Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 588 for “"immunoprecipitation"”.
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Reproducibility and reliability of chromatin immunoprecipitation in clinical research
… expression via histone modifications. Chromatin immunoprecipitation (ChIP) is an experimental technique used to investigate the interactions between histones and DNA. ChIP determines the specific location in the genome that histone modifications are associated with, indicating the target of the …
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The developmental function of Pax7 : chromatin-immunoprecipitation discovery of Pax7 target genes
Pax7 plays critical roles in development of brain, spinal cord, neural crest and skeletal muscle. As a sequence-specific DNA-binding transcription factor, the direct functional role played by Pax7 during development is the selection of target genes. To date, an accurate description of the function …
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Identification of the Pokeweed Antiviral Protein Interactome by Co-Immunoprecipitation-Mass Spectrometry
… the processes in which it is involved. Co-immunoprecipitation coupled with mass spectrometry was used to identify PAP protein interactors in pokeweed. Results identified protein interactions with diverse cellular functions in both the extracellular matrix, where PAP is primarily localized, …
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Microfluidic Technology for Low-Input Epigenomic Analysis
… and tumorigenesis. Chromatin immunoprecipitation (ChIP) is the technique of choice for examining in vivo DNA-protein interactions and has been a great tool for studying epigenetic mechanisms. However, conventional ChIP assays require millions of cells for tests and are not …
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Microfluidic Engineering for Ultrasensitive Molecular Analysis of cells
… and fast molecular analysis of cells. Chromatin immunoprecipitation (ChIP) assay followed by next generation sequencing serves as the primary technique to characterize the genomic locations associated with histone modifications. However, conventional ChIP-seq assay requires large numbers of …
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Signals Regulating Organelle Transport in Melanophores
… of motors on the organelles were examined. Immunoprecipitation of myosin V showed that the motor subunit is more heavily phosphorylated in cells aggregating pigment compared to cells dispersing pigment. Furthermore, levels of myosin V on melanosomes are higher in cells dispersing pigment. …
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Proteomic analysis of TRIF interactome and functional characterisation of novel TRIF interacting proteins
… modulate the functionality of TRIF. To this end, immunoprecipitation and LC-MS analyses of the overexpressed and endogenous TRIF immunocomplexes were performed. A disintegrin and metalloproteinase domain-containing protein 15 (ADAM15), Segment polarity protein dishevelled homolog DVL3 (DVL3) and …
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Association of Protein Phosphatase 2A with S6 Kinase Is Regulated In an MTOR-Dependent Manner
… This was supported by studies showing the co-immunoprecipitation of the catalytic subunit of protein phosphatase 2A (PP2A) with S6K1. PP2A is a ubiquitously expressed phosphatase that has been implicated in many intracellular signaling pathways. In this dissertation, an association between …
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Identification and Characterization of the DNA Damage Response Function of Human Rif1 and a Search For Human Rap1 Interacting Factors
… of human Rifl with telomeres. Preliminary co-immunoprecipitation with known telomeric proteins and chromatin immunoprecipitation failed to show an interaction of human Rifl with telomeric protein complexes or with telomeric DNA. Unexpectedly, Rifl responded to ionizing radiation (IR), UV …
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Motifs, binding, and expression : computational studies of transcriptional regulation
… like transcriptional profiling and chromatin immunoprecipitation have emerged and taken on increasing importance in the study of regulatory processes. Mining these experiments for useful biological information requires methods that can handle large quantities of noisy data and integrate …
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Analysis of the interaction between the co-chaperone p23 and the aryl hydrocarbon receptor
… and analyzed for proper refolding via co-immunoprecipitation with the known binding partner SRC-1. This AhR NΔ515 transactivation domain construct was used, along with amino terminal AhR deletion constructs AhR CΔ274 and AhR CΔ553, to attempt to elucidate the nature of the interaction …
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Expression and regulation of genes associated with CNS regeneration and plasticity
… extraction for MeDIP-seq 14 5. Methylated DNA immunoprecipitation and sequencing 15 6. Bioinformatics analysis 15 7. Bisulfite sequencing 17 8. Chromatin immunoprecipitation assay 18 9. ChIP-PCR primers 19 10. qRT-PCR primers 20 11. Methylation assay 21 12. Tissue processing and …
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Role of the ubiquitin-proteasome pathway in the inner ear : identification of an E3 ubiquitin ligase for Atoh1
… tag to a specific protein substrate. Using an immunoprecipitation/mass spectrometry screening approach, we identified Huwe1, a HECT domain E3 ubiquitin ligase, as an Atoh1 binding partner. We validated the binding between Atoh1 and Huwe1 through reciprocal co-immunoprecipitation and mass …
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Modulation of neuronal ryanodine receptor-mediated calcium signaling by calsenilin
… immunoblotting, immunohistochemistry and co-immunoprecipitation. Mechanisms of intracellular Ca2+- signaling controlled by the interaction of calsenilin and RyR, including changes in the release of Ca2+ from intracellular stores, were measured with single channel electrophysiology and …
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Identification of echinus and characterization of its role in Drosophila eye development
… A protein interaction screen using an immunoprecipitation and mass spectrometry approach identified interactions between Echinus and the vesicle coat protein Clathrin, the scaffolding protein RACK1 and the casein kinase I epsilon (Dco). Co-immunoprecipitation additionally identified an …
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Lysine acetyltransferase Gcn5-B regulates the expression of crucial genes in Toxoplasma and its function is regulated through lysine acetylation
… parasites, which were identified using chromatin immunoprecipitation coupled with microarray hybridization (ChIP-chip). We also demonstrate that GCN5b interacts with AP2-domain proteins, which are plant-like transcription factors in Apicomplexa. The interactions between GCN5b, AP2IX-7, and AP2X-8 …
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Biochemical and immunological investigations into cystic fibrosis
… The antisera produced were tested by immunoprecipitation, immunofluorescent and radiolabelling techniques. Immunoprecipitation and immunofluorescence showed that, after absorption, the antisera produced from fibroblast medium had specificity for CF samples. Sadiolabelling experiments …
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Maintenance of Genomic Integrity by RSF1 Chromatin Remodeler
… and purification of recombinant proteins 5 E. Immunoprecipitation and in vitro binding assay 5 F. In vitro kinase assay 6 G. Cell fractionation 6 H. Chromosome spreading 7 I. Immunocytochemistry 7 J. Live cell imaging 7 III. RESULTS 9 A. RSF1 localizes at mitotic kinetochores and directly …
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Defining the functional role of MAGE-C1 in Multiple Myeloma
… similarity was investigated through co-immunoprecipitation, to determine MAGE-C1 interaction with RBX1 or SKIP, and immunofluorescence microscopy, to assess co-localisation of these proteins. Lastly, cellular pathways influenced by MAGE-C2 were evaluated using heat shock to induce a …
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