Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 102 for “"in situ hybridisation"”.
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Optimisation of interphase fluorescence in situ hybridisation for detection of common aneuploidies
… of a simple, reliable and practical method of interphase FISH which allows prenatal diagnosis of major chromosome aneuploidies using a minimum volume of amniotic fluid sample was the overall objective of this study. When all the probes required were available, the study continued by developing …
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The added value of BAP1 immunohistochemistry and fluorescence in situ hybridisation for CDKN2A/p16 and NF2 in the diagnosis and prognostication of pleural mesothelioma
… palliative. The malignancy can be challenging to diagnose, commonly due to scant samples, often requiring patients to undergo multiple procedures before a definitive diagnosis can be reached. In more recent years ancillary tests based on the common molecular alterations in the disease have …
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Arc expression in the parabrachial nucleus following taste stimulation
… however molecular mechanisms of taste processing in the early structures of the central taste pathway remain unclear. More recently the Arc catFISH (cellular compartment analysis of temporal activity by fluorescent in situ hybridisation) method has been used in our lab to study neural …
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The Genetic Aetiology of Ectopic Maxillary Canine Teeth
Introduction. The ectopic canine (EC) is a common clinical complication of dental development appearing in 1-2% of the Western population The aetiology is controversial with opinion divided as to a genetic or environmental mechanism. This study addresses the hypothesis that genetic factors play an …
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Central vasopressin signalling and aggressive behaviour
Although many signalling molecules appear relevant to the production of complex behaviours, those that are important to the physiological regulation of behaviour, and so those that characterise individual styles of behaviour, are unknown. Vasopressin is the strongest candidate regulator of social …
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Molecular mapping of the rabbit atrioventricular node
… complex. Ionic currents have been characterised in the AVN at both the whole tissue level and single cell level. However, little is known about the molecular basis of these ionic currents. There were two aims of this research: 1) to generate an accurate three-dimensional reconstruction of the …
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Molecular detection of the apicomplexan parasite 'X' (APX) in bivalves in New Zealand
… Ostrea chilensis is an iconic seafood species in New Zealand that forms the basis of one of the oldest commercial fisheries in the country and is under development for aquaculture. However, the productivity of commercially important flat oyster populations has been severely hampered by an …
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USE OF MOLECULAR APPROACHES TO STUDY THE OCCURRENCE OF EXTREMOPHILES AND EXTREMODURES IN NON-EXTREME ENVIRONMENTS
… environments. A range of molecular approaches, in addition to classical microbiology techniques, were used to isolate microorganisms, mainly archaea and bacteria from unusual environments. Halo-bacteria and archaea from non-saline environments and alkaliphilic bacteria and archaea capable of …
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Mikrobielle Ökologie N-Acyl-L-Homoserinlacton-produzierender Bakterien in der Rhizosphäre von Tomatenpflanzen
N-Acylhomoserinlactones (AHL) are signalling molecules in gram-negative bacteria, which regulate, in a cell density dependent way, important interactive functions. This phenomenon is known as quorum-sensing. This work characterised the microbial ecology of the autoinducer (AHL) producing bacteria …
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CELL DIVISION AND EMBRYOGENIC COMPETENCE IN ALFALFA {Medicago falcata L.)
This investigation exploited a procedure for the production of somatic embryos directly from alfalfa leaf explants. Chopped leaf fragments were incubated in a liquid medium containing 2,4-D. The organisation of the swollen leaf tissue was disrupted and after 15 days the globular clusters of small …
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Investigating the cell surface code for compartment boundary formation in Drosophila embryos
During animal development, the separation of cells of different identity is crucial for the formation and function of tissues and organs. Tissues are compartmentalised to ensure organisation remains during dynamic processes, such as cell division and cell rearrangement. At Drosophila compartment …
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Halogenated aliphatic and aromatic aerobic biodegradation via direct metabolism and cometabolism: batch and continuous tests
In this thesis the application of biotechnological processes based on microbial metabolic degradation of halogenated compound has been investigated. Several studies showed that most of these pollutants can be biodegraded by single bacterial strains or mixed microbial population via aerobic direct …
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Spatial organisation of proto-oncogenes in human haematopoietic progenitor cells
… is a highly organised organelle, with distinct specialised sub- compartments responsible for specific nuclear functions. Within the context of this functional framework, the genome is organised, allowing contact between specific genomic regions and sub-compartments. Previous work has shown …
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Patterns of Gene Expression in Schistosoma mansoni larvae associated with Infection of the Mammalian Host
Larval schistosomes infect the human host by penetration of unbroken skin, before gaining access to a blood vessel and beginning their intravascular life. The work in this thesis focuses on the mammalian infection process, as it would be the ideal point to interrupt the life cycle. A whole organism …
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Acquired abnormalities of chromosome 21 in acute lymphoblastic leukaemia
The intrachromosomal amplification of chromosome 21 (iAMP21) was identified<br/>as a novel and prognositically important acquired chromosomal abnormality in<br/>childhood acute lymphoblastic leukaemia (ALL). It is defined by multiple copies<br/>of the RUNX1 gene, as seen by fluorescence in situ …
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Regulation of Notch Ligands Dll1 and Jag1 by Pax6 during cortical development
The regulation of gene expression resulting in the formation of the mammalian cerebral cortex is tightly regulated by a group of transcription factors. The deletion of any one of these transcription factors results in numerous defects whose nature and severity depends on the role of the …
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Stromal interactions in pleural mesothelioma
Pleural mesothelioma (PM) is a devastating malignancy primarily related to past asbestos exposure. There are no curative options, and 1-year survival stands currently at just 40%. The genomic landscape in mesothelioma is dominated by loss of function of tumour suppressor genes rather than …
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Endodermal Patterning in Xenopus laevis
The endoderm is the inner germ layer of the vertebrate embryo from which the respiratory and digestive systems are derived. These include organs such as the liver, pancreas, stomach, lungs and intestine. Recent research has helped our understanding of early vertebrate endoderm specification and …
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Modelling metabolic landscapes in human naive and primed embryonic stem cells
… mammalian embryos undergo remarkable changes in their metabolism, with a global transition from oxidative phosphorylation to glycolysis as they transit out of pluripotency and begin to make the first cell fate decisions in preparation for gastrulation. In humans this transition can be modelled …
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Axial patterning of the chondrichthyan pharyngeal endoskeleton and the origin of the jaw
… (sharks, skates and holocephalans), but remain largely untested from a developmental perspective. Here, I test 1) whether jaws and gill arches ancestrally shared common developmental patterning mechanisms, and 2) whether the mandibular arch might carry anatomical vestiges of an ancestral …
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