiler nutritional status and need to be investigated to determine their full effects on the bird. The objective of the first study was to evaluate various Ca sources and microbial phytases using an in …
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THE IDENTIFICATION OF NOVEL INORGANIC CARBON SENSITIVE ENZYMES
… the formation of the second messenger adenosine-3’, 5’-monophosphate from adenosine triphosphate, and is involved in a number of diverse signalling pathways in eukaryotes and prokaryotes. Adenylyl cyclases are diverse in their structure and biochemistry, and have been grouped into six …
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In Vitro Evidence For A Spleen-Derived Macrophage Suppressor Factor
… mice are more resistant than normal mice to infection by Listeria monocytogenes. This appears to be due to a splenic suppressor factor which inhibits phagocytosis by macrophages. An in vitro assay was developed which used monolayer cultures on microscope cover slips and direct microscopic …
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Characterization of an IL-12-driven Anticancer Response, and the CD4+ CTL Population Incited, in a Murine Model of Leukaemia
… the treatment of cancer, immunotherapy has some inherent advantages over other treatment modalities: disseminated disease can be eradicated due to the systemic nature of immunity, the immune system is effective against a wide range of targets, long-term memory can offer added protection against …
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Wheat Take-All: Quantification of Resistance and Pseudomonad Suppression and the Potential for Their Interaction
A new 10-day in vitro assay was developed to study the dynamic colonization of Ggt-infected roots by both antagonistic and non-antagonistic bacteria. Pseudomonas fluorescens (strain 2-79), as antagonist of Ggt and Bacillus megaterium (strain B153-2-2), a non-antagonist, were used to determine if …
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Tyrosine Sulfation in A431 Cells
In this thesis research I have identified and characterized a unique tyrosine sulfotransferase and its substrate in A431 cells. I also established an in vitro assay method for tyrosine sulfotransferase activity, which opened a door towards enzyme purification Sulfation of proteins on tyrosine …
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Ena/VASP proteins regulate vertebrate nervous system development : a thesis presented
… These processes depend upon regulation of the actin cytoskeleton. Ena/VASP proteins are able to remodel the actin cytoskeleton in response to extracellular signals and have been shown to regulate the motility and morphology of a variety of cells. I have investigated the in vivo requirement for the …
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Molecular genetic studies of bacteroides fragilis
Some genetic systems operative in Bacteroides fragilis have been successfully investigated. Firstly the bacteriocin produced by the B.fragilis BF-1 strain was purified and partially characterized. This bacteriocin was found to be cell bound and constitutively produced by the bacteria. The purified …
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Characterization of the Murine Macrophage Suppressor Factor (Msf)
Lymphokines are soluble substances produced primarily by lymphocytes and affect specific aspects of the immune process. Macrophage suppressor factor (MSF) is a lymphokine produced in the mouse spleen and is present in plasma. The in vitro target cell is the macrophage. The macrophage does not …
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Investigation into peptidoglycan biosynthetic enzymes from the Lyme disease spirochete Borrelia burgdorferi
… disease, has unique chemical characteristics within its peptidoglycan (PG), such as the incorporation of L-Ornithine. This unusual structural feature may be implicated in immune evasion and may contribute to chronic symptoms such as Lyme arthritis and Post-Treatment Lyme Disease Syndrome (PTLDS). …
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A study of nutrient distributions and productivity potential in lake sediments
The purpose of this investigation was to study the sediment in the upper reaches of Claytor Lake. The lake is a manmade impoundment on the New River near Radford, Virginia. Eleven core samples, varying in length from 66 cm. to 141 cm, were taken from the lake. The concentration of various water …
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Aggregation of IgG mAb Biotherapeutics: Sources, Methods of Characterization, and Biological Implications
One of the predominant concerns with protein therapeutics is their tendency to aggregate at various stages of protein production, purification, filling, transportation, and administration. This occurrence has biological significance; while there is no definite, general cause and effect relationship …
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Trifluoromethyl ketones: Potential insecticides towards Anopheles gambiae
Malaria continues to cause significant mortality in sub-Saharan Africa and elsewhere, and existing vector control measures are being threatened by growing resistance to pyrethroid insecticides. With the goal of developing new human-safe, resistance-breaking insecticides we have explored several …
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Studies of thiamine biosynthesis
In Escherichia coli, and other prokaryotes, thiamine (vitamin B1) is assembled by coupling 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate (Hmp-PP) and 4-methyl-5-(?-hydroxyethyl)thiazole phosphate (Thz-P). The thiazole moiety is biosynthesised from 1-deoxyxylulose-5-phosphate, tyrosine, …
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Functional Roles of Nova in RNA Metabolism
<p>Nova-1 is a neuron-specific RNA binding protein, distributed both in the nucleus and the cytoplasm of several populations of neurons. The work presented here confirms that Nova-1 binds a sequence of RNA found in an intron of the glycine receptor a2 subunit pre-mRNA. Evidence that Nova-1 acts as …
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Association Among CCR5 Genotypes, CCR5 Expression, And In Vitro HIV Infection
The single nucleotide polymorphisms (SNPs) in CCR5 (-2459 G>A [promoter], ¿32 deletion polymorphism [open reading frame, ORF]) influences the expression of CCR5 chemokine receptor on human T lymphocytes. CCR5 is a major coreceptor for HIV to bind to the T cell prior to fusion. Individuals have …
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Chitin Synthesis -Related Proteins in the Chitin -Less Yeast Schizosaccharomyces Pombe
I identified and characterized four chitin synthesis-related proteins in S. pombe. Two of these, SpChs1p and SpChs2p are homologs of S. cerevisiae chitin synthases and two, SpChs4p and SpChs5p, are homologs of S. cerevisiae proteins that regulate, respectively, the activity and localization of …
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Protein and Pathway Engineering for Biosynthesis or Aromatic Compounds
… from Penicillium patulum, were successfully engineered via rational pathway design to synthesize TAL in vivo with a maximal yield of 1.7 g/L. The direct biosynthesis of phloroglucinol from D-glucose was achieved by the discovery of a novel type III polyketide synthase, PhlD, from Pseudomonas …
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