Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 188 for “"immunostaining"”.
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Automatic GUI Based Counting Software for Immunostaining Analysis Using Matlab GUIDE
Typically, researchers need to the manually count the number of cells in many histological sections. However, this manual counting method is labor intensive and time consuming. Manual counting is also subject to bias, where two individuals (who are blinded to each other’s results) normally count …
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Prognostic factors in soft tissue sarcoma. Tissue microarray for immunostaining, the importance of whole-tumor sections and time-dependence.
In adult soft tissue sarcoma (STS) of the extremities and trunk wall, improved prognostic factors are needed to identify patients at high-risk for metastasis. Various factors are included in the many prognostic systems currently in use and the prognostic value of immunohistochemical (IHC) …
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Green tea extract and epigallocatechin gallate decrease muscle pathology and NF-κB immunostaining in regenerating muscle fibers of mdx mice
Duchenne muscular dystrophy is a debilitating genetic disorder characterized by severe muscle wasting and early death in affected boys. The primary cause of this disease is mutations in the dystrophin gene resulting in the loss of the dystrophin protein from the plasma membrane of muscle fibers. In …
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A New Immunoassay for Quantification of a Novel Cancer Antigen in Serum and Immunostaining of Carcinoma Tissues and Cultured Cells Revealing the Antigenic Cellular Location.
<p>The purpose of this study is 1) to examine the presence of the antigen in serum by employing a newly developed ELISA immunoassay that quantifies the total antigen and bound antigen (antigen-antibody complex) using polyclonal chicken antibodies directed against an IgM-binding epitope of the new …
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Effect of Photoperiod on Developmental Morphology and Enolase Isoenzyme Immunohistochemistry in Rat and Djungarian Hamster Superficial Pineal Glands
… pinealocytes demonstrates a mosaic pattern of immunostaining for the NSE antigen. Furthermore, an increasing proximal to distal immunostaining gradient has been reported in the gland. In this study, it was hypothesized that structural and immunohistochemical differences represent pinealocyte …
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Antibody technology identifies distinct mechanisms controlling the lengths of filopodia-like structures
… Western blotting, immunoprecipitation and immunostaining approaches with different proteomic techniques and transient expression. The antibody panel was strikingly split into two groups: (1) Antibodies that lengthen FLS and do not recognise specific proteins in their eluates or (2) …
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The expression of Heat Shock Protein 70 in the placental tissue with preeclampsia
… decidual cell. And according to the intensity of immunostaining, we compared these three groups. Western blot analysis was performed and the quantification and comparison of Hsp70 were done in placental tissue among the three group of patients. RESULTS: All groups demonstrated positive …
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Analysis of the Role of Akirin Interactions with Nuclear Body Proteins During Myogenesis
… Body (HLB). Through <em>Drosophila</em> embryo immunostaining for myosin heavy chain and green fluorescent protein, interactions between Akirin and Mute, a component of the HLB, as well as interactions between Akirin and Coilin, a component of both the CB and the HLB, were observed. Using …
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Characterization and Functional Analysis of the Arabidopsis Intron Splicing Factor ASF/SF2
… of SF2a and RRM domain, but not SF2b protein. Immunostaining of TN-5 cells expressing recombinant proteins and western blot analyzing cytoplasmic and nuclear fractions of expression cells showed targeting of SF2a, SF2b and their SR domains to the nuclei. SR domain, possibly required …
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Placental co-culture model reveals Listeria monocytogenes utilization of monocytes for vertical transmission
… barrier. Barrier integrity was confirmed using immunostaining of tight junctions and a FITC-dextran permeability assay. The model was then used to observe L. monocytogenes use of the “Trojan horse” method for vertical transmission. Human monocyte THP-1 cells were exposed to L. monocytogenes (MOI …
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Paracrine control of glucagon secretion in the pancreatic α-cell: Studies involving optogenetic cell activation
… compared with commercially available antibodies. Immunostaining of intact islets showed an islet-wide network involving α- and δ-cells. Furthermore, we used immunostaining to compare the islet architecture as pertaining to δ-cell number, and morphology between islets from healthy human donors and …
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Defensins In Ocular Immunity
… <p>infection, and their potential synergy. Immunostaining and real-time RT-PCR data</p> <p>demonstrated that their expression was either constitutive (mBD1 and mBD2) or</p> <p>inducible (mBD3 and mBD4) in normal BALB/c and B6 corneas, and disparately</p> <p>regulated in BALB/c vs B6 corneas …
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Regulation of P-glycoprotein at the Blood-Brain Barrier
… but not by an ET-A receptor antagonist. Immunostaining localized the ET-B receptor to the luminal and abluminal membranes of brain capillaries. NBD-CSA transport was also reduced by the NO donor, sodium nitroprusside (SNP), and by the protein kinase C (PKC) activator, PMA. Inhibition of …
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Sumo and Ubiquitin Ligases Regulate Hepatocyte Nuclear Factor - 1 Beta Transcriptional Activity
… their stability and transcriptional activity. Immunostaining of the adult mouse kidney showed that HNF-1β co-localized with Ubc9, PIAS1, and PIASgamma in the nuclei of renal tubular epithelial cells. Immunostaining also showed that Arkadia localized to the nuclei of renal tubules. …
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The immunodetection of cocaine for forensic applications.
… the position of the cocaine during subsequent immunostaining. The performance of the method was determined using newly-minted UK banknotes as true negative sample matrices, resulting in staining which could be readily distinguished from that on newly-minted notes spiked with cocaine. The method …
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TOLL-LIKE RECEPTOR EXPRESSION AND FUNCTION
… was examined by RT-PCR, flow cytometry or immunostaining. Cells were exposed to AMPs or TLR agonists to determine TLR mRNA expression or the antimicrobial activity of the culture media against PA. (2) TLR mRNA or protein expression was examined by real-time RT- PCR or western blotting in …
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Correlation of Cyclooxygenase-2 and Microvessel Density with Prognosis in Transitional Cell Carcinoma of the Upper Urinary Tract
… antibodies against COX-2 and CD34. COX-2 immunostaining was semi-quantitatively estimated based on proportion and intensity and then immunoreactive scores for each case were generated by multiplying the values for the two parameters. COX-2 immunostaining was considered to be positive if …
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The Regulatory Mechanism of Dietary Pattern in TMJ Condyle Growth
… Radiographic analysis, cell proliferation, immunostaining, and cell lineage tracing were performed. Results: Soft diet mice exhibited significantly shorter mandibular length, reduced chondrogenic activity, and fewer chondrocyte-derived bone cells in the condylar head and mid-condylar process …
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Estudo da modulação da migração de células hospedeiras induzida por Leishmania e seu impacto na disseminação do parasito no hospedeiro
… assessed the formation of adhesion complexes by immunostaining p-FAK and p-paxillin. Additionally, we investigated actin dynamics by analyzing the expression of Rac1, Rho-A, Cdc42, and phalloidin staining in these cells, as well as the formation of podosomes by immunostaining vinculin. Finally, …
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